##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2380779
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47641087223972	34.0	31.0	34.0	31.0	34.0
2	32.65325424997448	34.0	31.0	34.0	31.0	34.0
3	32.7410154407444	34.0	31.0	34.0	31.0	34.0
4	36.23392805464094	37.0	37.0	37.0	35.0	37.0
5	36.05815869511618	37.0	35.0	37.0	35.0	37.0
6	36.078536058995816	37.0	35.0	37.0	35.0	37.0
7	36.05396384964753	37.0	35.0	37.0	35.0	37.0
8	36.03442528684939	37.0	35.0	37.0	35.0	37.0
9	37.77912691602202	39.0	37.0	39.0	35.0	39.0
10	37.71231517079074	39.0	37.0	39.0	35.0	39.0
11	37.794333283349694	39.0	37.0	39.0	35.0	39.0
12	37.7531144217922	39.0	37.0	39.0	35.0	39.0
13	37.774236079871336	39.0	37.0	39.0	35.0	39.0
14	39.104597276773696	40.0	38.0	41.0	36.0	41.0
15	39.10309524739591	40.0	38.0	41.0	36.0	41.0
16	39.094988237043424	40.0	38.0	41.0	36.0	41.0
17	39.08322948077079	40.0	38.0	41.0	36.0	41.0
18	39.07577393785815	40.0	38.0	41.0	36.0	41.0
19	39.10834394960641	40.0	39.0	41.0	36.0	41.0
20	39.089961310982666	40.0	39.0	41.0	36.0	41.0
21	39.055768721078266	40.0	39.0	41.0	36.0	41.0
22	39.01906308817408	40.0	39.0	41.0	36.0	41.0
23	38.96961876763866	40.0	38.0	41.0	36.0	41.0
24	38.92504554181635	40.0	38.0	41.0	35.0	41.0
25	38.869082766607065	40.0	38.0	41.0	35.0	41.0
26	38.78294163380978	40.0	38.0	41.0	35.0	41.0
27	38.707223139989054	40.0	38.0	41.0	35.0	41.0
28	38.63928193251033	40.0	38.0	41.0	35.0	41.0
29	38.5736114103829	40.0	38.0	41.0	35.0	41.0
30	38.50268966586147	40.0	38.0	41.0	34.0	41.0
31	38.43310655881962	40.0	38.0	41.0	34.0	41.0
32	38.34491987706545	40.0	38.0	41.0	34.0	41.0
33	38.27693330628336	40.0	38.0	41.0	34.0	41.0
34	38.20348171753867	40.0	38.0	41.0	34.0	41.0
35	38.17670014730473	40.0	38.0	41.0	34.0	41.0
36	38.15224344636776	40.0	38.0	41.0	34.0	41.0
37	38.105239503540645	40.0	38.0	41.0	34.0	41.0
38	38.06164956932164	40.0	38.0	41.0	33.0	41.0
39	38.02510606822389	40.0	38.0	41.0	33.0	41.0
40	37.96223252977282	40.0	37.0	41.0	33.0	41.0
41	37.926142241678036	40.0	37.0	41.0	33.0	41.0
42	37.88539633456108	40.0	37.0	41.0	33.0	41.0
43	37.01156512217219	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	1.0
14	2.0
15	6.0
16	13.0
17	28.0
18	84.0
19	90.0
20	232.0
21	412.0
22	761.0
23	1268.0
24	2044.0
25	3182.0
26	4832.0
27	7015.0
28	10534.0
29	14850.0
30	20294.0
31	27354.0
32	37154.0
33	50466.0
34	71452.0
35	104241.0
36	166234.0
37	303709.0
38	708351.0
39	846165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83773462383531	18.585387387909588	12.854490063966459	26.722387924288647
2	18.393139388410265	20.531473101871278	34.8199475885834	26.25543992113506
3	19.50823658978847	21.632415272480145	30.41038248405249	28.448965653678897
4	15.014665367932093	15.64408120199313	34.229804614372014	35.11144881570276
5	15.530126903841138	35.29298603524308	33.747945525393156	15.428941535522616
6	34.78575709883194	34.71481393275058	15.71712452100762	14.78230444740986
7	30.00139870185347	30.24115216070034	20.683986207875655	19.073462929570532
8	27.335212550177907	33.443381347029685	19.985391336197104	19.236014766595304
9	27.096341155562946	14.523607609106095	19.206738634707378	39.173312600623575
10	17.110828010495723	26.427358440241616	32.600379959668665	23.861433589593993
11	36.455042656206224	21.175379991170956	21.37115624759795	20.998421105024867
12	22.46252172083171	24.766137470130577	28.50457770334836	24.266763105689357
13	30.87917862178724	19.00798016111533	24.16549373125351	25.94734748584392
14	23.408136580505793	20.073135725743548	24.2677291760386	32.25099851771206
15	25.874724197416054	26.78640058569065	21.743345350408415	25.595529866484878
