##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489534
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.433175223784254	33.0	31.0	34.0	31.0	34.0
2	32.616774320067655	34.0	31.0	34.0	31.0	34.0
3	32.707883415656525	34.0	31.0	34.0	31.0	34.0
4	36.20479272124102	37.0	37.0	37.0	35.0	37.0
5	36.01018519653385	37.0	35.0	37.0	35.0	37.0
6	36.02630869357389	37.0	35.0	37.0	35.0	37.0
7	35.99982432272324	37.0	35.0	37.0	35.0	37.0
8	35.98304101451585	37.0	35.0	37.0	35.0	37.0
9	37.72937324067378	39.0	37.0	39.0	35.0	39.0
10	37.64973423704993	39.0	37.0	39.0	35.0	39.0
11	37.73643710140664	39.0	37.0	39.0	35.0	39.0
12	37.679252105063185	39.0	37.0	39.0	35.0	39.0
13	37.715684712399955	39.0	37.0	39.0	35.0	39.0
14	39.03530908986914	40.0	38.0	41.0	36.0	41.0
15	39.0265579101758	40.0	38.0	41.0	36.0	41.0
16	39.01611532600391	40.0	38.0	41.0	36.0	41.0
17	38.99166350039017	40.0	38.0	41.0	36.0	41.0
18	38.98054884849673	40.0	38.0	41.0	36.0	41.0
19	39.002676014331996	40.0	38.0	41.0	36.0	41.0
20	38.97834675426017	40.0	38.0	41.0	36.0	41.0
21	38.937119791475155	40.0	38.0	41.0	36.0	41.0
22	38.902552223134656	40.0	38.0	41.0	35.0	41.0
23	38.852196170235366	40.0	38.0	41.0	35.0	41.0
24	38.79720101157427	40.0	38.0	41.0	35.0	41.0
25	38.73335253526823	40.0	38.0	41.0	35.0	41.0
26	38.63105933397885	40.0	38.0	41.0	35.0	41.0
27	38.53818938010434	40.0	38.0	41.0	34.0	41.0
28	38.47446346934023	40.0	38.0	41.0	34.0	41.0
29	38.40545294096018	40.0	38.0	41.0	34.0	41.0
30	38.322837637426616	40.0	38.0	41.0	34.0	41.0
31	38.23823881487292	40.0	38.0	41.0	34.0	41.0
32	38.14394301519404	40.0	38.0	41.0	34.0	41.0
33	38.05351007284479	40.0	38.0	41.0	33.0	41.0
34	37.98424624234476	40.0	37.0	41.0	33.0	41.0
35	37.95042836656902	40.0	37.0	41.0	33.0	41.0
36	37.91126459040639	40.0	37.0	41.0	33.0	41.0
37	37.84568385444116	40.0	37.0	41.0	33.0	41.0
38	37.799313224413424	40.0	37.0	41.0	33.0	41.0
39	37.75223784251962	40.0	37.0	41.0	33.0	41.0
40	37.68761107502237	40.0	37.0	41.0	33.0	41.0
41	37.6229516233806	40.0	37.0	41.0	33.0	41.0
42	37.57332279269673	40.0	36.0	41.0	33.0	41.0
43	36.66102048070206	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	8.0
18	11.0
19	19.0
20	38.0
21	89.0
22	158.0
23	254.0
24	465.0
25	794.0
26	1134.0
27	1727.0
28	2442.0
29	3292.0
30	4614.0
31	6126.0
32	8408.0
33	11484.0
34	16211.0
35	23876.0
36	37664.0
37	66324.0
38	146764.0
39	157629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67704796806759	17.08727075136763	11.679066213991266	27.556615066573514
2	20.201048343935252	19.25606801570473	31.707297143814323	28.835586496545695
3	20.627576429829183	20.14630240187607	28.75591889429539	30.47020227399935
4	15.91860830912664	14.622477703285163	32.20389186450788	37.255022123080316
5	17.311974244894124	33.55885393047265	32.490082404899354	16.639089419733867
6	37.947721710851546	32.4051036291657	14.486634227653239	15.160540432329523
7	32.29949298720825	28.203556852026622	19.358614519114095	20.13833564165104
8	29.901293883570908	30.78478716493645	18.564798359255946	20.749120592236697
9	28.073024549878046	13.005429653507214	17.741975021142554	41.179570775472186
