##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086755
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71754995376143	34.0	31.0	34.0	31.0	34.0
2	32.89099014957373	34.0	31.0	34.0	31.0	34.0
3	32.980390244351305	34.0	31.0	34.0	31.0	34.0
4	36.38283973848752	37.0	37.0	37.0	35.0	37.0
5	36.25005452010803	37.0	37.0	37.0	35.0	37.0
6	36.27895799881298	37.0	37.0	37.0	35.0	37.0
7	36.252603392669	37.0	37.0	37.0	35.0	37.0
8	36.23834902991015	37.0	37.0	37.0	35.0	37.0
9	38.021503466742736	39.0	38.0	39.0	35.0	39.0
10	37.98308450386702	39.0	38.0	39.0	35.0	39.0
11	38.054861491320494	39.0	38.0	39.0	35.0	39.0
12	38.011768981969254	39.0	38.0	39.0	35.0	39.0
13	38.03593910310972	39.0	38.0	39.0	35.0	39.0
14	39.433286251270985	40.0	39.0	41.0	37.0	41.0
15	39.44220914557559	40.0	39.0	41.0	37.0	41.0
16	39.436554697240865	40.0	39.0	41.0	37.0	41.0
17	39.42440476464336	40.0	39.0	41.0	37.0	41.0
18	39.41808043211211	40.0	39.0	41.0	37.0	41.0
19	39.443804721395345	40.0	39.0	41.0	37.0	41.0
20	39.41441723295499	40.0	39.0	41.0	37.0	41.0
21	39.38846474136305	40.0	39.0	41.0	37.0	41.0
22	39.34769014175228	40.0	39.0	41.0	36.0	41.0
23	39.29512815676026	40.0	39.0	41.0	36.0	41.0
24	39.254117993475994	40.0	39.0	41.0	36.0	41.0
25	39.20134344907546	40.0	39.0	41.0	36.0	41.0
26	39.1156405997672	40.0	39.0	41.0	36.0	41.0
27	39.04139663493612	40.0	39.0	41.0	36.0	41.0
28	38.98712957382298	40.0	39.0	41.0	35.0	41.0
29	38.92077515171313	40.0	38.0	41.0	35.0	41.0
30	38.85312236888719	40.0	38.0	41.0	35.0	41.0
31	38.784266002916944	40.0	38.0	41.0	35.0	41.0
32	38.6987683516524	40.0	38.0	41.0	35.0	41.0
33	38.625862314873174	40.0	38.0	41.0	35.0	41.0
34	38.554782816734225	40.0	38.0	41.0	35.0	41.0
35	38.52981214717209	40.0	38.0	41.0	35.0	41.0
36	38.49898091106091	40.0	38.0	41.0	35.0	41.0
37	38.450730845498754	40.0	38.0	41.0	34.0	41.0
38	38.41437030425441	40.0	38.0	41.0	34.0	41.0
39	38.36672203026441	40.0	38.0	41.0	34.0	41.0
40	38.29920543268722	40.0	38.0	41.0	34.0	41.0
41	38.258975574071435	40.0	38.0	41.0	34.0	41.0
42	38.20251298590759	40.0	38.0	41.0	34.0	41.0
43	37.329613390322564	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	1.0
14	1.0
15	4.0
16	2.0
17	5.0
18	12.0
19	25.0
20	78.0
21	151.0
22	232.0
23	402.0
24	641.0
25	1059.0
26	1651.0
27	2295.0
28	3609.0
29	5052.0
30	7208.0
31	9923.0
32	13523.0
33	18473.0
34	26886.0
35	40369.0
36	64156.0
37	117720.0
38	281622.0
39	491651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12876407285911	17.364907453841944	12.157754047600426	27.348574425698523
2	19.519118844633795	19.41569166923548	32.9878399455259	28.07734954060483
3	20.226914069868553	20.70337840635654	28.86225506208851	30.207452461686398
4	15.629097634701475	15.060800272370498	32.4029795124016	36.90712258052643
5	16.869119534761744	33.90340969215693	32.677006316971166	16.550464456110163
6	36.80884836048604	32.62759315577108	15.067057432447974	15.496501051294912
7	31.29168947922945	28.60101862885379	20.039935404023907	20.067356487892855
8	28.776587179263036	31.64751945010605	19.09059539638649	20.485297974244425
9	27.47776637788646	13.825195191188447	18.35878371850141	40.338254712423684
10	18.078131685614512	25.27257753587515	31.625527372774915	25.023763405735423
11	38.17024076263739	20.193235825922127	20.227374155168366	21.409149256272116
12	23.616546507722532	23.663291174183694	27.62453358852731	25.09562872956646