16	25.72179106082505	25.64110318513394	23.213662418897343	25.423443335143663
17	24.6327777588764	25.682056167330103	24.70250283625654	24.982663237536958
18	24.404953168689744	24.64827688752295	25.76778441006074	25.178985533726568
19	25.33431284466135	24.828428006127407	25.624763995314137	24.212495153897105
20	25.79176815655716	24.383615614889077	25.100565823203247	24.724050405350518
21	25.956420146515068	24.956453328931413	24.10891561123481	24.97821091331871
22	26.21184074624314	24.655333401378286	24.09723876092657	25.035587091452
23	25.35027400695319	24.661633860177698	24.456994958372867	25.53109717449625
24	25.603090417044168	24.576871687796306	24.533650540432355	25.286387354727168
25	25.48653192925509	23.927042367225184	24.84384312865663	25.742582574863103
26	25.073179828955144	24.588842559515182	24.84863147734418	25.489346134185492
27	25.343175490039183	24.40717933079887	24.570109195351606	25.679535983810343
28	24.831662241644437	24.859048235892537	24.987367580107183	25.32192194235584
29	24.717371919023144	25.075237978829616	25.115098881500554	25.09229122064669
30	24.637188080035987	25.54046385657804	24.77252193504731	25.049826128338665
31	25.11694701608171	24.950992931305258	24.579055846846767	25.353004205766265
32	24.117820259671312	24.754040589235707	25.20721158914792	25.920927561945064
33	24.35580959005435	24.353163397358596	25.61296113582991	25.678065876757145
34	25.05049817727727	23.795824811962806	25.71381047967913	25.439866531080796
35	24.78113256207317	23.84551443036082	26.112587518623105	25.260765488942905
36	24.19254370103231	23.87441253472078	26.175256082147904	25.75778768209901
37	24.558768369512666	22.65325761021918	26.493471254576757	26.294502765691398
38	23.60983526820423	22.84915987582216	27.637634572549572	25.90337028342404
39	23.526291184524055	22.262587161597107	28.237312241077394	25.97380941280144
40	22.99692663619765	22.310806672941926	29.090646380869455	25.601620309990974
41	22.313452865637675	22.238099378396736	29.83510859260771	25.613339163357875
42	22.35167564902076	22.004898396701247	29.734553270169133	25.908872684108857
43	21.274843234084308	21.655390945568655	30.329526596126733	26.740239224220307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	82.0
2	72.0
3	234.5
4	397.0
5	397.0
6	527.0
7	657.0
8	680.5
9	704.0
10	1048.0
11	1392.0
12	1392.0
13	2240.0
14	3088.0
15	5151.0
16	7214.0
17	7612.0
18	8010.0
19	8010.0
20	9674.5
21	11339.0
22	13151.0
23	14963.0
24	18630.5
25	22298.0
26	22298.0
27	26452.5
28	30607.0
29	39369.5
30	48132.0
31	55456.0
32	62780.0
33	62780.0
34	71707.5
35	80635.0
36	90404.5
37	100174.0
38	110189.0
39	120204.0
40	120204.0
41	130637.5
42	141071.0
43	142426.5
44	143782.0
45	150023.0
46	156264.0
47	156264.0
48	160906.5
49	165549.0
50	172892.5
51	180236.0
52	188712.0
53	197188.0
54	197188.0
55	185873.0
56	174558.0
57	167004.5
58	159451.0
59	147361.0
60	135271.0
61	135271.0
62	125950.5
63	116630.0
64	103622.5
65	90615.0
66	78802.0
67	66989.0
68	66989.0
69	57679.5
70	48370.0
71	41987.0
72	35604.0
73	29102.0
74	22600.0
75	22600.0
76	18030.0
77	13460.0
78	11050.5
79	8641.0
80	6965.5
81	5290.0
82	5290.0
83	4112.5
84	2935.0
85	2394.5
86	1854.0
87	1442.5
88	1031.0
89	1031.0
90	757.0
91	483.0
92	296.0
93	109.0
94	66.5
95	24.0
96	24.0
97	19.0
98	14.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2380779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.55147895971932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.10345039552325	54.48449644734528
2	7.794559402962012	9.751224369893212
3	2.041440401486015	3.830853489632202
4	0.8794078191723403	2.200330387918852
