##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408264
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19167989340231	33.0	31.0	34.0	30.0	34.0
2	32.38326181098505	34.0	31.0	34.0	31.0	34.0
3	32.47344610350166	34.0	31.0	34.0	31.0	34.0
4	36.062741265455685	37.0	35.0	37.0	35.0	37.0
5	35.77937070131092	37.0	35.0	37.0	35.0	37.0
6	35.77925557972292	37.0	35.0	37.0	35.0	37.0
7	35.750646640409144	37.0	35.0	37.0	35.0	37.0
8	35.72714469069034	37.0	35.0	37.0	33.0	37.0
9	37.439443595320675	39.0	37.0	39.0	35.0	39.0
10	37.33007808672819	39.0	37.0	39.0	34.0	39.0
11	37.424139772304194	39.0	37.0	39.0	35.0	39.0
12	37.36976809123508	39.0	37.0	39.0	35.0	39.0
13	37.41033008053613	39.0	37.0	39.0	35.0	39.0
14	38.639674328375754	40.0	38.0	41.0	35.0	41.0
15	38.62673417200635	40.0	38.0	41.0	35.0	41.0
16	38.61833519487391	40.0	38.0	41.0	35.0	41.0
17	38.596378813708775	40.0	38.0	41.0	35.0	41.0
18	38.58757813571611	40.0	38.0	41.0	35.0	41.0
19	38.616838614230005	40.0	38.0	41.0	35.0	41.0
20	38.589128603060765	40.0	38.0	41.0	35.0	41.0
21	38.5585234064233	40.0	38.0	41.0	34.0	41.0
22	38.511548899731544	40.0	38.0	41.0	34.0	41.0
23	38.453461485705326	40.0	38.0	41.0	34.0	41.0
24	38.39944496698215	40.0	38.0	41.0	34.0	41.0
25	38.32415544843533	40.0	38.0	41.0	34.0	41.0
26	38.220749319068055	40.0	38.0	41.0	34.0	41.0
27	38.13466286520487	40.0	38.0	41.0	34.0	41.0
28	38.04418219583407	40.0	38.0	41.0	33.0	41.0
29	37.966257127740874	40.0	37.0	41.0	33.0	41.0
30	37.887119608880525	40.0	37.0	41.0	33.0	41.0
31	37.80290694256657	40.0	37.0	41.0	33.0	41.0
32	37.6898232516215	40.0	37.0	41.0	33.0	41.0
33	37.60033458742382	39.0	37.0	41.0	33.0	41.0
34	37.516521172574606	39.0	37.0	41.0	32.0	41.0
35	37.48764035036153	39.0	37.0	41.0	32.0	41.0
36	37.46581623655282	39.0	37.0	41.0	32.0	41.0
37	37.40113994866067	39.0	36.0	40.0	32.0	41.0
38	37.36332373170301	39.0	36.0	40.0	32.0	41.0
39	37.30069023964885	39.0	36.0	40.0	32.0	41.0
40	37.23063018047146	39.0	36.0	40.0	32.0	41.0
41	37.17760321752591	39.0	36.0	40.0	32.0	41.0
42	37.12535516234593	39.0	36.0	40.0	32.0	41.0
43	36.202004095389256	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	3.0
18	19.0
19	30.0
20	61.0
21	95.0
22	175.0
23	286.0
24	474.0
25	822.0
26	1170.0
27	1672.0
28	2498.0
29	3485.0
30	4735.0
31	6482.0
32	8455.0
33	12067.0
34	16684.0
35	24658.0
36	38625.0
37	67960.0
38	126260.0
39	91544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.719416847921934	18.297719122920462	12.381939137420884	26.60092489173672
2	19.52805047714224	20.252091783747773	33.106274371485114	27.113583367624873
3	20.100719142515626	21.24262731957753	29.560774400877865	29.09587913702898
4	15.644533929026316	15.451521564477888	33.2733231438481	35.630621362647695
5	16.467040934297415	34.39857543158348	32.94779848333431	16.186585150784786
6	36.12515431191582	33.608400446769735	15.077988752375916	15.18845648893853
7	30.79575960652911	29.446387631532538	20.113211059510512	19.64464170242784
8	28.709609468383203	31.92346128975369	19.24147120490663	20.125458036956477
9	27.36832049850097	13.726412321439069	18.567152626731723	40.33811455332824
