##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632057.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445896
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83673322927319	34.0	31.0	34.0	31.0	34.0
2	33.01350763406714	34.0	33.0	34.0	31.0	34.0
3	33.09984615246604	34.0	33.0	34.0	31.0	34.0
4	36.47796347130272	37.0	37.0	37.0	35.0	37.0
5	36.34668398012092	37.0	37.0	37.0	35.0	37.0
6	36.36795799917469	37.0	37.0	37.0	35.0	37.0
7	36.351010101010104	37.0	37.0	37.0	35.0	37.0
8	36.3316019879075	37.0	37.0	37.0	35.0	37.0
9	38.14274853329027	39.0	39.0	39.0	37.0	39.0
10	38.11013554730251	39.0	38.0	39.0	37.0	39.0
11	38.18342618009581	39.0	39.0	39.0	37.0	39.0
12	38.14013357374814	39.0	38.0	39.0	37.0	39.0
13	38.163719791162066	39.0	39.0	39.0	37.0	39.0
14	39.5755669483467	41.0	39.0	41.0	37.0	41.0
15	39.59201921524301	41.0	39.0	41.0	37.0	41.0
16	39.59159983493909	41.0	39.0	41.0	37.0	41.0
17	39.57519690690206	41.0	39.0	41.0	37.0	41.0
18	39.56776916590416	41.0	39.0	41.0	37.0	41.0
19	39.594977304124725	41.0	39.0	41.0	37.0	41.0
20	39.56943547374276	41.0	39.0	41.0	37.0	41.0
21	39.55087284927427	41.0	39.0	41.0	37.0	41.0
22	39.51557313813086	41.0	39.0	41.0	37.0	41.0
23	39.47399617848108	40.0	39.0	41.0	37.0	41.0
24	39.44092792938264	41.0	39.0	41.0	37.0	41.0
25	39.384446148877764	40.0	39.0	41.0	37.0	41.0
26	39.317031325690294	40.0	39.0	41.0	36.0	41.0
27	39.24493379622154	40.0	39.0	41.0	36.0	41.0
28	39.19905089976138	40.0	39.0	41.0	36.0	41.0
29	39.139761289628076	40.0	39.0	41.0	36.0	41.0
30	39.0823241293934	40.0	39.0	41.0	36.0	41.0
31	39.02777777777778	40.0	39.0	41.0	35.0	41.0
32	38.94255835441448	40.0	38.0	41.0	35.0	41.0
33	38.879054757163104	40.0	38.0	41.0	35.0	41.0
34	38.80948920824587	40.0	38.0	41.0	35.0	41.0
35	38.79056551303443	40.0	38.0	41.0	35.0	41.0
36	38.7693161634103	40.0	38.0	41.0	35.0	41.0
37	38.718660853651976	40.0	38.0	41.0	35.0	41.0
38	38.68807076089492	40.0	38.0	41.0	35.0	41.0
39	38.65616421766511	40.0	38.0	41.0	35.0	41.0
40	38.59400398299155	40.0	38.0	41.0	35.0	41.0
41	38.55806062400201	40.0	38.0	41.0	35.0	41.0
42	38.517033119830636	40.0	38.0	41.0	35.0	41.0
43	37.666850566051274	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	3.0
19	10.0
20	15.0
21	35.0
22	62.0
23	103.0
24	171.0
25	268.0
26	423.0
27	720.0
28	992.0
29	1455.0
30	2100.0
31	2980.0
32	4196.0
33	6236.0
34	9708.0
35	14815.0
36	25038.0
37	45866.0
38	112958.0
39	217737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.213888440353806	17.165213412993165	11.967364587258016	27.653533559395015
2	19.657722877083444	19.284765954392952	32.75405027181226	28.303460896711343
3	20.18475160127025	20.30922008719522	28.969086961982164	30.53694134955236
4	15.531200100471859	14.77519421569155	32.68901268457219	37.0045929992644
5	16.93646051994187	33.87830346089672	32.72601682903637	16.45921919012505
6	37.42083355760087	32.878070222652816	14.609236234458258	15.09185998528805
7	31.98750381254822	28.477043974379672	19.46036743994115	20.075084773130953
8	29.62058417209394	31.495460824945727	18.71019251125823	20.173762491702103
9	28.10431131923139	13.270583275023773	17.746514882394102	40.87859052335074
