FastQCFastQC Report
Fri 10 Feb 2017
ERR1632057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445896
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.0054927829.65
GGTATCA2600.028.4615381
TAAGATT450.00382219120.5555554
TCCAGTA656.890026E-519.9230772
GCCGTCT951.670378E-719.47368436
GTATTAA609.2247303E-418.51
GCGGTTA500.007028294318.513
GAATAGT609.2247303E-418.51
ATAAGTT500.007028294318.54
GTATCAA4000.018.52
CAGTCGG609.2247303E-418.59
GGACCGC500.007028294318.56
CGCAACC500.007028294318.512
TAGAGAG650.001577884217.0769235
CTAATAC650.001577884217.0769233
CTCGTAT1107.781273E-716.81818229
TATGCCG1107.781273E-716.81818233
CTTATAC4850.016.78350437
CGTATGC1151.2379514E-616.08695631
TCTTATA7550.015.92715137