10	17.689289283404854	25.69097446750142	31.97514353457567	24.64459271451806
11	38.31956773068407	20.148727294103814	20.380685046930417	21.1510199282817
12	23.32240902945153	24.069719593204397	27.59439970215351	25.013471675190562
13	32.25339486214802	18.323192836008072	22.696343542413732	26.72706875943017
14	23.819636313757762	19.356347853349792	23.43263182646523	33.39138400642722
15	26.69424685987498	26.00768130425411	20.817167323104655	26.48090451276625
16	26.627378363020004	24.676924734975408	22.52929477005075	26.166402131953838
17	25.307399133893753	24.782003801461798	24.027834930339193	25.882762134305253
18	25.615533086434265	23.785589716457977	24.73522034761821	25.863656849489548
19	26.258989281445338	24.077802598318733	24.39940822604981	25.263799894186118
20	26.442204064036996	23.65381223913938	24.16328650089158	25.740697195932043
21	27.275978288558385	23.793672721572314	23.48774322497208	25.442605764897223
22	27.27769286540082	23.84951893872592	23.270481845080635	25.602306350792624
23	26.475025963592184	23.65479199733506	23.57445182528952	26.295730213783237
24	26.274910352125097	23.73072325750005	23.597476142887935	26.39689024748692
25	26.246987243548293	23.22026923755217	23.75105324006035	26.781690278839182
26	25.91656379205612	23.655281876432895	24.07951717516117	26.348637156349813
27	26.01747888621088	23.765504673446593	23.856622185644582	26.360394254697937
28	25.800707385417276	23.889199145650853	24.263711715948507	26.046381752983365
29	25.56727999529716	24.160592165853465	24.25709834812768	26.01502949072169
30	25.475427664452415	24.30510649971587	24.016567711088904	26.202898124742813
31	26.04025826426038	24.13070954088531	23.666059216585346	26.16297297826896
32	25.33091333059001	24.062861285834654	24.015587952893224	26.590637430682108
33	25.181255266200303	23.7263143456195	24.864303489898692	26.228126898281506
34	25.84381674602708	23.284198459820114	24.714890365057904	26.1570944290949
35	25.47714224129485	23.276850273352537	25.38210569631415	25.86390178903846
36	25.190807908608154	23.39221680089354	24.96056473262399	26.456410557874317
37	24.91500597652499	22.577547861187856	25.76053729939451	26.74690886289264
38	24.295063978210177	22.4969627495934	26.650647620167344	26.55732565202908
39	24.389855583641957	22.071012874022692	27.118482158603257	26.42064938373209
40	23.97321341093018	21.956381165128448	27.90130895694942	26.16909646699195
41	23.234965610487333	21.62742735092979	28.72895969274783	26.408647345835046
42	22.906011796288674	21.755285795465678	28.92074735955166	26.41795504869398
43	22.204994807281565	21.15053004918386	29.36090372895969	27.28357141457488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	12.0
2	13.0
3	39.5
4	66.0
5	66.0
6	84.5
7	103.0
8	103.5
9	104.0
10	158.0
11	212.0
12	212.0
13	351.0
14	490.0
15	860.0
16	1230.0
17	1208.0
18	1186.0
19	1186.0
20	1481.0
21	1776.0
22	1819.0
23	1862.0
24	2329.5
25	2797.0
26	2797.0
27	3462.5
28	4128.0
29	5109.0
30	6090.0
31	7144.0
32	8198.0
33	8198.0
34	9699.5
35	11201.0
36	12790.5
37	14380.0
38	16014.5
39	17649.0
