FastQCFastQC Report
Fri 10 Feb 2017
ERR1632055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415954
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45891.1032469936579525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36410.8753371767070398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30210.7262822331315483No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT13750.3305653990585497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.3240742966770364No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT8130.19545430504334613RNA PCR Primer, Index 29 (95% over 23bp)
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT7420.17838510989195921No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6860.1649220827303019No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA6080.14617000918370782No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC5630.1353515052145189No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG5030.12092683325560039No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA4870.11708025406655542No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.11227203008024925No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA4520.10866586209051964No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4510.10842545089120431No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT4450.10698298369531246No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.10626175009736655No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG4270.1026555821076369No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG4270.1026555821076369No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC4170.1002514701144838RNA PCR Primer, Index 29 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC200.001840586237.03
GGTATCA10700.025.5887851
GTACTAT1100.025.2272721
GCCGTCT1550.023.87096836
TACACAT2950.023.830515
CGGTCTA551.8988765E-523.54545416
TACTATA1500.022.22
CCGTCTT1700.021.76470637
TTAGGCA705.0890176E-621.1428574
ATACACA3400.020.6764724
TAGCGCA450.003821766520.55555519
TATAGAG450.003821766520.5555552
GGTTAGA450.003821766520.5555551
TGCGATG555.1355135E-420.18181828
GGCTAAA656.8886045E-519.9230771
TATACAC3850.019.70133
CTAGAGG500.0070275218.53
CCGATAG609.223313E-418.515
GCAAACC500.0070275218.537
TATATCG500.0070275218.55
Produced by FastQC (version 0.11.5)