##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415954
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.190922553936254	33.0	31.0	34.0	30.0	34.0
2	32.36649244868423	34.0	31.0	34.0	31.0	34.0
3	32.429047442746075	34.0	31.0	34.0	30.0	34.0
4	36.01782649042923	37.0	35.0	37.0	35.0	37.0
5	35.765262504988534	37.0	35.0	37.0	35.0	37.0
6	35.806726224534444	37.0	35.0	37.0	35.0	37.0
7	35.786584574255805	37.0	35.0	37.0	35.0	37.0
8	35.77704746197897	37.0	35.0	37.0	35.0	37.0
9	37.46719348774143	39.0	37.0	39.0	35.0	39.0
10	37.328238218649176	39.0	37.0	39.0	34.0	39.0
11	37.441370920822976	39.0	37.0	39.0	35.0	39.0
12	37.378277405674666	39.0	37.0	39.0	35.0	39.0
13	37.45728854632964	39.0	37.0	39.0	35.0	39.0
14	38.62304726003356	40.0	38.0	41.0	35.0	41.0
15	38.624641186285025	40.0	38.0	41.0	35.0	41.0
16	38.59850848891945	40.0	38.0	41.0	35.0	41.0
17	38.61643835616438	40.0	38.0	41.0	35.0	41.0
18	38.658789193035766	40.0	38.0	41.0	35.0	41.0
19	38.71274948672209	40.0	38.0	41.0	35.0	41.0
20	38.684570890050345	40.0	38.0	41.0	35.0	41.0
21	38.66058506469465	40.0	38.0	41.0	35.0	41.0
22	38.59639287036547	40.0	38.0	41.0	35.0	41.0
23	38.559263764743214	40.0	38.0	41.0	35.0	41.0
24	38.50013222615962	40.0	38.0	41.0	34.0	41.0
25	38.43270169297566	40.0	38.0	41.0	34.0	41.0
26	38.291577914865584	40.0	38.0	41.0	34.0	41.0
27	38.19123028027138	40.0	38.0	41.0	34.0	41.0
28	38.11543584146324	40.0	38.0	41.0	34.0	41.0
29	38.025469162455465	40.0	37.0	41.0	34.0	41.0
30	37.8822345740154	40.0	37.0	41.0	33.0	41.0
31	37.722870798213265	39.0	37.0	41.0	33.0	41.0
32	37.57059915279093	39.0	36.0	41.0	33.0	41.0
33	37.47264120551792	39.0	36.0	41.0	33.0	41.0
34	37.35523880044428	39.0	36.0	40.0	33.0	41.0
35	37.29349158801214	39.0	36.0	40.0	33.0	41.0
36	37.18937670992465	39.0	36.0	40.0	32.0	41.0
37	37.06453357823221	39.0	36.0	40.0	32.0	41.0
38	36.98247642768191	39.0	35.0	40.0	32.0	41.0
39	36.872497920443124	39.0	35.0	40.0	31.0	41.0
40	36.735687119248766	39.0	35.0	40.0	31.0	41.0
41	36.60071305961717	39.0	35.0	40.0	31.0	41.0
42	36.46487832789202	39.0	35.0	40.0	30.0	41.0
43	35.61838328276684	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	0.0
14	1.0
15	1.0
16	4.0
17	5.0
18	10.0
19	26.0
20	53.0
21	109.0
22	180.0
23	269.0
24	509.0
25	815.0
26	1334.0
27	1929.0
28	2842.0
29	3998.0
30	5416.0
31	7062.0
32	9145.0
33	12282.0
34	17246.0
35	24544.0
36	40337.0
37	74771.0
38	123982.0
39	89079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86509565961621	21.146328680575255	13.733970583285652	24.254605076522886
2	18.65182207647961	21.12421085023825	34.93968083009179	25.28428624319035
3	18.516470571265092	24.42818196242854	31.867946936440088	25.187400529866284
4	13.422157257773696	18.11113728921948	36.64299417724075	31.82371127576607
5	12.67832500709213	38.106617558672355	35.250292099607165	13.964765334628348
6	29.406617077849955	38.298465695725966	16.7593051154695	15.535612110954578
7	27.28739235588551	31.737403655211878	22.388773758636773	18.586430230265847
8	27.58550224303649	33.29166205878535	19.64712444164499	19.475711256533174
9	26.293292046716704	13.673146549858878	22.295494213302433	37.73806719012198
10	17.973381672011808	27.084966126062014	32.41969063886872	22.521961563057452
11	33.753491972670055	22.993648336114088	22.626300023560297	20.62655966765556
12	20.70132755064262	29.109468835496234	28.10911783514523	22.080085778715915
13	30.44519345889209	19.954850776768584	25.56123994480159	24.038715819537735
14	21.77764848997726	21.84929102737322	28.052140380907503	28.32092010174202
15	25.32179039028354	27.647528332459842	23.830279309731363	23.200401967525256
16	22.96840516018598	26.081489780119917	26.541155993210786	24.408949066483313