40	17649.0
41	19203.5
42	20758.0
43	22200.0
44	23642.0
45	24850.0
46	26058.0
47	26058.0
48	27451.5
49	28845.0
50	29826.0
51	30807.0
52	31162.5
53	31518.0
54	31518.0
55	31352.0
56	31186.0
57	30539.0
58	29892.0
59	28827.5
60	27763.0
61	27763.0
62	25691.5
63	23620.0
64	21278.0
65	18936.0
66	16533.5
67	14131.0
68	14131.0
69	12323.5
70	10516.0
71	8874.0
72	7232.0
73	5925.5
74	4619.0
75	4619.0
76	3701.5
77	2784.0
78	2342.5
79	1901.0
80	1570.5
81	1240.0
82	1240.0
83	926.0
84	612.0
85	504.0
86	396.0
87	297.5
88	199.0
89	199.0
90	143.0
91	87.0
92	53.0
93	19.0
94	12.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	408264.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.33939752565708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.94048437734445	73.06103725567282
2	5.7149501621608	9.182713058343097
3	1.4103531906159907	3.3992077689743065
4	0.6165647792292007	1.9813777159525499
5	0.32693053384654136	1.3132701060986285
6	0.206217385766648	0.9940428319085106
7	0.14650675389772477	0.8239185039108042
8	0.10608778173936217	0.6818422775819009
9	0.07896424385181915	0.5709545799411799
>10	0.42823140201094206	6.180014159205623
>50	0.021048739267005203	1.1714761840921821
>100	0.003355596080400528	0.4908926314363509
>500	3.0505418912732076E-4	0.14925292688204578
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	609	0.14916818529186998	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.10752846197558442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.898790978386534E-4	0.0
11	0.0	0.0	0.0	4.898790978386534E-4	0.0
12	0.0	0.0	0.0	7.348186467579801E-4	0.0
13	0.0	0.0	0.0	7.348186467579801E-4	0.0
14	0.0	0.0	0.0	7.348186467579801E-4	0.0
15	0.0	0.0	0.0	7.348186467579801E-4	0.0
16	0.0	0.0	0.0	7.348186467579801E-4	0.0
17	0.0	0.0	0.0	9.797581956773069E-4	0.0
18	0.0	0.0	0.0	9.797581956773069E-4	0.0
19	0.0	0.0	0.0	9.797581956773069E-4	0.0
20	0.0	0.0	0.0	9.797581956773069E-4	0.0
21	0.0	0.0	0.0	0.0012246977445966336	0.0
22	0.0	0.0	0.0	0.0019595163913546138	0.0
23	0.0	0.0	0.0	0.002449395489193267	0.0
24	0.0	0.0	0.0	0.0036740932337899004	0.0
25	0.0	0.0	0.0	0.005878549174063841	0.0
26	0.0	0.0	0.0	0.007838065565418455	0.0
27	0.0	0.0	0.0	0.028168048125722572	0.0
28	0.0	0.0	0.0	0.10116003370368193	0.0
29	0.0	0.0	0.0	0.2236298081633453	0.0
30	0.0	0.0	0.0	0.3843101522544236	0.0
31	0.0	0.0	0.0	0.8352438618149041	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	20	0.001840546	37.0	8
TAGATAA	30	3.59545E-4	30.833332	4
CGCCGTT	30	3.59545E-4	30.833332	25
GTATAAT	25	0.0054922444	29.6	3
GGTATCA	285	0.0	25.31579	1
TTTCGGA	45	1.3211713E-4	24.666666	30
CTAGATA	40	0.001929066	23.125002	3
CCGATCG	40	0.001929066	23.125002	18
AGGACCG	90	3.810783E-9	22.61111	5
CCTTAGG	50	2.6979687E-4	22.2	1
GTTTTCG	50	2.6979687E-4	22.2	28
TTTTCGG	50	2.6979687E-4	22.2	29
TAACACT	60	3.7187157E-5	21.583332	4
CTATCCT	45	0.0038216463	20.555555	4
CCTTTAG	45	0.0038216463	20.555555	3
TACTATG	55	5.135289E-4	20.181818	2
CTTATAC	390	0.0	19.923077	37
GTATCAA	365	0.0	19.767122	2
GATCGCA	60	9.222916E-4	18.5	20
TTTCCGA	50	0.007027304	18.5	33
>>END_MODULE