FastQCFastQC Report
Fri 10 Feb 2017
ERR1632058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56305
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14202.52197850990143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12882.2875410709528463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8081.4350412929579968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.8738122724447208No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT3860.6855519048041914TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT3080.5470206908800285No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.29482283988988545No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1600.2841665926649498No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1530.2717343042358583No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG1380.24509368617351923No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC1280.22733327413195983RNA PCR Primer, Index 11 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.22733327413195983No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.21312494449871236No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1190.21134890329455644No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA900.15984370837403428No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA900.15984370837403428No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG870.15451558476156646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.1420832963324749No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA780.13853121392416304No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.13675517272000712No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG730.12965100790338335No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA720.1278749666992274No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC720.1278749666992274No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA700.12432288429091555No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.12432288429091555No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA670.11899476067844775No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA670.11899476067844775No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC640.11366663706597992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG640.11366663706597992No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC620.11011455465766806No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC600.10656247224935618No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10656247224935618No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT590.10478643104520026No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA590.10478643104520026No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG570.10123434863688838No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10123434863688838No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAA570.10123434863688838No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA570.10123434863688838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGT200.001827109537.026
GGACGTA200.001827109537.027
AAAGTGG251.2187757E-436.99999622
GAAAGTG303.556555E-430.83333421
CTCTGCA250.005452607329.5999989
ACACGAA250.005452607329.59999817
CAGCACT250.005452607329.5999984
AAACACG250.005452607329.59999815
TGCAAAC250.005452607329.59999812
ACGTATA250.005452607329.59999829
AGTGGAC250.005452607329.59999824
TCTGCAA250.005452607329.59999810
GACGTAT250.005452607329.59999828
AAGTGGA250.005452607329.59999823
GCACTCT250.005452607329.5999986
GTGGACG250.005452607329.59999825
ACGAAAG250.005452607329.59999819
TACACAT604.1713065E-827.7500025
GCCGTCT508.892292E-625.89999836
CACACTG601.3000754E-624.66666833
Produced by FastQC (version 0.11.5)