##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194840
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45894066926709	34.0	31.0	34.0	31.0	34.0
2	32.64232190515295	34.0	31.0	34.0	31.0	34.0
3	32.73182611373434	34.0	31.0	34.0	31.0	34.0
4	36.22067337302402	37.0	37.0	37.0	35.0	37.0
5	36.028854444672554	37.0	35.0	37.0	35.0	37.0
6	36.046427838226236	37.0	35.0	37.0	35.0	37.0
7	36.016552042701704	37.0	35.0	37.0	35.0	37.0
8	36.0054762882365	37.0	35.0	37.0	35.0	37.0
9	37.7562410182714	39.0	37.0	39.0	35.0	39.0
10	37.67166392937795	39.0	37.0	39.0	35.0	39.0
11	37.750903305276125	39.0	37.0	39.0	35.0	39.0
12	37.70740094436461	39.0	37.0	39.0	35.0	39.0
13	37.732267501539724	39.0	37.0	39.0	35.0	39.0
14	39.06128105111887	40.0	38.0	41.0	36.0	41.0
15	39.05537363990967	40.0	38.0	41.0	36.0	41.0
16	39.04478033258058	40.0	38.0	41.0	36.0	41.0
17	39.00823752822829	40.0	38.0	41.0	36.0	41.0
18	39.0038852391706	40.0	38.0	41.0	36.0	41.0
19	39.008247793060974	40.0	38.0	41.0	36.0	41.0
20	38.990166290289466	40.0	38.0	41.0	36.0	41.0
21	38.96389858345309	40.0	38.0	41.0	36.0	41.0
22	38.93505953602956	40.0	38.0	41.0	36.0	41.0
23	38.879788544446725	40.0	38.0	41.0	35.0	41.0
24	38.8161568466434	40.0	38.0	41.0	35.0	41.0
25	38.755994662287	40.0	38.0	41.0	35.0	41.0
26	38.65307944980497	40.0	38.0	41.0	35.0	41.0
27	38.56536132211045	40.0	38.0	41.0	35.0	41.0
28	38.49455963867789	40.0	38.0	41.0	34.0	41.0
29	38.41672141244098	40.0	38.0	41.0	34.0	41.0
30	38.34402073496202	40.0	38.0	41.0	34.0	41.0
31	38.235234038185176	40.0	38.0	41.0	34.0	41.0
32	38.15884828577294	40.0	38.0	41.0	34.0	41.0
33	38.07061178402792	40.0	38.0	41.0	33.0	41.0
34	37.968425374666396	40.0	37.0	41.0	33.0	41.0
35	37.957241839458014	40.0	37.0	41.0	33.0	41.0
36	37.90420344898378	40.0	37.0	41.0	33.0	41.0
37	37.82265961814822	40.0	37.0	41.0	33.0	41.0
38	37.775076986245125	40.0	37.0	41.0	33.0	41.0
39	37.70044652022172	40.0	37.0	41.0	33.0	41.0
40	37.6248768220078	40.0	37.0	41.0	33.0	41.0
41	37.54162902894683	39.0	36.0	41.0	33.0	41.0
42	37.48118969410799	39.0	36.0	41.0	33.0	41.0
43	36.55200677478957	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	7.0
19	5.0
20	16.0
21	40.0
22	71.0
23	96.0
24	196.0
25	268.0
26	425.0
27	659.0
28	936.0
29	1267.0
30	1816.0
31	2506.0
32	3378.0
33	4494.0
34	6452.0
35	9312.0
36	15283.0
37	26897.0
38	58084.0
39	62629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30404434407719	17.48203654280435	11.568466433997125	26.645452679121327
2	21.104495996715254	19.70950523506467	31.425785259700266	27.76021350851981
3	21.779408745637447	20.30537877232601	27.964483678916036	29.95072880312051
4	15.734962020119072	15.284849106959555	31.895401354957915	37.084787517963456
5	17.60213508519811	33.1323136932868	32.049887086840485	17.215664134674604
6	37.68887292137138	31.75169369739273	14.858345308971463	15.70108807226442
7	31.81944159310203	27.580065694929175	19.599671525354136	21.000821186614658
8	29.06692670909464	30.006158899609936	19.20550195031821	21.72141244097721
9	27.50461917470745	12.873639909669473	18.327345514268117	41.294395401354954
10	18.493122562102236	24.31482241839458	31.664442619585305	25.52761239991788
11	38.396633134879906	19.828064052555945	19.34561691644426	22.429685896119896
12	23.47515910490659	23.02658591664956	26.833299117224392	26.664955861219465
13	33.10408540340792	17.6739889139807	22.015499897351674	27.206425785259704
14	25.001539724902482	19.055635393143092	22.205912543625537	33.736912338328885
15	27.129439540135497	25.56405255594334	20.182200780127285	27.124307123793884
16	28.344795729829602	23.118969410798602	21.716280024635598	26.819954834736194
17	26.461712174091563	23.85906384725929	22.755081092178198	26.924142886470953
18	26.362143297064257	22.25467049887087	23.992506672141246	27.390679531923627
19	27.6129131595155	23.3063026072675	23.267296243071236	25.813487990145763
20	27.49281461712174	22.824881954424143	23.1323136932868	26.54998973516732
21	27.47587764319442	23.1256415520427	22.648840073906797	26.74964073085609
22	28.381235885855062	22.46253336070622	22.366557175118047	26.789673578320674
23	26.304146992404025	22.779716690617942	23.004003284746457	27.912133032231573
24	26.936973927324985	23.246766577704783	22.54875795524533	27.267501539724904
25	27.202833093820573	22.628310408540344	23.002976801478138	27.165879696160957
26	27.64678710737015	23.107164853212893	22.225928967357834	27.020119072059124
