FastQCFastQC Report
Fri 10 Feb 2017
ERR1632047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183691
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.13718690627194582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2490.13555372881632743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1920.10452335715957776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG250.005484474429.61
TCTAATA250.005484474429.62
TCGGACT400.001925048523.1254
TATACAC502.690141E-422.236
GGTATCA1350.021.9259241
TCAGGAC705.0635117E-621.1428573
CGCGGTT450.003813761520.55555723
TAAGGCT450.003813761520.5555574
ACAGTGC701.2121339E-418.58
GTTTATG500.00701294518.51
GTGTGGT500.00701294518.51
CGCGTGC609.196595E-418.59
TGCAGGG609.196595E-418.52
CTCTAAT500.00701294518.51
CTCAGTT500.00701294518.529
CTTATAC1552.0008883E-1117.90322737
CGCGCGT650.001573110217.0769237
GTATCAA1757.2759576E-1216.9142862
TAGCGCG700.002581389215.8571424
AACTGTC957.008187E-515.57894836
Produced by FastQC (version 0.11.5)