5	0.4883913139993805	1.527479950087096
6	0.3111793099065461	1.1678835633791578
7	0.21088844590706485	0.9233968930902571
8	0.1584618249384963	0.7929617206847261
9	0.11374311621636561	0.6403320126738813
>10	0.7257960805691633	8.6639463146113
>50	0.08970733330095014	3.9135340565373093
>100	0.07734453095029904	9.421310604440055
>500	0.004892759880711539	1.9549008111513864
>1k	7.372651875041375E-4	0.7273493785552514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2979	0.12512711175627808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.200305866273182E-5
2	0.0	0.0	0.0	0.0	4.200305866273182E-5
3	0.0	0.0	0.0	4.200305866273182E-5	4.200305866273182E-5
4	0.0	0.0	0.0	4.200305866273182E-5	4.200305866273182E-5
5	0.0	4.200305866273182E-5	0.0	4.200305866273182E-5	4.200305866273182E-5
6	0.0	4.200305866273182E-5	0.0	4.200305866273182E-5	4.200305866273182E-5
7	0.0	4.200305866273182E-5	0.0	4.200305866273182E-5	4.200305866273182E-5
8	0.0	4.200305866273182E-5	0.0	4.200305866273182E-5	4.200305866273182E-5
9	0.0	4.200305866273182E-5	0.0	1.2600917598819546E-4	4.200305866273182E-5
10	0.0	4.200305866273182E-5	0.0	2.520183519763909E-4	4.200305866273182E-5
11	0.0	8.400611732546364E-5	0.0	2.520183519763909E-4	4.200305866273182E-5
12	0.0	8.400611732546364E-5	0.0	2.9402141063912274E-4	4.200305866273182E-5
13	0.0	8.400611732546364E-5	0.0	2.9402141063912274E-4	4.200305866273182E-5
14	0.0	8.400611732546364E-5	0.0	3.780275279645864E-4	4.200305866273182E-5
15	0.0	8.400611732546364E-5	0.0	4.200305866273182E-4	4.200305866273182E-5
16	0.0	8.400611732546364E-5	0.0	5.880428212782455E-4	4.200305866273182E-5
17	0.0	8.400611732546364E-5	0.0	7.560550559291728E-4	4.200305866273182E-5
18	0.0	8.400611732546364E-5	0.0	9.660703492428319E-4	4.200305866273182E-5
19	0.0	8.400611732546364E-5	0.0	9.660703492428319E-4	4.200305866273182E-5
20	0.0	8.400611732546364E-5	0.0	9.660703492428319E-4	4.200305866273182E-5
21	0.0	8.400611732546364E-5	0.0	0.0010500764665682955	4.200305866273182E-5
22	4.200305866273182E-5	8.400611732546364E-5	0.0	0.0015961162291838092	4.200305866273182E-5
23	4.200305866273182E-5	8.400611732546364E-5	0.0	0.002100152933136591	4.200305866273182E-5
24	4.200305866273182E-5	8.400611732546364E-5	0.0	0.003444250810344009	4.200305866273182E-5
25	4.200305866273182E-5	8.400611732546364E-5	0.0	0.004410321159586841	4.200305866273182E-5
26	8.400611732546364E-5	8.400611732546364E-5	0.0	0.0070145107966762136	4.200305866273182E-5
27	8.400611732546364E-5	8.400611732546364E-5	0.0	0.025747874960254605	4.200305866273182E-5
28	1.2600917598819546E-4	8.400611732546364E-5	0.0	0.11055205040031015	4.200305866273182E-5
29	1.2600917598819546E-4	1.2600917598819546E-4	0.0	0.234461073455369	4.200305866273182E-5
30	1.2600917598819546E-4	1.2600917598819546E-4	0.0	0.39604684013089836	4.200305866273182E-5
31	1.2600917598819546E-4	1.2600917598819546E-4	0.0	1.0262607323065265	4.200305866273182E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1135	0.0	28.361235	1
CTTATAC	2795	0.0	18.930233	37
GTATCAA	1850	0.0	17.1	2
TAGACGT	90	4.4491753E-5	16.444445	4
TCTTATA	4855	0.0	16.23275	37
ATCTCGC	645	0.0	14.914728	11
ATGCGTT	165	1.6474587E-8	14.575757	36
CGAATTA	115	2.2117E-5	14.478261	15
TCGCCAT	515	0.0	14.368932	13
TCTATAC	250	0.0	14.059999	3
TTAGTAC	145	8.9207424E-7	14.034483	3
GTTCTAG	610	0.0	13.95082	1
AACGCTA	95	0.0012457598	13.631579	33
TTGCGCG	315	0.0	13.5079365	18
CTAGCAC	425	0.0	13.494118	3
TTCCTCG	715	0.0	13.454546	19
ATTAGAC	125	4.8486443E-5	13.32	3
GTATTAT	210	2.044544E-9	13.214285	1
GTATAAC	85	0.009408599	13.058824	1
TACGTTA	100	0.0018334833	12.949999	19
>>END_MODULE