##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632053.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	218673
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37233677683116	33.0	31.0	34.0	31.0	34.0
2	32.56210871941209	34.0	31.0	34.0	31.0	34.0
3	32.63745409812825	34.0	31.0	34.0	31.0	34.0
4	36.163975433638356	37.0	35.0	37.0	35.0	37.0
5	35.95249985137625	37.0	35.0	37.0	35.0	37.0
6	35.972749264884094	37.0	35.0	37.0	35.0	37.0
7	35.956089686426765	37.0	35.0	37.0	35.0	37.0
8	35.93385100126673	37.0	35.0	37.0	35.0	37.0
9	37.66608131776671	39.0	37.0	39.0	35.0	39.0
10	37.5896932863225	39.0	37.0	39.0	35.0	39.0
11	37.67515422571602	39.0	37.0	39.0	35.0	39.0
12	37.63191614877008	39.0	37.0	39.0	35.0	39.0
13	37.65843062472276	39.0	37.0	39.0	35.0	39.0
14	38.97381020976527	40.0	38.0	41.0	36.0	41.0
15	38.961627635784936	40.0	38.0	41.0	36.0	41.0
16	38.938538365504655	40.0	38.0	41.0	36.0	41.0
17	38.91693533266567	40.0	38.0	41.0	36.0	41.0
18	38.908767886295976	40.0	38.0	41.0	36.0	41.0
19	38.96663968574081	40.0	38.0	41.0	36.0	41.0
20	38.93311016906522	40.0	38.0	41.0	36.0	41.0
21	38.90104402463953	40.0	38.0	41.0	36.0	41.0
22	38.87704471974135	40.0	38.0	41.0	35.0	41.0
23	38.819991494148795	40.0	38.0	41.0	35.0	41.0
24	38.77375807712886	40.0	38.0	41.0	35.0	41.0
25	38.71081477823051	40.0	38.0	41.0	35.0	41.0
26	38.614227636699546	40.0	38.0	41.0	35.0	41.0
27	38.53153795850425	40.0	38.0	41.0	34.0	41.0
28	38.47706849954041	40.0	38.0	41.0	34.0	41.0
29	38.40893022915495	40.0	38.0	41.0	34.0	41.0
30	38.35051423815469	40.0	38.0	41.0	34.0	41.0
31	38.27528775843383	40.0	38.0	41.0	34.0	41.0
32	38.18153590063702	40.0	38.0	41.0	34.0	41.0
33	38.12264888669383	40.0	38.0	41.0	34.0	41.0
34	38.0644889858373	40.0	38.0	41.0	33.0	41.0
35	38.04261157070146	40.0	38.0	41.0	33.0	41.0
36	38.01529224001134	40.0	38.0	41.0	33.0	41.0
37	37.96980880126947	40.0	37.0	41.0	33.0	41.0
38	37.910299854120076	40.0	37.0	41.0	33.0	41.0
39	37.872581434379185	40.0	37.0	41.0	33.0	41.0
40	37.826723006498284	40.0	37.0	41.0	33.0	41.0
41	37.81583460235146	40.0	37.0	41.0	33.0	41.0
42	37.77968930777919	40.0	37.0	41.0	33.0	41.0
43	36.89966754011698	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	7.0
19	14.0
20	23.0
21	40.0
22	65.0
23	115.0
24	205.0
25	308.0
26	468.0
27	696.0
28	1055.0
29	1463.0
30	2032.0
31	2761.0
32	3727.0
33	4919.0
34	7074.0
35	10516.0
36	16918.0
37	30238.0
38	68065.0
39	67961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81952047120586	18.29764076955088	13.197788478687356	26.6850502805559
2	19.070484239023564	20.794976974752256	34.18163193444093	25.952906851783258
3	20.058260507698712	21.673000324685717	30.59911374518116	27.669625422434414
4	16.074686861203716	15.945269877854148	32.5582033447202	35.421839916221934
5	15.854266416064169	35.38937134442752	33.20437365381186	15.55198858569645
6	35.63448619628395	33.5432357904268	15.315105202745652	15.507172810543596
7	29.733894902434226	29.78831405797698	20.250328115496654	20.227462924092137
8	27.061411331074254	33.40192890754688	19.837382758731074	19.69927700264779
9	26.65075249344912	14.68722704677761	18.582083750623077	40.079936709150196
10	17.343247680326332	26.73809752461438	32.42741444988636	23.49124034517293
11	36.73338729518505	20.860371422169173	20.962350175833322	21.443891106812455
12	22.544621421025916	24.571391987122322	27.41079145573528	25.473195136116484
13	31.239339104507646	18.667142262647882	23.621114632350587	26.47240400049389
