##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56305
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1143592931356	33.0	31.0	34.0	30.0	34.0
2	32.22001598437084	33.0	31.0	34.0	30.0	34.0
3	32.18458396234793	34.0	31.0	34.0	30.0	34.0
4	35.869212325725954	37.0	35.0	37.0	35.0	37.0
5	35.65042180978599	37.0	35.0	37.0	33.0	37.0
6	35.71927892727111	37.0	35.0	37.0	35.0	37.0
7	35.66294290027528	37.0	35.0	37.0	33.0	37.0
8	35.70766361779593	37.0	35.0	37.0	33.0	37.0
9	37.361868395346775	39.0	37.0	39.0	34.0	39.0
10	37.2573483704822	39.0	37.0	39.0	34.0	39.0
11	37.36295178048131	39.0	37.0	39.0	35.0	39.0
12	37.29365065269514	39.0	37.0	39.0	34.0	39.0
13	37.37183198650209	39.0	37.0	39.0	35.0	39.0
14	38.38739010745049	40.0	38.0	41.0	34.0	41.0
15	38.37394547553503	40.0	38.0	41.0	34.0	41.0
16	38.25226889263831	40.0	38.0	41.0	34.0	41.0
17	38.37387443388687	40.0	38.0	41.0	34.0	41.0
18	38.508924607050886	40.0	38.0	41.0	35.0	41.0
19	38.53563626676139	40.0	38.0	41.0	34.0	41.0
20	38.58033922386999	40.0	38.0	41.0	35.0	41.0
21	38.5714945386733	40.0	38.0	41.0	35.0	41.0
22	38.45296154870793	40.0	38.0	41.0	34.0	41.0
23	38.385045733061006	40.0	38.0	41.0	34.0	41.0
24	38.31423497025131	40.0	38.0	41.0	34.0	41.0
25	38.19126187727555	40.0	37.0	41.0	34.0	41.0
26	37.998454844152384	40.0	37.0	41.0	34.0	41.0
27	37.82687150341888	40.0	37.0	41.0	33.0	41.0
28	37.693437527750646	39.0	36.0	41.0	33.0	41.0
29	37.51423497025131	39.0	36.0	40.0	33.0	41.0
30	37.22832785720629	39.0	35.0	40.0	33.0	41.0
31	36.93735902672942	39.0	35.0	40.0	32.0	41.0
32	36.6870082585916	38.0	35.0	40.0	32.0	41.0
33	36.43143592931356	38.0	35.0	40.0	31.0	41.0
34	36.26209040049729	38.0	35.0	40.0	31.0	41.0
35	36.11723647988633	38.0	35.0	40.0	31.0	41.0
36	35.92769736257881	38.0	35.0	40.0	30.0	41.0
37	35.68468164461415	38.0	35.0	40.0	30.0	41.0
38	35.45779238078323	38.0	35.0	40.0	28.0	41.0
39	35.16520735281058	38.0	35.0	40.0	26.0	41.0
40	34.800763697717784	38.0	34.0	40.0	24.0	41.0
41	34.45203800728177	38.0	34.0	40.0	22.0	41.0
42	34.12327501998046	37.0	33.0	40.0	20.0	41.0
43	33.1129917414084	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	14.0
21	26.0
22	39.0
23	84.0
24	110.0
25	188.0
26	271.0
27	452.0
28	611.0
29	812.0
30	1098.0
31	1357.0
32	1731.0
33	2203.0
34	3040.0
35	4106.0
36	6506.0
37	11027.0
38	14309.0
39	8316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02983749222982	17.68759435218897	16.61308942367463	21.66947873190658
2	19.77621880827635	23.18621792025575	32.49622591244117	24.54133735902673
3	21.42438504573306	24.289139508036588	33.25992363022822	21.026551816002133
4	14.83349613711038	19.08711482106385	30.171387976201046	35.908001065624724
5	13.80694432110825	37.62010478643105	31.432377231151765	17.14057366130894
6	29.341976733860225	32.81946541159755	17.708906846638843	20.129651007903384
7	24.53245715300595	34.24385045733061	19.69096883047687	21.53272355918657
8	31.780481307166326	30.206908800284165	18.767427404315782	19.245182488233727
9	27.283544978243494	12.487345706420388	19.13862001598437	41.090489299351745
10	20.731728976112247	27.510878252375452	26.260545244649673	25.49684752686262
11	32.197850990142975	24.187905159399698	17.66450581653494	25.949738033922387
12	20.548796732084185	32.055767693810495	26.026107805701095	21.36932776840423
13	34.26516295178048	18.861557588136044	25.562561051416395	21.310718408667082
14	22.934020069265607	23.704821951869285	27.287097060651806	26.074060918213306
15	29.446763164905427	25.9248734570642	23.232394991563805	21.395968386466567
16	22.688926383092088	24.955154959595063	28.340289494716274	24.01562916259657
17	19.07823461504307	31.528283456176183	26.74007636977178	22.65340555900897
18	19.962703134712726	22.84521800905781	30.761033655980818	26.431045200248647
19	21.38176005683332	27.514430334783768	32.52641861291182	18.577390995471095
20	23.445519936062517	22.323061895035963	33.48725690436018	20.74416126454134
21	26.454133735902673	22.25557232927804	31.128674185241096	20.16161974957819
