FastQCFastQC Report
Fri 10 Feb 2017
ERR1632053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218673
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4620.21127436857773935No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3910.17880579678332487No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3510.16051364365971107No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG2980.1362765407709228No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC2820.12895967952147727No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG2750.12575855272484485No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT2470.1129540455383152TruSeq Adapter, Index 6 (95% over 21bp)
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT2350.10746639960123106No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2350.10746639960123106No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC2310.10563718428886967No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA2210.10106414600796623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT352.3795374E-531.71428534
CTTAAGC250.005486733329.63
TAATACA250.005486733329.64
TAAGACT250.005486733329.64
CTAATAC250.005486733329.63
GCCGTCT405.9184902E-527.7536
TACACAT556.2228355E-726.9090945
ACACATC601.3291483E-624.6666686
GATTATC601.3291483E-624.66666824
TCTTAAG451.3184307E-424.6666662
TGTTCAT551.8935383E-523.5454562
GTACCGG400.00192621723.1256
TTAAGCT400.00192621723.1254
GAATGGT652.6663838E-622.76922833
ATACACA652.6663838E-622.7692284
CGTATGC502.6924163E-422.231
CCTAGGA502.6924163E-422.22
TCGTATG502.6924163E-422.230
TGCCGTC502.6924163E-422.235
GGTATCA753.7121754E-722.1999991
Produced by FastQC (version 0.11.5)