FastQCFastQC Report
Fri 10 Feb 2017
ERR1632059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314576
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5920.188189817405015No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT3160.10045267280402827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT453.9960996E-628.77777736
GGTATCA1950.025.6153831
TTTAGAC400.001928086623.1250023
TTAAGAG502.69606E-422.23
TAGACTG450.003819725720.5555555
CAAATAC450.003819725720.5555553
ATGCCGT555.1316887E-420.18181834
CCGTCTT609.216498E-418.537
ATACCGC609.216498E-418.527
GTATCAA2750.018.1636372
CGTATGC650.00157648717.07692331
TACCGCA650.00157648717.07692328
TCTTATA3000.016.03333337
GCTGCAT2450.015.85714212
TATGCCG700.00258688515.85714233
ATCAGAA2350.015.74468117
CAGGACA2500.015.544
AAGAGGC2400.015.41666722
ATCAAGC2300.015.28260930
CCATCAA2300.015.28260928
Produced by FastQC (version 0.11.5)