27	27.403510572777662	22.848491069595568	22.28135906384726	27.46663929377951
28	26.751180455758572	23.11794292753028	22.99630466023404	27.13457195647711
29	26.862040648737423	23.176965715458838	23.533155409566824	26.42783822623691
30	26.688564976390882	23.08355573804147	22.809997947033462	27.417881338534183
31	26.57770478341203	22.975774994867585	22.836173270375692	27.610346951344695
32	26.030589201396015	23.29552453295011	23.017860808868814	27.656025456785056
33	26.16043933483884	22.486655717511805	23.572161773763085	27.780743173886265
34	27.132005748306305	22.603674810100596	23.531615684664338	26.73270375692876
35	26.936973927324985	22.238760008211866	23.4936358037364	27.33063026072675
36	25.97207965510162	22.74789570929994	23.259597618558818	28.020427017039623
37	26.01570519400534	21.876924656128104	24.93943748716896	27.1679326626976
38	25.563539314309175	21.567953192362964	24.91018271402176	27.9583247793061
39	25.26688564976391	21.684972284951755	26.23896530486553	26.809176760418808
40	25.68055840689797	21.00646684459043	26.148634777253132	27.164339971258467
41	24.37897762266475	21.41192773557791	27.001642373229316	27.20745226852802
42	24.230650790392115	21.919523711763496	26.833299117224392	27.01652638062
43	23.894990761650586	20.88893451036748	26.942106343666598	28.273968384315335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	19.5
4	35.0
5	35.0
6	29.0
7	23.0
8	26.0
9	29.0
10	43.0
11	57.0
12	57.0
13	93.5
14	130.0
15	193.0
16	256.0
17	277.5
18	299.0
19	299.0
20	394.5
21	490.0
22	560.0
23	630.0
24	777.0
25	924.0
26	924.0
27	1120.0
28	1316.0
29	1642.0
30	1968.0
31	2376.5
32	2785.0
33	2785.0
34	3645.5
35	4506.0
36	5140.0
37	5774.0
38	6274.5
39	6775.0
40	6775.0
41	7505.5
42	8236.0
43	9101.0
44	9966.0
45	10510.0
46	11054.0
47	11054.0
48	11612.0
49	12170.0
50	13122.0
51	14074.0
52	15256.5
53	16439.0
54	16439.0
55	16360.0
56	16281.0
57	15756.0
58	15231.0
59	15223.0
60	15215.0
61	15215.0
62	14455.0
63	13695.0
64	11726.5
65	9758.0
66	8968.0
67	8178.0
68	8178.0
69	7179.5
70	6181.0
71	5914.5
72	5648.0
73	4589.5
74	3531.0
75	3531.0
76	2415.0
77	1299.0
78	1028.0
79	757.0
80	639.5
81	522.0
82	522.0
83	407.0
84	292.0
85	225.0
86	158.0
87	125.0
88	92.0
89	92.0
90	68.0
91	44.0
92	26.5
93	9.0
94	7.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	194840.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.61706235792294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63223659568182	55.11288397713824
2	7.200276257165353	9.161208472927205
3	2.165477708991338	4.132839913427822
4	1.0476393852134556	2.665909603909618
5	0.6678560202893213	2.124351904443
6	0.4173823724691458	1.593158424988048
7	0.29600743149391495	1.3181786259429893
8	0.20658145752261045	1.0513684372165215
9	0.15592311301448888	0.8927433363315785
>10	1.0437747417509136	12.820423128902657
>50	0.12314743830090501	5.302037352532833
>100	0.04369747810677274	3.824896822239486
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	227	0.11650585095462944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.132416341613632E-4	0.0
18	0.0	0.0	0.0	0.0010264832683227264	0.0
19	0.0	0.0	0.0	0.0010264832683227264	0.0
20	0.0	0.0	0.0	0.0010264832683227264	0.0
21	0.0	0.0	0.0	0.0010264832683227264	0.0
22	0.0	0.0	0.0	0.0015397249024840895	0.0
23	0.0	0.0	0.0	0.0041059330732909054	0.0
24	0.0	0.0	0.0	0.006158899609936358	0.0
25	0.0	0.0	0.0	0.008211866146581811	0.0
26	0.0	0.0	0.0	0.01591049065900226	0.0
27	0.0	0.0	0.0	0.04413878053787723	0.0
28	0.0	0.0	0.0	0.10572777663724081	0.0
29	0.0	0.0	0.0	0.1827140217614453	0.0
30	0.0	0.0	0.0	0.27817696571545886	0.0
31	0.0	0.0	0.0	0.53839047423527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGAT	25	0.005485283	29.599998	4
GGTATCA	105	0.0	28.190475	1
CCGACCA	35	8.8428677E-4	26.42857	9
CCATAAA	40	0.0019254657	23.125002	13
CATAAAC	40	0.0019254657	23.125002	14
GTATCAA	150	0.0	19.733334	2
CTTATAC	140	9.276846E-11	18.5	37
TGACCCG	50	0.0070144394	18.499998	13
CGCCGGG	50	0.0070144394	18.499998	18
CCTATGA	70	0.002582145	15.857142	1
TCTTATA	170	1.4570105E-9	15.235294	36
CGAACCT	75	0.004088158	14.8	26
CTCTTAT	280	0.0	14.535714	37
GCCCAGC	80	0.006273892	13.875001	1
GATGCCG	80	0.006273892	13.875001	21
TATCAAC	250	1.0913936E-11	13.32	3
ATCAACG	225	3.965397E-10	13.155556	4
TCAACGC	225	3.965397E-10	13.155556	5
TCCTGGT	85	0.009368113	13.058824	2
CCTCGGG	85	0.009368113	13.058824	11
>>END_MODULE