22	25.198472604564426	24.695852943788296	29.310007992185415	20.79566645946186
23	21.77071308054347	24.985347660065713	30.794778438859783	22.449160820531038
24	21.16685907113045	26.676138886422162	31.890595861823996	20.266406180623388
25	20.99635911553148	26.580232661397744	30.137643193322084	22.285765029748692
26	22.97131693455288	23.884202113489035	31.196163750999023	21.94831720095906
27	21.435041292957997	26.1184619483172	30.077257792380784	22.369238966344017
28	20.266406180623388	25.02797264896546	33.03436639730042	21.67125477311074
29	20.93597371459018	25.090134091110915	33.05035076813782	20.923541426161087
30	20.637598792291982	26.152206731196166	33.98099635911554	19.229198117396322
31	20.58964567977977	26.83243051238789	32.43228842909156	20.145635378740785
32	19.564869904981798	27.85898232839002	33.1356007459373	19.44054702069088
33	19.346416836870613	26.45590977710683	33.55119438770979	20.646478998312762
34	19.500932421632182	27.590800106562476	31.624189681200605	21.28407779060474
35	18.957463813160466	28.033034366397303	32.735991475002216	20.273510345440013
36	19.222093952579698	27.84122191634846	30.439570198028594	22.49711393304325
37	18.289672320397834	28.317200959062248	30.97060651807122	22.422520202468696
38	18.961015895568778	26.235680667791495	32.379007192966874	22.424296243672853
39	19.591510523044136	25.84672764408134	32.95089246070509	21.610869372169432
40	18.758547198295002	25.56078501021224	33.265251753840694	22.415416037652072
41	17.215167391883494	26.271201491874614	33.45351212148122	23.06011899476068
42	17.28620904004973	26.052748423763433	33.620459994671876	23.04058254151496
43	16.55092798152917	26.814670100346326	33.84424118639552	22.790160731728974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	14.0
2	25.0
3	70.0
4	115.0
5	115.0
6	190.5
7	266.0
8	284.0
9	302.0
10	416.5
11	531.0
12	531.0
13	994.0
14	1457.0
15	2194.0
16	2931.0
17	2407.5
18	1884.0
19	1884.0
20	2106.5
21	2329.0
22	1543.5
23	758.0
24	705.0
25	652.0
26	652.0
27	637.0
28	622.0
29	639.5
30	657.0
31	727.0
32	797.0
33	797.0
34	835.5
35	874.0
36	932.5
37	991.0
38	1242.5
39	1494.0
40	1494.0
41	1740.5
42	1987.0
43	2489.0
44	2991.0
45	3548.0
46	4105.0
47	4105.0
48	4965.5
49	5826.0
50	5568.5
51	5311.0
52	4539.0
53	3767.0
54	3767.0
55	3596.5
56	3426.0
57	3272.0
58	3118.0
59	2571.0
60	2024.0
61	2024.0
62	1852.0
63	1680.0
64	1534.0
65	1388.0
66	1305.0
67	1222.0
68	1222.0
69	1109.5
70	997.0
71	851.0
72	705.0
73	601.0
74	497.0
75	497.0
76	402.0
77	307.0
78	230.5
79	154.0
80	117.0
81	80.0
82	80.0
83	52.0
84	24.0
85	15.5
86	7.0
87	4.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	56305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.77248912174762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67142980362841	54.156824438326964
2	6.736436560190909	8.322529082674718
3	1.9205888272332594	3.559186573128496
4	0.9574192806417297	2.3656868839357075
5	0.5318996003565165	1.6428381138442412
6	0.39676835053621234	1.4705621170411154
7	0.2875132974900089	1.2432288429091556
8	0.2041344412179063	1.0087914039605719
9	0.17250797849400534	0.9590622502442057
>10	0.9919208763405308	11.361335582985525
>50	0.08912912222190275	3.58405114998668
>100	0.03162646272390098	4.081342687150342
>500	0.002875132974900089	1.4350412929579968
>1k	0.005750265949800178	4.809519580854276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1420	2.52197850990143	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1288	2.2875410709528463	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	808	1.4350412929579968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	492	0.8738122724447208	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	386	0.6855519048041914	TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	308	0.5470206908800285	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.29482283988988545	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	160	0.2841665926649498	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	153	0.2717343042358583	