10	18.081794857993792	25.233686778979852	31.404183935267415	25.280334427758937
11	39.49956940631896	19.532805856074063	19.375370041444643	21.592254696162332
12	24.092837791772073	23.370920573407254	27.166424457721085	25.36981717709959
13	33.41474245115453	17.511706765703213	21.961174803093098	27.11237598004916
14	24.608204603764104	18.25111685236019	22.978452374544737	34.162226169330964
15	27.51471195076879	25.03386439887328	20.209196763370834	27.242226886987098
16	27.456626657337136	23.611559646195527	21.729954967077525	27.201858729389812
17	26.231677341801674	24.115040278450582	23.00401887435635	26.649263505391392
18	26.67886682096274	22.829762994061394	23.82237113587025	26.66899904910562
19	27.402578179665216	22.98764734377523	23.38975904695265	26.220015429606903
20	27.64972101117749	22.616260293880185	23.077802895742504	26.656215799199813
21	28.194466871198664	22.767865152412224	22.454339128406627	26.58332884798249
22	28.175628397653263	23.039004610940665	22.30564974792328	26.479717243482785
23	27.63379801568079	22.63644437267883	22.471831996698782	27.257925614941602
24	27.068643809318765	22.83873369574968	22.725030051850656	27.367592443080895
25	27.096228717010245	22.2184096740047	22.97037874302528	27.714982865959776
26	26.913674937653624	22.55862353553295	23.0087284927427	27.518973034070726
27	26.88698710013097	22.772350503256362	23.138803667222852	27.201858729389812
28	26.942381183056142	22.60751385973411	23.1372337944274	27.312871162782354
29	26.57839496205393	23.088343470226242	23.462870265712183	26.870391302007647
30	26.332822003337103	23.360604266465725	23.030482444336798	27.27609128586038
31	26.993962717763782	22.934495936272135	22.690044315266338	27.381497030697744
32	25.956052532429087	23.259459604930296	23.327412670219065	27.457075192421556
33	25.942596479896658	22.866094335898953	23.907368534366757	27.28394064983763
34	26.4833055241581	22.26954267362793	24.034079695713796	27.21307210650017
35	25.990365466386784	22.559969140786194	24.674139261173007	26.775526131654022
36	25.849076914796278	22.455236198575452	24.21685774261263	27.47882914401564
37	25.51805802249852	21.847246891651864	24.89055743940291	27.744137646446703
38	24.817894755727792	21.77122019484364	25.864327107666362	27.546557941762206
39	24.783806089312304	21.134748910059745	26.62504261083302	27.45640238979493
40	24.316208263810395	20.788479824891905	27.63334948059637	27.26196243070133
41	23.6671779966629	20.62409171645406	28.295387265191884	27.413343021691155
42	23.20585966234279	20.452302779123382	29.106338697812944	27.235498860720885
43	22.44200441358523	19.833324362631647	29.551509769094135	28.173161454688984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	14.5
4	24.0
5	24.0
6	38.0
7	52.0
8	47.0
9	42.0
10	55.0
11	68.0
12	68.0
13	120.5
14	173.0
15	303.0
16	433.0
17	442.5
18	452.0
19	452.0
20	620.0
21	788.0
22	880.5
23	973.0
24	1284.5
25	1596.0
26	1596.0
27	2118.5
28	2641.0
29	3423.5
30	4206.0
31	5217.5
32	6229.0
33	6229.0
34	7732.0
35	9235.0
36	10903.5
37	12572.0
38	14454.0
39	16336.0
40	16336.0
