FastQCFastQC Report
Fri 10 Feb 2017
ERR1632048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194840
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG2270.11650585095462944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT250.00548528329.5999984
GGTATCA1050.028.1904751
CCGACCA358.8428677E-426.428579
CCATAAA400.001925465723.12500213
CATAAAC400.001925465723.12500214
GTATCAA1500.019.7333342
CTTATAC1409.276846E-1118.537
TGACCCG500.007014439418.49999813
CGCCGGG500.007014439418.49999818
CCTATGA700.00258214515.8571421
TCTTATA1701.4570105E-915.23529436
CGAACCT750.00408815814.826
CTCTTAT2800.014.53571437
GCCCAGC800.00627389213.8750011
GATGCCG800.00627389213.87500121
TATCAAC2501.0913936E-1113.323
ATCAACG2253.965397E-1013.1555564
TCAACGC2253.965397E-1013.1555565
TCCTGGT850.00936811313.0588242
CCTCGGG850.00936811313.05882411
Produced by FastQC (version 0.11.5)