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	138	0.24509368617351923	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	128	0.22733327413195983	RNA PCR Primer, Index 11 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128	0.22733327413195983	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.21312494449871236	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	119	0.21134890329455644	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.15984370837403428	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	90	0.15984370837403428	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	87	0.15451558476156646	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80	0.1420832963324749	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	78	0.13853121392416304	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77	0.13675517272000712	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	73	0.12965100790338335	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	72	0.1278749666992274	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	72	0.1278749666992274	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	70	0.12432288429091555	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.12432288429091555	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	67	0.11899476067844775	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	67	0.11899476067844775	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	64	0.11366663706597992	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	64	0.11366663706597992	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	62	0.11011455465766806	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	60	0.10656247224935618	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.10656247224935618	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	59	0.10478643104520026	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA	59	0.10478643104520026	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	57	0.10123434863688838	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.10123434863688838	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAA	57	0.10123434863688838	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA	57	0.10123434863688838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0017760412041559364	0.0
15	0.0	0.0	0.0	0.0017760412041559364	0.0
16	0.0	0.0	0.0	0.007104164816623746	0.0
17	0.0	0.0	0.0	0.01598437083740343	0.0
18	0.0	0.0	0.0017760412041559364	0.021312494449871235	0.0
19	0.0	0.0	0.0017760412041559364	0.023088535654027173	0.0
20	0.0	0.0	0.0017760412041559364	0.026640618062339045	0.0
21	0.0	0.0	0.0017760412041559364	0.03374478287896279	0.0
22	0.0	0.0	0.0017760412041559364	0.046177071308054346	0.0
23	0.0	0.0	0.0017760412041559364	0.06571352455376965	0.0
24	0.0	0.0	0.0017760412041559364	0.09057810141195276	0.0
25	0.0	0.0	0.0017760412041559364	0.09945830743273244	0.0
26	0.0	0.0	0.0017760412041559364	0.111890595861824	0.0
27	0.0	0.0	0.0017760412041559364	0.1420832963324749	0.0
28	0.0	0.0	0.0017760412041559364	0.2593020158067667	0.0
29	0.0	0.0	0.0017760412041559364	0.41204155936417725	0.0
30	0.0	0.0	0.0017760412041559364	0.7068643992540627	0.0
31	0.0	0.0	0.0017760412041559364	1.2503330077257793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGT	20	0.0018271095	37.0	26
GGACGTA	20	0.0018271095	37.0	27
AAAGTGG	25	1.2187757E-4	36.999996	22
GAAAGTG	30	3.556555E-4	30.833334	21
CTCTGCA	25	0.0054526073	29.599998	9
ACACGAA	25	0.0054526073	29.599998	17
CAGCACT	25	0.0054526073	29.599998	4
AAACACG	25	0.0054526073	29.599998	15
TGCAAAC	25	0.0054526073	29.599998	12
ACGTATA	25	0.0054526073	29.599998	29
AGTGGAC	25	0.0054526073	29.599998	24
TCTGCAA	25	0.0054526073	29.599998	10
GACGTAT	25	0.0054526073	29.599998	28
AAGTGGA	25	0.0054526073	29.599998	23
GCACTCT	25	0.0054526073	29.599998	6
GTGGACG	25	0.0054526073	29.599998	25
ACGAAAG	25	0.0054526073	29.599998	19
TACACAT	60	4.1713065E-8	27.750002	5
GCCGTCT	50	8.892292E-6	25.899998	36
CACACTG	60	1.3000754E-6	24.666668	33
>>END_MODULE