##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144065
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58331308784229	34.0	31.0	34.0	31.0	34.0
2	32.76269045222642	34.0	31.0	34.0	31.0	34.0
3	32.83913511262278	34.0	31.0	34.0	31.0	34.0
4	36.289001492381914	37.0	37.0	37.0	35.0	37.0
5	36.131877971748864	37.0	35.0	37.0	35.0	37.0
6	36.16688300419949	37.0	36.0	37.0	35.0	37.0
7	36.13744490334224	37.0	35.0	37.0	35.0	37.0
8	36.13870128067192	37.0	35.0	37.0	35.0	37.0
9	37.881539582827195	39.0	38.0	39.0	35.0	39.0
10	37.83481761704786	39.0	38.0	39.0	35.0	39.0
11	37.91188699545344	39.0	38.0	39.0	35.0	39.0
12	37.86927428591261	39.0	38.0	39.0	35.0	39.0
13	37.89771283795509	39.0	38.0	39.0	35.0	39.0
14	39.24014160274876	40.0	39.0	41.0	36.0	41.0
15	39.25011626696283	40.0	39.0	41.0	36.0	41.0
16	39.2300697601777	40.0	39.0	41.0	36.0	41.0
17	39.234074896748	40.0	39.0	41.0	36.0	41.0
18	39.24108562107382	40.0	39.0	41.0	36.0	41.0
19	39.25752958733905	40.0	39.0	41.0	36.0	41.0
20	39.23964182834138	40.0	39.0	41.0	36.0	41.0
21	39.22315621420886	40.0	39.0	41.0	36.0	41.0
22	39.16282233713949	40.0	39.0	41.0	36.0	41.0
23	39.12707458438899	40.0	39.0	41.0	36.0	41.0
24	39.07087772880297	40.0	39.0	41.0	36.0	41.0
25	39.02430153055912	40.0	39.0	41.0	35.0	41.0
26	38.916933328705795	40.0	38.0	41.0	35.0	41.0
27	38.8388435775518	40.0	38.0	41.0	35.0	41.0
28	38.77934959913928	40.0	38.0	41.0	35.0	41.0
29	38.69206955193836	40.0	38.0	41.0	35.0	41.0
30	38.61443098601326	40.0	38.0	41.0	35.0	41.0
31	38.50044077326207	40.0	38.0	41.0	35.0	41.0
32	38.418665185853605	40.0	38.0	41.0	34.0	41.0
33	38.32195189671329	40.0	38.0	41.0	34.0	41.0
34	38.2451324055114	40.0	38.0	41.0	34.0	41.0
35	38.2089751153993	40.0	38.0	41.0	34.0	41.0
36	38.15353486273557	40.0	38.0	41.0	34.0	41.0
37	38.06984347343213	40.0	37.0	41.0	34.0	41.0
38	38.00864193246104	40.0	37.0	41.0	33.0	41.0
39	37.934814146392256	40.0	37.0	41.0	33.0	41.0
40	37.82319786207615	40.0	37.0	41.0	33.0	41.0
41	37.75457605941762	40.0	37.0	41.0	33.0	41.0
42	37.67795092492972	40.0	37.0	41.0	33.0	41.0
43	36.80651789122965	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	6.0
20	10.0
21	21.0
22	34.0
23	64.0
24	119.0
25	170.0
26	304.0
27	422.0
28	664.0
29	909.0
30	1200.0
31	1627.0
32	2287.0
33	2893.0
34	4114.0
35	6074.0
36	9391.0
37	17622.0
38	39716.0
39	56415.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.07059313504321	18.83385971610037	12.81851941831812	26.2770277305383
2	19.695970568840455	20.234616319022663	33.805573872904596	26.26383923923229
3	20.589317322042135	21.926213861798495	29.478360462291324	28.006108353868047
4	15.567278658938674	15.703328358726964	33.571651685003296	35.157741297331064
5	16.22045604414674	34.70100301947038	32.56654982126123	16.511991115121646
6	35.10637559434977	33.55291014472634	15.35695692916392	15.983757331759971
7	29.732412452712314	29.32912227119703	20.876687606288826	20.061777669801824
8	27.817998819977092	31.98972685940374	19.607815916426613	20.584458404192553
9	26.921181411168572	14.039496060805886	20.10134314371985	38.93797938430569