14	23.67553378789334	19.920612055443517	23.239265935895148	33.164588220767996
15	26.538713055566987	26.928793220928053	20.979727721300755	25.5527660022042
16	26.664471608291834	25.180063382310575	22.249203148079552	25.906261861318043
17	25.303078112066874	25.6808110740695	23.44962569681671	25.566485117046916
18	24.506912147361586	24.31255802042319	25.101864427707127	26.0786654045081
19	26.150919409346372	24.569562771809963	24.694864020706717	24.584653798136944
20	26.416612933466865	23.877204776081182	24.713613477658424	24.992568812793532
21	26.10473172270925	24.902022654831644	23.203596237304104	25.789649385155005
22	26.206710476373395	24.158903934184835	23.489868433688656	26.14451715575311
23	25.553680609860386	24.82976864999337	23.61608429024159	26.000466449904653
24	26.985041591783165	24.279174840972594	23.42447398627174	25.3113095809725
25	26.412039895185963	23.601907871570795	24.024456608726272	25.961595624516974
26	26.27530605058695	23.645809039067466	23.77065298413613	26.30823192620945
27	25.70504817695829	23.935282362248657	23.95357451537227	26.40609494542079
28	25.02412277693177	24.56361782204479	24.30112542472093	26.111133976302515
29	25.267408413475827	24.826567523196736	24.191372505979245	25.71465155734819
30	25.21253195410499	25.439811956665892	23.6110539481326	25.73660214109652
31	25.806569626794346	24.43237162338286	24.229786027538836	25.53127272228396
32	24.657365106803308	24.47490087939526	24.501424501424502	26.36630951237693
33	24.98159352091936	23.89458232154861	24.80736076241694	26.31646339511508
34	25.421062499714186	23.807694594211448	24.623067319696535	26.148175586377832
35	25.15171054496897	23.830102481787875	24.745167441796657	26.273019531446494
36	24.940893480219323	23.673704572580977	25.257805033085933	26.127596914113767
37	25.520297430409787	21.91171292294888	25.676238035788597	26.891751610852733
38	24.16301966863765	22.679068746484475	26.968578653971914	26.189332930905962
39	24.12826457770278	22.124359203010886	27.560787111348912	26.186589107937426
40	23.509532498296544	21.619953080627237	28.92812555733904	25.942388863737175
41	22.738518244136223	22.000887169426495	29.09687066990438	26.163723916532906
42	22.997809514663448	21.17362454441106	29.226744957082033	26.601820983843457
43	21.814764511393726	21.39953263548769	29.81300846469386	26.972694388424724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	5.0
4	9.0
5	9.0
6	11.0
7	13.0
8	15.5
9	18.0
10	29.5
11	41.0
12	41.0
13	66.5
14	92.0
15	184.0
16	276.0
17	315.0
18	354.0
19	354.0
20	445.5
21	537.0
22	750.5
23	964.0
24	1185.0
25	1406.0
26	1406.0
27	1708.5
28	2011.0
29	2914.5
30	3818.0
31	4247.0
32	4676.0
33	4676.0
34	5383.5
35	6091.0
36	6763.5
37	7436.0
38	8905.0
39	10374.0
40	10374.0
41	11812.5
42	13251.0
43	12906.5
44	12562.0
45	13724.0
46	14886.0
47	14886.0
48	15897.5
49	16909.0
50	18225.5
51	19542.0
52	20661.5
53	21781.0
54	21781.0
55	19207.0
56	16633.0
57	15524.5
58	14416.0
59	13114.0
60	11812.0
61	11812.0
62	11092.0
63	10372.0
64	9340.5
65	8309.0
66	7335.0
67	6361.0
68	6361.0
69	5463.5
70	4566.0
71	3984.5
72	3403.0
73	2841.5
74	2280.0
75	2280.0
76	1856.0
77	1432.0
78	1168.5
79	905.0
80	727.5
81	550.0
82	550.0
83	438.0
84	326.0
85	235.0
86	144.0
87	108.0
88	72.0
89	72.0
90	51.5
91	31.0
92	18.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	218673.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.93895640138869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9293830641885	52.703954578853995
