FastQCFastQC Report
Fri 10 Feb 2017
ERR1632050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086755
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.11860999029220018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.027.112071
GTACTAT1101.7553248E-920.1818181
CTTATAC15450.018.5598737
GTTAACG701.2191195E-418.513
GTATCAA8850.017.5593222
TTGTATG1708.54925E-1116.323534
TTGCGCG1059.342082E-615.85714218
GACTGCG1059.342082E-615.8571427
GACGTAG855.363815E-415.2352941
TAGTCTT855.363815E-415.2352945
TATGCCG750.00410421414.833
TCGTATG750.00410421414.830
TACTATA750.00410421414.82
TCTTATA23350.014.18201337
ATACACA4200.014.09523837
ATTTGCG1203.300033E-513.87500116
GGACGGT950.001244997413.6315796
TTAACTC1102.4561665E-413.4545453
TACTATT1102.4561665E-413.4545452
GTTTAGA1254.8439062E-513.321
Produced by FastQC (version 0.11.5)