10	18.48610002429459	25.744629160448408	31.47676396071218	24.292506854544822
11	36.48769652587374	21.216117724638185	20.512268767570195	21.783916981917884
12	22.553014264394545	25.154617707284903	27.89227084996356	24.40009717835699
13	31.190781938708223	18.910214139450943	23.814250511921703	26.08475340991913
14	23.780932218095998	20.765626626869814	24.29458924790893	31.158851907125257
15	26.734460139520355	26.438066150695867	21.548606531773853	25.27886717800993
16	25.675216048311526	25.269149342310765	23.600458126540104	25.45517648283761
17	24.15923367924201	25.75573525839031	24.74577447679867	25.339256585569014
18	24.606254121403534	24.168257383819803	25.824454239405824	25.401034255370842
19	24.884600701072433	24.85822371846042	26.01811682226773	24.239058758199423
20	25.171970985319124	24.50699337104779	25.623156214208866	24.69787942942422
21	26.174296324575714	24.186998924096763	25.197653836809774	24.441050914517753
22	26.012563773296776	24.11133863186756	24.929719223961406	24.94637837087426
23	25.497518481241105	24.203658071009613	25.1747475098046	25.124075937944678
24	24.936660535175097	24.75479818137646	24.984555582549543	25.3239857008989
25	25.24554888418422	24.268906396418284	25.138652691493423	25.346892027904072
26	24.926942699475934	24.836011522576616	25.227501475028635	25.009544302918822
27	25.030368236559887	24.425780029847637	25.14559400270711	25.398257730885366
28	24.319577968278207	24.5847360566411	26.032693575816474	25.06299239926422
29	24.29389511678756	24.931801617325515	26.144448686356853	24.629854579530072
30	24.14673931905737	25.31704438968521	25.70853434213723	24.82768194912019
31	24.937354666296464	24.85683545621768	25.230972130635475	24.97483774685038
32	23.97598306320064	24.56113559851456	25.941762398917156	25.52111893936765
33	23.775379169125046	24.30569534585083	26.282580779509253	25.636344705514873
34	24.479922257314406	24.068996633464064	25.975774823864228	25.475306285357302
35	24.334154721826952	24.036372470759726	26.490125984798528	25.139346822614794
36	24.036372470759726	24.374414326866344	26.09308298337556	25.49613021899837
37	24.055114011036686	23.381112692187557	27.060007635442336	25.50376566133343
38	23.5782459306563	23.013917328983442	27.974872453406448	25.43296428695381
39	23.22979210772915	22.444035678339638	28.880713566792764	25.445458647138448
40	22.789712976781313	22.8389962863985	29.27081525700205	25.100475479818137
41	22.321174469857354	22.887585464894318	29.49849026481102	25.292749800437303
42	22.012980251969598	23.030576475896297	29.55263249227779	25.403810779856318
43	21.302884114809288	22.773053829868463	29.67618783188144	26.24787422344081
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	12.0
2	17.0
3	40.5
4	64.0
5	64.0
6	97.5
7	131.0
8	117.5
9	104.0
10	166.0
11	228.0
12	228.0
13	398.5
14	569.0
15	863.0
16	1157.0
17	1043.5
18	930.0
19	930.0
20	1078.5
21	1227.0
22	1095.0
23	963.0
24	1116.0
25	1269.0
26	1269.0
27	1475.5
28	1682.0
29	1956.0
30	2230.0
31	2710.0
32	3190.0
33	3190.0
34	3770.5
35	4351.0
36	5034.5
37	5718.0