10	18.34785735005127	24.841992588870234	31.08548129445554	25.724668766622948
11	39.91877990088533	19.283236710831115	19.31857644208574	21.479406946197813
12	24.379103392205646	22.856226533805618	26.845939199320167	25.91873087466856
13	33.336397471881426	17.229446779998938	21.714324234884604	27.71983151323504
14	24.9030710839288	17.958916030347226	22.26832048437902	34.869692401344956
15	27.791736631163516	24.80665285761561	19.77799294839582	27.623617562825054
16	27.79439221790519	23.35220842678956	21.458979355877222	27.394419999428028
17	26.71070855139786	23.36712056772359	22.897081714446802	27.025089166431748
18	26.984846813500184	22.366781469724266	23.458227620553423	27.190144096222124
19	27.53904733889781	22.742649131623136	22.996972631114488	26.721330898364563
20	27.841988503352166	22.5167199826774	22.683817671499835	26.95747384247059
21	28.3549252963022	22.683204843790218	22.159849979776684	26.8020198801309
22	28.62640797166285	22.466263834585547	22.04586402578779	26.861464167963817
23	27.767836350488423	22.36126602033771	22.202747919449926	27.668149709723945
24	27.65834446637006	22.40273402868851	22.1048997618143	27.834021743127135
25	27.728002549363275	21.926771174218747	22.52652522603129	27.818701050386696
26	27.3208806742739	22.31183125176188	22.782278656845083	27.58500941711914
27	27.328847434498933	22.40661527084942	22.654810493244597	27.609726801407053
28	27.30801129237193	22.40212120097889	22.723242920818574	27.566624585830606
29	26.96380639546998	22.788202658038053	23.104421756200797	27.143569190291174
30	26.84287506077208	23.008616357597226	22.749798788235342	27.398709793395355
31	27.36194013081829	22.88216957351277	22.436439552717484	27.319450742951464
32	26.670261922563093	22.83375618445297	22.856430809708826	27.639551083275116
33	26.492541886773953	22.530406468192197	23.501125560226665	27.475926084807185
34	27.05491344829981	22.108576728072002	23.450260860328395	27.386248963299792
35	26.591615699828818	22.331033186663234	24.03081297723958	27.04653813626837
36	26.36589082678629	22.508140394742757	23.656783798469565	27.469184980001387
37	26.276622257085307	21.66407236269595	24.391768498204414	27.667536882014325
38	25.567172045251198	21.881830475513446	24.963945303084156	27.587052176151193
39	25.477699199647013	21.323544432051705	25.744483529233925	27.454272839067357
40	25.210914870060098	21.119472804749005	26.290513018503308	27.379099306687582
41	24.45591113181107	20.905391658189217	27.237944657572307	27.40075255242741
42	24.184428456450423	20.947063942443222	27.687351644625295	27.181155956481064
43	23.689672218885715	19.98717147327867	28.24992748205436	28.073228825781253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	14.0
4	24.0
5	24.0
6	29.5
7	35.0
8	38.0
9	41.0
10	60.0
11	79.0
12	79.0
13	123.0
14	167.0
15	299.5
16	432.0
17	466.0
18	500.0
19	500.0
20	640.5
21	781.0
22	950.5
23	1120.0
24	1453.0
25	1786.0
26	1786.0
27	2322.0
28	2858.0
29	3660.0
30	4462.0
31	5438.0
32	6414.0
33	6414.0
34	8021.5
35	9629.0
36	11455.0
37	13281.0
38	15130.5
39	16980.0
40	16980.0
41	19157.0
42	21334.0
43	23569.0
44	25804.0
45	27718.5
46	29633.0
47	29633.0
48	31360.5
49	33088.0
