FastQCFastQC Report
Fri 10 Feb 2017
ERR1632054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49086
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1060.21594752067799375TruSeq Adapter, Index 8 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA358.748422E-426.428571
GTATCAA358.748422E-426.428572
TATCAAC500.00694163818.53
CAACGCA500.00694163818.56
ATCAACG500.00694163818.54
AACGCAG500.00694163818.57
TCTCTTA2200.015.97727337
TCTTATA855.235341E-415.23529337
GTCTCTT2651.8189894E-1213.26415236
GCCTGTC1257.0045853E-411.8437
CTCTTAT1150.00509846811.2608736
GCTGTCT1656.1704713E-410.09090936
TGTCTCT4053.6379788E-1210.04938237
CTGTCTC5350.09.68224237
CCTGTCT3003.5629619E-68.63333335
Produced by FastQC (version 0.11.5)