FastQCFastQC Report
Fri 10 Feb 2017
ERR1632056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380779
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.12512711175627808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.028.3612351
CTTATAC27950.018.93023337
GTATCAA18500.017.12
TAGACGT904.4491753E-516.4444454
TCTTATA48550.016.2327537
ATCTCGC6450.014.91472811
ATGCGTT1651.6474587E-814.57575736
CGAATTA1152.2117E-514.47826115
TCGCCAT5150.014.36893213
TCTATAC2500.014.0599993
TTAGTAC1458.9207424E-714.0344833
GTTCTAG6100.013.950821
AACGCTA950.001245759813.63157933
TTGCGCG3150.013.507936518
CTAGCAC4250.013.4941183
TTCCTCG7150.013.45454619
ATTAGAC1254.8486443E-513.323
GTATTAT2102.044544E-913.2142851
GTATAAC850.00940859913.0588241
TACGTTA1000.001833483312.94999919
Produced by FastQC (version 0.11.5)