41	18441.5
42	20547.0
43	22496.0
44	24445.0
45	26438.5
46	28432.0
47	28432.0
48	29979.5
49	31527.0
50	32952.0
51	34377.0
52	36072.0
53	37767.0
54	37767.0
55	37923.0
56	38079.0
57	37464.0
58	36849.0
59	35508.0
60	34167.0
61	34167.0
62	31738.5
63	29310.0
64	26391.5
65	23473.0
66	20581.0
67	17689.0
68	17689.0
69	14919.5
70	12150.0
71	10257.0
72	8364.0
73	6791.0
74	5218.0
75	5218.0
76	4205.0
77	3192.0
78	2585.0
79	1978.0
80	1639.0
81	1300.0
82	1300.0
83	978.5
84	657.0
85	478.0
86	299.0
87	222.0
88	145.0
89	145.0
90	109.5
91	74.0
92	47.0
93	20.0
94	13.5
95	7.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	445896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.16954463926072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70587908318252	73.62554901285769
2	5.888370609720566	9.55912722116449
3	1.4767104427906326	3.595917426160702
4	0.6226055256935292	2.021464280417252
5	0.33125936535242356	1.344408592157336
6	0.22251606930816245	1.0836916812397035
7	0.14977340565311062	0.8509927395153987
8	0.11039287926920495	0.7168431793358602
9	0.07928212562117587	0.5791764631233159
>10	0.3960547770010131	5.418747367577776
>50	0.012451729895441105	0.656506600861348
>100	0.004703986512289933	0.5475754355891782
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2426754220715144E-4	0.0
5	0.0	0.0	0.0	2.2426754220715144E-4	0.0
6	0.0	0.0	0.0	2.2426754220715144E-4	0.0
7	0.0	0.0	0.0	2.2426754220715144E-4	0.0
8	0.0	0.0	0.0	2.2426754220715144E-4	0.0
9	0.0	0.0	0.0	6.728026266214543E-4	0.0
10	0.0	0.0	0.0	0.0013456052532429086	0.0
11	0.0	0.0	0.0	0.0015698727954500601	0.0
12	0.0	0.0	0.0	0.0017941403376572115	0.0
13	0.0	0.0	0.0	0.0017941403376572115	0.0
14	0.0	0.0	0.0	0.002018407879864363	0.0
15	0.0	0.0	0.0	0.0022426754220715144	0.0
16	0.0	0.0	0.0	0.0024669429642786658	0.0
17	0.0	0.0	0.0	0.002691210506485817	0.0
18	0.0	0.0	0.0	0.003588280675314423	0.0
19	0.0	0.0	0.0	0.004036815759728726	0.0
20	0.0	0.0	0.0	0.004261083301935877	0.0
21	0.0	0.0	0.0	0.004485350844143029	0.0
22	0.0	0.0	0.0	0.005158153470764483	0.0
23	0.0	0.0	0.0	0.006055223639593089	0.0
24	0.0	0.0	0.0	0.007849363977250301	0.0
25	0.0	0.0	0.0	0.008970701688286058	0.0
26	0.0	0.0	0.0	0.011661912194771876	0.0
27	0.0	0.0	0.0	0.032518793620036957	0.0
28	0.0	0.0	0.0	0.11863752982758312	0.0
29	0.0	0.0	0.0	0.2480399016811095	0.0
30	0.0	0.0	0.0	0.4063727864793584	0.0
31	0.0	0.0	0.0	0.85737481385794	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	25	0.00549278	29.6	5
GGTATCA	260	0.0	28.461538	1
TAAGATT	45	0.003822191	20.555555	4
TCCAGTA	65	6.890026E-5	19.923077	2
GCCGTCT	95	1.670378E-7	19.473684	36
GTATTAA	60	9.2247303E-4	18.5	1
GCGGTTA	50	0.0070282943	18.5	13
GAATAGT	60	9.2247303E-4	18.5	1
ATAAGTT	50	0.0070282943	18.5	4
GTATCAA	400	0.0	18.5	2
CAGTCGG	60	9.2247303E-4	18.5	9
GGACCGC	50	0.0070282943	18.5	6
CGCAACC	50	0.0070282943	18.5	12
TAGAGAG	65	0.0015778842	17.076923	5
CTAATAC	65	0.0015778842	17.076923	3
CTCGTAT	110	7.781273E-7	16.818182	29
TATGCCG	110	7.781273E-7	16.818182	33
CTTATAC	485	0.0	16.783504	37
CGTATGC	115	1.2379514E-6	16.086956	31
TCTTATA	755	0.0	15.927151	37
>>END_MODULE