FastQCFastQC Report
Fri 10 Feb 2017
ERR1632060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28947
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT530.18309323936850105No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT420.145092755725982RNA PCR Primer, Index 17 (95% over 21bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT390.13472898745984038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380.13127439803779323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA601.2637138E-624.6666661
GTATCAA652.535915E-622.769232
GGCTGGA500.00687427818.51
ATCAACG801.529746E-518.54
CAACGCA902.0086045E-618.56
TCAACGC852.577602E-517.4117645
TATCAAC852.577602E-517.4117643
CTCTTAT800.006104921513.87537
AACGCAG1153.393047E-412.8695657
GTCTCTT1653.4546301E-612.33333337
AGAGTAC1256.851822E-411.8412
CGCAGAG1256.851822E-411.849
CAGAGTA1309.522754E-411.38461511
GCAGAGT1309.522754E-411.38461510
ACGCAGA1350.001305976610.9629628
TGTCTCT2251.06664746E-49.04444436
CTGTCTC2751.03434075E-48.07272736
Produced by FastQC (version 0.11.5)