17	21.049683378450503	28.312505709765983	27.77710996889079	22.860700942892723
18	21.556951009005804	26.354837313741424	28.360587949629046	23.72762372762373
19	22.92032292032292	26.571207393125203	30.100684210273254	20.40778547627863
20	22.97826201935791	25.700678440404467	29.652076912350882	21.668982627886738
21	23.505724190655698	25.4232439163946	28.934930304793316	22.136101588156382
22	23.401145318953535	26.21828375253033	29.041672877289315	21.338898051226817
23	22.39189910422787	25.611726296657807	29.149857916981205	22.84651668213312
24	21.446842679719392	27.561941945503587	28.229083023603575	22.76213235117345
25	22.109896767431014	26.180058371839195	28.990946114233786	22.71909874649601
26	21.42544608298033	26.8459973939426	29.39435610668487	22.334200416392196
27	21.292258278559647	27.172475802612787	29.081581136375657	22.453684782451905
28	21.107382066286178	26.626982791366355	30.299504272107015	21.96613087024046
29	22.07287344273646	27.147953860282627	29.32319439168754	21.455978305293375
30	22.48830399515331	26.814984349230926	28.88829053212615	21.808421123489616
31	22.02839737086312	26.615202642599904	29.227270323160738	22.129129663376236
32	20.930920245988737	26.84743986113849	29.844646283002447	22.376993609870322
33	20.72584949297278	27.039768820590737	29.858590132562735	22.375791553873743
34	22.057246714780963	25.78818811695524	29.89777715805113	22.256788010212667
35	21.60575448246681	26.105290488852134	30.356722137544057	21.932232891137
36	22.56523557893421	26.192800165402907	28.43727912221063	22.804685133452256
37	21.306682950518567	25.405213076445953	29.57586656216793	23.712237410867548
38	21.483625593214633	24.90587901546806	30.770950633964333	22.839544757352975
39	20.654206955576818	25.28476706558898	30.644975165523107	23.416050813311088
40	20.600595258129506	24.677247964919196	32.48940988667016	22.23274689028114
41	20.014953576597414	24.931122191396163	31.724661861648162	23.32926237035826
42	20.0320227717488	25.398962385263758	31.592916524423377	22.976098318564073
43	19.6661169263909	24.19281939829885	31.802555090226324	24.33850858508393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	148.0
2	89.0
3	220.0
4	351.0
5	351.0
6	547.5
7	744.0
8	788.0
9	832.0
10	1204.5
11	1577.0
12	1577.0
13	2786.5
14	3996.0
15	6290.5
16	8585.0
17	7487.0
18	6389.0
19	6389.0
20	6458.0
21	6527.0
22	4709.0
23	2891.0
24	2980.0
25	3069.0
26	3069.0
27	3867.5
28	4666.0
29	6259.0
30	7852.0
31	10297.0
32	12742.0
33	12742.0
34	16546.0
35	20350.0
36	22011.0
37	23672.0
38	23797.0
39	23922.0
40	23922.0
41	26638.0
42	29354.0
43	30647.5
44	31941.0
45	32512.5
46	33084.0
47	33084.0
48	33516.5
49	33949.0
50	34162.0
51	34375.0
52	32059.0
53	29743.0
54	29743.0
55	28138.0
56	26533.0
57	23297.0
58	20061.0
59	17703.5
60	15346.0
61	15346.0
62	13674.5
63	12003.0
64	10045.0
65	8087.0
66	6514.5
67	4942.0
68	4942.0
69	4014.5
70	3087.0
71	2567.5
72	2048.0
73	1646.5
74	1245.0
75	1245.0
76	985.0
77	725.0
78	578.0
79	431.0
80	330.5
81	230.0
82	230.0
83	197.0
84	164.0
85	124.5
86	85.0
87	60.5
88	36.0
89	36.0
90	28.0
91	20.0
92	11.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	415954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.30073717484209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15529642885501	36.1864783877429
2	10.90901354180022	10.101907376713704
3	3.672903331110411	5.101743954070354
4	1.7426625453854698	3.227462419933359
5	1.0919529825121823	2.5279114025290745
6	0.7361459721581081	2.045046070752671
7	0.5108296494284886	1.6556252537513625
8	0.4423952528594288	1.6386581064033776
9	0.32137699161252914	1.3391992460423827
>10	2.161175619854493	18.669516720726413
>50	0.16684686957405648	5.198461032880895
>100	0.08368332087670642	8.002852793269739