13	32.25667238706056	18.070310235517663	22.54427170797466	27.128745669447113
14	24.16441608274174	18.858620388219975	23.188575161835004	33.78838836720328
15	26.919590892151408	25.539887095067428	20.63335342372476	26.90716858905641
16	26.844781022401556	24.17131736223896	22.502679996871418	26.481221618488064
17	25.658773136539516	24.332439234234027	23.77361962907923	26.23516800014723
18	25.952583609001106	23.29402671255251	24.518497729479044	26.234891948967338
19	26.59495470460223	23.701754305248194	24.039548932372064	25.66374205777751
20	26.853430626038065	23.486986487294743	23.753835961187203	25.905746925479985
21	27.318116778850797	23.32089569406168	23.404355167448045	25.956632359639475
22	27.472613422528536	23.367548343462875	23.009878031387018	26.149960202621564
23	26.87845926634798	23.30736918624713	23.408679969266302	26.405491578138584
24	26.61096567303578	23.4471431003308	23.246729943731566	26.695161282901854
25	26.650440991759872	22.888599546355895	23.685375268574795	26.775584193309438
26	26.37623015307038	23.33442220187623	23.809690316584696	26.479657328468697
27	26.297095481502268	23.223633661680875	23.706907260606116	26.772363596210734
28	26.332522049587993	23.304332623268355	23.729359423237067	26.63378590390658
29	25.987734125906943	23.699821946988973	24.041021205331468	26.27142272177262
30	25.857990071359232	23.777852413837525	23.887996834613137	26.47616068019011
31	26.46429047945489	23.513671434683992	23.49940879038974	26.52262929547138
32	25.587459915068255	23.577531274298256	24.05749225906483	26.77751655156866
33	25.51154584059885	23.148823791931026	24.558157082323063	26.78147328514707
34	26.218328878174013	22.44746976089367	24.571775607197573	26.762425753734743
35	25.6312600356106	22.74532898399363	25.152495272623543	26.470915707772217
36	25.443637250346214	22.412043192807946	25.181848714751716	26.96247084209412
37	25.10970733974079	21.63707551380026	25.85237703070149	27.400840115757465
38	24.430069334854682	21.53972146435949	26.801901072458833	27.228308128326994
39	24.271615957598538	21.006942687174202	27.70698087425409	27.014460480973174
40	23.97136429093954	21.008967062493387	28.26101559229081	26.75865305427626
41	23.11716992330378	21.030775105704596	28.920133792805185	26.931921178186435
42	22.679812837300034	21.305722080873792	29.146403743254	26.86806133857217
43	21.99014497287797	20.91382142249173	29.543273322874057	27.55276028175624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	32.0
2	44.0
3	118.0
4	192.0
5	192.0
6	245.0
7	298.0
8	315.0
9	332.0
10	470.0
11	608.0
12	608.0
13	959.5
14	1311.0
15	2009.5
16	2708.0
17	2750.5
18	2793.0
19	2793.0
20	3309.0
21	3825.0
22	4005.0
23	4185.0
24	5236.0
25	6287.0
26	6287.0
27	7613.5
28	8940.0
29	11101.5
30	13263.0
31	15655.5
32	18048.0
33	18048.0
34	21517.5
35	24987.0
36	29216.0
37	33445.0
38	38137.0
39	42829.0
40	42829.0
41	48203.0
42	53577.0
43	58810.5
44	64044.0
45	67596.5
46	71149.0
47	71149.0
48	74838.5
49	78528.0
50	81706.5
51	84885.0
52	86714.5
53	88544.0
54	88544.0
55	86836.0
56	85128.0
57	82819.5
58	80511.0
59	77879.5
60	75248.0
61	75248.0
62	70025.0
63	64802.0
64	58353.0
65	51904.0
66	46047.5
67	40191.0
68	40191.0
69	34675.5
70	29160.0
71	24878.5
72	20597.0
73	16787.0
74	12977.0
75	12977.0
76	10571.0
77	8165.0
78	6818.5
79	5472.0
80	4552.5
81	3633.0
82	3633.0
83	2821.0
84	2009.0
85	1574.5