38	6295.5
39	6873.0
40	6873.0
41	7553.5
42	8234.0
43	8557.0
44	8880.0
45	9421.5
46	9963.0
47	9963.0
48	10188.0
49	10413.0
50	10476.0
51	10539.0
52	10566.5
53	10594.0
54	10594.0
55	10392.5
56	10191.0
57	9810.5
58	9430.0
59	8980.5
60	8531.0
61	8531.0
62	7831.0
63	7131.0
64	6401.0
65	5671.0
66	5056.5
67	4442.0
68	4442.0
69	3837.5
70	3233.0
71	2747.5
72	2262.0
73	1898.5
74	1535.0
75	1535.0
76	1237.5
77	940.0
78	734.5
79	529.0
80	452.0
81	375.0
82	375.0
83	295.5
84	216.0
85	176.5
86	137.0
87	103.5
88	70.0
89	70.0
90	52.0
91	34.0
92	18.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	144065.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.53630376772729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.81609314438177	71.32360278185384
2	5.220119364949074	8.199377602988724
3	1.486555085608188	3.502456253123535
4	0.7574416370586207	2.379466659774419
5	0.43047444904564736	1.6903936047247003
6	0.2886260441442564	1.3600573606914472
7	0.20772845712381927	1.1419957646914307
8	0.14680783029934716	0.9223795484696389
9	0.11085486168636559	0.7835517982376821
>10	0.5203187397440889	6.88333357423605
>50	0.0109856163698048	0.5686436687396935
>100	0.0029960771917649453	0.8070818415629579
>500	9.986923972549818E-4	0.4376595409058606
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.38732516572380526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.34290077395620033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.23739284350813866	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	193	0.13396730642418353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	6.941311213688266E-4	0.0
16	0.0	0.0	0.0	6.941311213688266E-4	0.0
17	0.0	0.0	0.0	0.0013882622427376532	0.0
18	0.0	0.0	0.0	0.0013882622427376532	0.0
19	0.0	0.0	0.0	0.0013882622427376532	0.0
20	0.0	0.0	0.0	0.0013882622427376532	0.0
21	0.0	0.0	0.0	0.00208239336410648	0.0
22	0.0	0.0	0.0	0.00208239336410648	0.0
23	0.0	0.0	0.0	0.0027765244854753065	0.0
24	0.0	0.0	0.0	0.0034706556068441327	0.0
25	0.0	0.0	0.0	0.005553048970950613	0.0
26	0.0	0.0	0.0	0.007635442335057092	0.0
27	0.0	0.0	0.0	0.03678894943254781	0.0
28	0.0	0.0	0.0	0.1256377329677576	0.0
29	0.0	0.0	0.0	0.22559261444486864	0.0
30	0.0	0.0	0.0	0.3741366744177975	0.0
31	0.0	0.0	0.0	0.8072744941519453	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCAAT	25	0.0054805954	29.599998	14
ACTGTGC	25	0.0054805954	29.599998	8
TGTACTT	25	0.0054805954	29.599998	5
CGAAGTG	25	0.0054805954	29.599998	4
GCGAAAG	40	0.0019230423	23.125002	18
CGCAAGA	40	0.0019230423	23.125002	2
CGGGTTA	45	0.0038098283	20.555555	9
CGGCGTA	45	0.0038098283	20.555555	27
AGAGCGA	45	0.0038098283	20.555555	15
TGCCAAG	65	6.848721E-5	19.923077	29
CGCTGTC	65	6.848721E-5	19.923077	34
ACCAGAG	60	9.18346E-4	18.5	12
TTGCCAA	60	9.18346E-4	18.5	28
TTCCGGA	50	0.0070057795	18.499998	2
ACGGGTT	50	0.0070057795	18.499998	8
GACCAGA	50	0.0070057795	18.499998	11
AACGGGT	50	0.0070057795	18.499998	7
CATTTGC	50	0.0070057795	18.499998	25
GCGTAGG	50	0.0070057795	18.499998	29
ATACAGG	50	0.0070057795	18.499998	6
>>END_MODULE