>500	0.0031186330761505503	0.9421774428835938
>1k	0.0025988608967921246	3.3629597923002144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4589	1.1032469936579525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.8753371767070398	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3021	0.7262822331315483	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	1375	0.3305653990585497	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1348	0.3240742966770364	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	813	0.19545430504334613	RNA PCR Primer, Index 29 (95% over 23bp)
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT	742	0.17838510989195921	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	686	0.1649220827303019	No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA	608	0.14617000918370782	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	563	0.1353515052145189	No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG	503	0.12092683325560039	No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA	487	0.11708025406655542	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.11227203008024925	No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA	452	0.10866586209051964	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	451	0.10842545089120431	No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT	445	0.10698298369531246	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.10626175009736655	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	427	0.1026555821076369	No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG	427	0.1026555821076369	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	417	0.1002514701144838	RNA PCR Primer, Index 29 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.404111993153089E-4	0.0
2	0.0	0.0	0.0	2.404111993153089E-4	0.0
3	0.0	0.0	0.0	2.404111993153089E-4	0.0
4	0.0	0.0	0.0	2.404111993153089E-4	0.0
5	0.0	0.0	0.0	4.808223986306178E-4	0.0
6	0.0	0.0	0.0	4.808223986306178E-4	0.0
7	0.0	0.0	0.0	4.808223986306178E-4	0.0
8	0.0	0.0	0.0	4.808223986306178E-4	0.0
9	0.0	0.0	0.0	7.212335979459268E-4	0.0
10	0.0	0.0	0.0	0.0014424671958918535	0.0
11	0.0	0.0	0.0	0.0014424671958918535	0.0
12	0.0	0.0	0.0	0.0014424671958918535	0.0
13	0.0	0.0	0.0	0.0014424671958918535	0.0
14	0.0	0.0	0.0	0.0014424671958918535	0.0
15	0.0	0.0	0.0	0.0014424671958918535	0.0
16	0.0	0.0	0.0	0.0014424671958918535	0.0
17	0.0	0.0	0.0	0.0019232895945224713	0.0
18	0.0	0.0	0.0	0.0019232895945224713	0.0
19	0.0	0.0	0.0	0.00216370079383778	0.0
20	0.0	0.0	0.0	0.00216370079383778	0.0
21	0.0	0.0	0.0	0.0036061679897296337	0.0
22	0.0	0.0	0.0	0.006250691182198032	0.0
23	0.0	0.0	0.0	0.007933569577405195	0.0
24	0.0	0.0	0.0	0.012501382364396064	0.0
25	0.0	0.0	0.0	0.01779042874933286	0.0
26	0.0	0.0	0.0	0.02211783033700842	0.0
27	0.0	0.0	0.0	0.05024594065689956	0.0
28	0.0	0.0	0.0	0.12453300124533001	0.0
29	0.0	0.0	0.0	0.23392009693379556	0.0
30	0.0	0.0	0.0	0.40340999245108833	0.0
31	0.0	0.0	0.0	0.9169283141885881	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGAAC	20	0.0018405862	37.0	3
GGTATCA	1070	0.0	25.588785	1
GTACTAT	110	0.0	25.227272	1
GCCGTCT	155	0.0	23.870968	36
TACACAT	295	0.0	23.83051	5
CGGTCTA	55	1.8988765E-5	23.545454	16
TACTATA	150	0.0	22.2	2
CCGTCTT	170	0.0	21.764706	37
TTAGGCA	70	5.0890176E-6	21.142857	4
ATACACA	340	0.0	20.676472	4
TAGCGCA	45	0.0038217665	20.555555	19
TATAGAG	45	0.0038217665	20.555555	2
GGTTAGA	45	0.0038217665	20.555555	1
TGCGATG	55	5.1355135E-4	20.181818	28
GGCTAAA	65	6.8886045E-5	19.923077	1
TATACAC	385	0.0	19.7013	3
CTAGAGG	50	0.00702752	18.5	3
CCGATAG	60	9.223313E-4	18.5	15
GCAAACC	50	0.00702752	18.5	37
TATATCG	50	0.00702752	18.5	5
>>END_MODULE