50	34982.0
51	36876.0
52	38047.5
53	39219.0
54	39219.0
55	39359.5
56	39500.0
57	39476.0
58	39452.0
59	38636.5
60	37821.0
61	37821.0
62	35807.5
63	33794.0
64	30637.5
65	27481.0
66	24440.5
67	21400.0
68	21400.0
69	18596.0
70	15792.0
71	13506.5
72	11221.0
73	9185.0
74	7149.0
75	7149.0
76	5822.5
77	4496.0
78	3679.0
79	2862.0
80	2409.0
81	1956.0
82	1956.0
83	1482.5
84	1009.0
85	791.5
86	574.0
87	433.0
88	292.0
89	292.0
90	216.0
91	140.0
92	86.5
93	33.0
94	21.0
95	9.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	489534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.19908172228945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.1091287634466	70.46451124097062
2	6.092642814030306	9.528781466381508
3	1.512650006625398	3.548635244559635
4	0.6669947581589998	2.086335104064572
5	0.4032561074713696	1.576712865158298
6	0.25175162071565604	1.1812047337237441
7	0.18646135848936024	1.0206774907391014
8	0.132120308806696	0.8265349460439151
9	0.09438123285679917	0.664247316709734
>10	0.5218828531832166	7.283166988020656
>50	0.023245325464387248	1.2301558024415677
>100	0.0054848507511821715	0.5890368011866886
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.0855180641181205E-4	0.0
10	0.0	0.0	0.0	6.12827709617718E-4	0.0
11	0.0	0.0	0.0	6.12827709617718E-4	0.0
12	0.0	0.0	0.0	6.12827709617718E-4	0.0
13	0.0	0.0	0.0	6.12827709617718E-4	0.0
14	0.0	0.0	0.0	6.12827709617718E-4	0.0
15	0.0	0.0	0.0	6.12827709617718E-4	0.0
16	0.0	0.0	0.0	0.001225655419235436	2.0427590320590603E-4
17	0.0	0.0	0.0	0.0018384831288531542	2.0427590320590603E-4
18	0.0	0.0	0.0	0.0018384831288531542	2.0427590320590603E-4
19	0.0	0.0	0.0	0.00204275903205906	2.0427590320590603E-4
20	0.0	0.0	0.0	0.002451310838470872	2.0427590320590603E-4
21	0.0	0.0	0.0	0.002451310838470872	2.0427590320590603E-4
22	0.0	0.0	0.0	0.0032684144512944964	2.0427590320590603E-4
23	0.0	0.0	0.0	0.004494069870529933	2.0427590320590603E-4
24	0.0	0.0	0.0	0.006536828902588993	2.0427590320590603E-4
25	0.0	0.0	0.0	0.007966760225030334	2.0427590320590603E-4
26	0.0	0.0	0.0	0.0102137951602953	2.0427590320590603E-4
27	0.0	0.0	0.0	0.02614731561035597	2.0427590320590603E-4
28	0.0	0.0	0.0	0.08416167212083328	2.0427590320590603E-4
29	0.0	0.0	0.0	0.18507396830455086	2.0427590320590603E-4
30	0.0	0.0	0.0	0.30294116445435865	2.0427590320590603E-4
31	0.0	0.0	0.0	0.6553170974845465	2.0427590320590603E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	35	2.3855275E-5	31.714287	1
GGTATCA	155	0.0	28.645163	1
TGCGCAA	40	5.9332684E-5	27.750002	10
CTAGAAC	55	1.8997669E-5	23.545454	3
CTCACCG	40	0.0019296117	23.125002	29
ATACCGT	40	0.0019296117	23.125002	6
ACTATTC	50	2.6990325E-4	22.2	8
GTGTAAG	50	2.6990325E-4	22.2	1
TCAGTAC	60	3.7206624E-5	21.583334	3
TCTAGCG	45	0.003822718	20.555555	28
TATCTAG	45	0.003822718	20.555555	1
TTGCGCA	45	0.003822718	20.555555	9
TATACAG	45	0.003822718	20.555555	5
TAGTACA	45	0.003822718	20.555555	4
TCGTTTA	55	5.137298E-4	20.181818	30
TACATTG	55	5.137298E-4	20.181818	5
GTTATTC	55	5.137298E-4	20.181818	3
AGACCAT	85	1.2423225E-6	19.588234	6
CTAGATA	50	0.0070292545	18.5	3
GCGCGTT	60	9.226491E-4	18.5	25
>>END_MODULE