2	6.515544820748163	7.81069913884236
3	1.6944781151326989	3.0469574959813834
4	0.8746681948377797	2.0970679518425213
5	0.5381851101624051	1.6129126926950486
6	0.40899558555335835	1.4708861142505911
7	0.2735973335272087	1.1479397052076494
8	0.19617544032069306	0.94068409315242
9	0.1743457793341129	0.9405093659646174
>10	1.1578977923553964	13.91978141156355
>50	0.15451751949369072	6.286026957305782
>100	0.08221124434600212	8.022580494340104
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	462	0.21127436857773935	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	391	0.17880579678332487	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	351	0.16051364365971107	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	298	0.1362765407709228	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	282	0.12895967952147727	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	275	0.12575855272484485	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	247	0.1129540455383152	TruSeq Adapter, Index 6 (95% over 21bp)
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	235	0.10746639960123106	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	235	0.10746639960123106	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	231	0.10563718428886967	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	221	0.10106414600796623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.5730382809034493E-4	0.0
9	0.0	0.0	0.0	4.5730382809034493E-4	0.0
10	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
11	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
12	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
13	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
14	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
15	0.0	4.5730382809034493E-4	0.0	4.5730382809034493E-4	0.0
16	0.0	4.5730382809034493E-4	0.0	9.146076561806899E-4	0.0
17	0.0	4.5730382809034493E-4	0.0	9.146076561806899E-4	0.0
18	0.0	4.5730382809034493E-4	0.0	9.146076561806899E-4	0.0
19	0.0	9.146076561806899E-4	0.0	9.146076561806899E-4	0.0
20	0.0	9.146076561806899E-4	0.0	0.001371911484271035	0.0
21	0.0	9.146076561806899E-4	0.0	0.001371911484271035	0.0
22	0.0	9.146076561806899E-4	0.0	0.0018292153123613797	0.0
23	0.0	9.146076561806899E-4	0.0	0.0018292153123613797	0.0
24	0.0	9.146076561806899E-4	0.0	0.004115734452813104	0.0
25	0.0	9.146076561806899E-4	0.0	0.005944949765174484	0.0
26	0.0	9.146076561806899E-4	0.0	0.009603380389897245	0.0
27	0.0	9.146076561806899E-4	0.0	0.034755090934866215	0.0
28	0.0	9.146076561806899E-4	0.0	0.10929561491359244	0.0
29	0.0	9.146076561806899E-4	0.0	0.21813392599909454	0.0
30	0.0	9.146076561806899E-4	0.0	0.35669698591046906	0.0
31	0.0	9.146076561806899E-4	0.0	0.7618681775985147	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGT	35	2.3795374E-5	31.714285	34
CTTAAGC	25	0.0054867333	29.6	3
TAATACA	25	0.0054867333	29.6	4
TAAGACT	25	0.0054867333	29.6	4
CTAATAC	25	0.0054867333	29.6	3
GCCGTCT	40	5.9184902E-5	27.75	36
TACACAT	55	6.2228355E-7	26.909094	5
ACACATC	60	1.3291483E-6	24.666668	6
GATTATC	60	1.3291483E-6	24.666668	24
TCTTAAG	45	1.3184307E-4	24.666666	2
TGTTCAT	55	1.8935383E-5	23.545456	2
GTACCGG	40	0.001926217	23.125	6
TTAAGCT	40	0.001926217	23.125	4
GAATGGT	65	2.6663838E-6	22.769228	33
ATACACA	65	2.6663838E-6	22.769228	4
CGTATGC	50	2.6924163E-4	22.2	31
CCTAGGA	50	2.6924163E-4	22.2	2
TCGTATG	50	2.6924163E-4	22.2	30
TGCCGTC	50	2.6924163E-4	22.2	35
GGTATCA	75	3.7121754E-7	22.199999	1
>>END_MODULE