##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632054.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49086
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12914069184696	33.0	31.0	34.0	30.0	34.0
2	32.32604001140855	34.0	31.0	34.0	30.0	34.0
3	32.41959010715886	34.0	31.0	34.0	31.0	34.0
4	36.006946990995395	37.0	35.0	37.0	35.0	37.0
5	35.71957380923278	37.0	35.0	37.0	33.0	37.0
6	35.71199527360143	37.0	35.0	37.0	33.0	37.0
7	35.68396284072852	37.0	35.0	37.0	33.0	37.0
8	35.669681782993116	37.0	35.0	37.0	33.0	37.0
9	37.39862689972701	39.0	37.0	39.0	35.0	39.0
10	37.25369759198142	39.0	37.0	39.0	34.0	39.0
11	37.34775699792201	39.0	37.0	39.0	34.0	39.0
12	37.27117711771177	39.0	37.0	39.0	34.0	39.0
13	37.3426027787964	39.0	37.0	39.0	34.0	39.0
14	38.55761316872428	40.0	38.0	41.0	35.0	41.0
15	38.549708674571164	40.0	38.0	41.0	35.0	41.0
16	38.522755979301635	40.0	38.0	41.0	35.0	41.0
17	38.495538442733164	40.0	38.0	41.0	34.0	41.0
18	38.48164446074237	40.0	38.0	41.0	34.0	41.0
19	38.500427820559835	40.0	38.0	41.0	34.0	41.0
20	38.4787719512692	40.0	38.0	41.0	34.0	41.0
21	38.4756549729047	40.0	38.0	41.0	34.0	41.0
22	38.437456708633825	40.0	38.0	41.0	34.0	41.0
23	38.3563337815263	40.0	38.0	41.0	34.0	41.0
24	38.32616224585421	40.0	38.0	41.0	34.0	41.0
25	38.20810414374771	40.0	38.0	41.0	34.0	41.0
26	38.106466202175774	40.0	38.0	41.0	34.0	41.0
27	38.055046245365276	40.0	38.0	41.0	33.0	41.0
28	37.963940838528295	40.0	38.0	41.0	33.0	41.0
29	37.856374526341526	40.0	37.0	41.0	33.0	41.0
30	37.799576253921686	40.0	37.0	41.0	33.0	41.0
31	37.70152385608931	40.0	37.0	41.0	33.0	41.0
32	37.62217332844396	39.0	37.0	41.0	33.0	41.0
33	37.56358228415434	39.0	37.0	41.0	33.0	41.0
34	37.49938882777167	39.0	37.0	40.0	32.0	41.0
35	37.47999429572587	39.0	37.0	40.0	32.0	41.0
36	37.43808825326977	39.0	37.0	40.0	32.0	41.0
37	37.38210487715438	39.0	37.0	40.0	32.0	41.0
38	37.37157234241943	39.0	37.0	40.0	32.0	41.0
39	37.3204579717231	39.0	36.0	40.0	32.0	41.0
40	37.26315038911299	39.0	36.0	40.0	32.0	41.0
41	37.24740251802958	39.0	36.0	40.0	32.0	41.0
42	37.205027910198424	39.0	36.0	40.0	32.0	41.0
43	36.296968585747464	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	1.0
20	3.0
21	15.0
22	25.0
23	30.0
24	63.0
25	83.0
26	145.0
27	214.0
28	309.0
29	413.0
30	595.0
31	777.0
32	1091.0
33	1509.0
34	2085.0
35	3041.0
36	4715.0
37	8503.0
38	15246.0
39	10220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92234038218637	18.606119871246385	12.673674774884896	26.797864971682355
2	20.2970297029703	19.895693273031007	32.92181069958848	26.885466324410217
3	21.11396324817667	21.16693150796561	28.384875524589496	29.334229719268222
4	16.084015808988305	15.189667114859635	32.974778959377424	35.75153811677464
5	16.784826630811228	34.30102269486208	32.59585217781037	16.318298496516316
6	37.31613902130954	33.07052927514974	14.297355661492075	15.315976042048648
7	30.97624577272542	27.863341889744532	20.48853033451493	20.671882003015117
8	27.728883999511062	32.20062747015442	19.08283420934686	20.98765432098765
9	26.93028562115471	13.779896508169335	18.335166850018336	40.95465102065762
10	18.377948906001713	25.286232326936396	32.017275801654236	24.31854296540765
11	37.990465713237995	20.728924744326285	19.32934034144155	21.951269200994172
12	23.291773621806623	24.125005093101905	27.27457930978283	25.308641975308642
13	32.923847940349596	18.382023387523937	21.88811473739967	26.806013934726806
14	24.469298781730025	19.2906327669804	23.06360265656195	33.17646579472762
15	27.184940716293855	25.83832457319806	20.12386423827568	26.85287047223241
16	26.94250906572139	24.281872631707614	22.093876054272094	26.681742248298907
17	25.0539868801695	24.770810414374772	23.55865216151245	26.616550543943283
18	26.18872998410952	23.318257751701097	24.210569205068655	26.282443059120723
19	26.657295359165545	23.46290184574013	24.041478221896266	25.83832457319806
20	26.8671311575602	23.47512529030681	23.2876991402844	26.370044411848593
21	27.105488326610438	23.835716905023833	22.674489671189342	26.384305097176387
22	27.537383367966427	23.550503198467997	22.849692376645073	26.06242105692051
23	26.85490771299352	23.715519700118158	23.218432954406552	26.211139632481768