86	1140.0
87	875.0
88	610.0
89	610.0
90	450.0
91	290.0
92	169.0
93	48.0
94	32.5
95	17.0
96	17.0
97	12.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1086755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.36522489502718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13615924384719	62.898568258210844
2	7.023271762473287	10.024347376556001
3	1.8868131782408208	4.039585404001715
4	0.8167707091244791	2.331561013773571
5	0.4880497353084958	1.7414889560124645
6	0.31107605454790704	1.3320007555361475
7	0.22081859464429537	1.1031138067455788
8	0.14681531804829068	0.8382006552440966
9	0.12071929303675805	0.7753643547063234
>10	0.7815583635312363	10.42004339101739
>50	0.05116814099194038	2.4842371298355297
>100	0.016392385471084268	1.7065927192492831
>500	1.2907357808197598E-4	0.0864026030230085
>1k	2.5814715616395196E-4	0.21849357608803432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.11860999029220018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.201705996291713E-5	0.0
3	0.0	0.0	0.0	9.201705996291713E-5	0.0
4	0.0	0.0	0.0	9.201705996291713E-5	0.0
5	0.0	9.201705996291713E-5	0.0	9.201705996291713E-5	0.0
6	0.0	9.201705996291713E-5	0.0	9.201705996291713E-5	0.0
7	0.0	9.201705996291713E-5	0.0	9.201705996291713E-5	0.0
8	0.0	9.201705996291713E-5	0.0	9.201705996291713E-5	0.0
9	0.0	9.201705996291713E-5	0.0	2.760511798887514E-4	0.0
10	0.0	9.201705996291713E-5	0.0	3.680682398516685E-4	0.0
11	0.0	9.201705996291713E-5	0.0	3.680682398516685E-4	0.0
12	0.0	9.201705996291713E-5	0.0	3.680682398516685E-4	0.0
13	0.0	9.201705996291713E-5	0.0	4.6008529981458564E-4	0.0
14	0.0	9.201705996291713E-5	0.0	6.441194197404199E-4	0.0
15	0.0	9.201705996291713E-5	0.0	6.441194197404199E-4	0.0
16	0.0	9.201705996291713E-5	0.0	7.36136479703337E-4	0.0
17	0.0	9.201705996291713E-5	0.0	0.0010121876595920883	0.0
18	0.0	9.201705996291713E-5	0.0	0.0011042047195550055	0.0
19	0.0	9.201705996291713E-5	0.0	0.001472272959406674	0.0
20	0.0	9.201705996291713E-5	0.0	0.001472272959406674	0.0
21	0.0	9.201705996291713E-5	0.0	0.0017483241392954253	0.0
22	0.0	9.201705996291713E-5	0.0	0.002852528858850431	0.0
23	0.0	9.201705996291713E-5	0.0	0.0041407676983312705	0.0
24	0.0	9.201705996291713E-5	0.0	0.006993296557181701	0.0
25	0.0	9.201705996291713E-5	0.0	0.008465569516588375	0.0
26	0.0	9.201705996291713E-5	0.0	0.01279037133484548	0.0
27	0.0	9.201705996291713E-5	0.0	0.04821693942056857	0.0
28	0.0	9.201705996291713E-5	0.0	0.16544667381332498	0.0
29	0.0	9.201705996291713E-5	0.0	0.33162948410635334	0.0
30	0.0	9.201705996291713E-5	0.0	0.5532065644970577	0.0
31	0.0	9.201705996291713E-5	0.0	1.200270530156291	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	580	0.0	27.11207	1
GTACTAT	110	1.7553248E-9	20.181818	1
CTTATAC	1545	0.0	18.55987	37
GTTAACG	70	1.2191195E-4	18.5	13
GTATCAA	885	0.0	17.559322	2
TTGTATG	170	8.54925E-11	16.32353	4
TTGCGCG	105	9.342082E-6	15.857142	18
GACTGCG	105	9.342082E-6	15.857142	7
GACGTAG	85	5.363815E-4	15.235294	1
TAGTCTT	85	5.363815E-4	15.235294	5
TATGCCG	75	0.004104214	14.8	33
TCGTATG	75	0.004104214	14.8	30
TACTATA	75	0.004104214	14.8	2
TCTTATA	2335	0.0	14.182013	37
ATACACA	420	0.0	14.095238	37
ATTTGCG	120	3.300033E-5	13.875001	16
GGACGGT	95	0.0012449974	13.631579	6
TTAACTC	110	2.4561665E-4	13.454545	3
TACTATT	110	2.4561665E-4	13.454545	2
GTTTAGA	125	4.8439062E-5	13.32	1
>>END_MODULE