FastQCFastQC Report
Fri 10 Feb 2017
ERR1632052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489534
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG352.3855275E-531.7142871
GGTATCA1550.028.6451631
TGCGCAA405.9332684E-527.75000210
CTAGAAC551.8997669E-523.5454543
CTCACCG400.001929611723.12500229
ATACCGT400.001929611723.1250026
ACTATTC502.6990325E-422.28
GTGTAAG502.6990325E-422.21
TCAGTAC603.7206624E-521.5833343
TCTAGCG450.00382271820.55555528
TATCTAG450.00382271820.5555551
TTGCGCA450.00382271820.5555559
TATACAG450.00382271820.5555555
TAGTACA450.00382271820.5555554
TCGTTTA555.137298E-420.18181830
TACATTG555.137298E-420.1818185
GTTATTC555.137298E-420.1818183
AGACCAT851.2423225E-619.5882346
CTAGATA500.007029254518.53
GCGCGTT609.226491E-418.525
Produced by FastQC (version 0.11.5)