FastQCFastQC Report
Fri 10 Feb 2017
ERR1632051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408264
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.14916818529186998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4390.10752846197558442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA200.00184054637.08
TAGATAA303.59545E-430.8333324
CGCCGTT303.59545E-430.83333225
GTATAAT250.005492244429.63
GGTATCA2850.025.315791
TTTCGGA451.3211713E-424.66666630
CTAGATA400.00192906623.1250023
CCGATCG400.00192906623.12500218
AGGACCG903.810783E-922.611115
CCTTAGG502.6979687E-422.21
GTTTTCG502.6979687E-422.228
TTTTCGG502.6979687E-422.229
TAACACT603.7187157E-521.5833324
CTATCCT450.003821646320.5555554
CCTTTAG450.003821646320.5555553
TACTATG555.135289E-420.1818182
CTTATAC3900.019.92307737
GTATCAA3650.019.7671222
GATCGCA609.222916E-418.520
TTTCCGA500.00702730418.533
Produced by FastQC (version 0.11.5)