24	27.081041437477083	23.477162531067922	22.271116000488938	27.17068003096606
25	26.504502302082063	23.58717353216803	23.37530049301226	26.53302367273764
26	26.027787963981584	23.98036099906287	23.556614920751333	26.435236116204212
27	26.01963900093713	23.78071140447378	23.202135028317645	26.99751456627144
28	26.037974167787148	23.69514729250703	23.79293484904046	26.473943690665365
29	25.705903923725703	23.648290755001426	23.919243776229475	26.726561545043392
30	25.952410055820398	23.672737644134784	23.821456219696042	26.553396080348772
31	26.38023061565416	23.76645071914599	23.450678401173448	26.4026402640264
32	25.88110662918144	23.35289084464002	23.986472721346207	26.779529804832336
33	25.26178543780304	23.403821863667847	24.194271278979752	27.140121419549363
34	26.28651754064295	22.468728354316912	24.406144318135517	26.838609786904616
35	25.30049301226419	22.794686876095017	25.30456749378642	26.60025261785438
36	25.41865297640875	22.813022042945036	24.87267245243043	26.895652528215784
37	24.950087601352728	21.576416900949354	26.178543780303958	27.29495171739396
38	24.13722853766858	21.2891659536324	27.08307867823819	27.490526830460826
39	24.05777614798517	20.832824023143054	27.849081204416738	27.26031862445504
40	23.719594181640385	20.828749541620827	28.74954162082875	26.702114655910037
41	22.973964063072973	21.270830786782383	29.175324939901397	26.579880210243246
42	22.370940797783483	21.124149451982234	29.391272460579394	27.11363728965489
43	21.38695351016583	20.690217169865136	30.25709978405248	27.665729535916554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	3.5
7	5.0
8	4.5
9	4.0
10	4.5
11	5.0
12	5.0
13	8.0
14	11.0
15	20.5
16	30.0
17	43.0
18	56.0
19	56.0
20	67.0
21	78.0
22	93.5
23	109.0
24	151.5
25	194.0
26	194.0
27	223.5
28	253.0
29	407.5
30	562.0
31	709.0
32	856.0
33	856.0
34	1012.0
35	1168.0
36	1343.5
37	1519.0
38	1747.0
39	1975.0
40	1975.0
41	2254.0
42	2533.0
43	2759.5
44	2986.0
45	3262.0
46	3538.0
47	3538.0
48	3592.0
49	3646.0
50	3813.0
51	3980.0
52	4096.0
53	4212.0
54	4212.0
55	4245.5
56	4279.0
57	4082.0
58	3885.0
59	3658.5
60	3432.0
61	3432.0
62	3156.5
63	2881.0
64	2554.0
65	2227.0
66	1923.0
67	1619.0
68	1619.0
69	1379.0
70	1139.0
71	976.0
72	813.0
73	635.5
74	458.0
75	458.0
76	369.0
77	280.0
78	220.5
79	161.0
80	129.0
81	97.0
82	97.0
83	72.0
84	47.0
85	36.5
86	26.0
87	19.0
88	12.0
89	12.0
90	8.0
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	49086.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.38654606201361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.84149984395202	86.67237094079778
2	3.4464309599179637	6.299148433361855
3	0.9184537875072452	2.5180295807358517
4	0.37005662312185117	1.3527278653791304
5	0.16050648713718846	0.7334066740007334
6	0.06687770297382853	0.3667033370003667
7	0.05127290561326854	0.3279957625392169
8	0.044585135315885684	0.32595852177810375
9	0.028980337955325692	0.23835716905023838
>10	0.0691069597396228	0.9493541946787272
>50	0.0	0.0
>100	0.002229256765794284	0.21594752067799375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	106	0.21594752067799375	TruSeq Adapter, Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0020372407611131483	0.0
21	0.0	0.0	0.0	0.0020372407611131483	0.0
22	0.0	0.0	0.0	0.0020372407611131483	0.0
23	0.0	0.0	0.0	0.0020372407611131483	0.0
24	0.0	0.0	0.0	0.0020372407611131483	0.0
25	0.0	0.0	0.0	0.0020372407611131483	0.0
26	0.0	0.0	0.0	0.006111722283339445	0.0
27	0.0	0.0	0.0	0.02444688913335778	0.0
28	0.0	0.0	0.0	0.1181599641445626	0.0
29	0.0	0.0	0.0	0.21391027991688058	0.0
30	0.0	0.0	0.0	0.3605916147170273	0.0
31	0.0	0.0	0.0	0.8210080267285987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	8.748422E-4	26.42857	1
GTATCAA	35	8.748422E-4	26.42857	2
TATCAAC	50	0.006941638	18.5	3
CAACGCA	50	0.006941638	18.5	6
ATCAACG	50	0.006941638	18.5	4
AACGCAG	50	0.006941638	18.5	7
TCTCTTA	220	0.0	15.977273	37
TCTTATA	85	5.235341E-4	15.235293	37
GTCTCTT	265	1.8189894E-12	13.264152	36
GCCTGTC	125	7.0045853E-4	11.84	37
CTCTTAT	115	0.005098468	11.26087	36
GCTGTCT	165	6.1704713E-4	10.090909	36
TGTCTCT	405	3.6379788E-12	10.049382	37
CTGTCTC	535	0.0	9.682242	37
CCTGTCT	300	3.5629619E-6	8.633333	35
>>END_MODULE