FastQCFastQC Report
Fri 10 Feb 2017
ERR1632049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144065
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.38732516572380526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4940.34290077395620033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3420.23739284350813866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.13396730642418353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT250.005480595429.59999814
ACTGTGC250.005480595429.5999988
TGTACTT250.005480595429.5999985
CGAAGTG250.005480595429.5999984
GCGAAAG400.001923042323.12500218
CGCAAGA400.001923042323.1250022
CGGGTTA450.003809828320.5555559
CGGCGTA450.003809828320.55555527
AGAGCGA450.003809828320.55555515
TGCCAAG656.848721E-519.92307729
CGCTGTC656.848721E-519.92307734
ACCAGAG609.18346E-418.512
TTGCCAA609.18346E-418.528
TTCCGGA500.007005779518.4999982
ACGGGTT500.007005779518.4999988
GACCAGA500.007005779518.49999811
AACGGGT500.007005779518.4999987
CATTTGC500.007005779518.49999825
GCGTAGG500.007005779518.49999829
ATACAGG500.007005779518.4999986
Produced by FastQC (version 0.11.5)