FastQCFastQC Report
Fri 10 Feb 2017
ERR1632046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258117
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6370.2467873096309038No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT2950.11428925642247509No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG2870.11118988675678085No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG2610.10111693534327457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT200.001839292737.01
AGAGCGA303.591816E-430.83333410
GGTATCA1100.025.2272721
ATTCCGA450.00381789520.55555528
AATAACG450.00381789520.5555552
TAATTTT555.128257E-420.18181814
TAGAAGT656.875664E-519.9230754
TATTAAA609.2103845E-418.52
TAGAACT500.007020472518.4999984
CTCGCGG500.007020472518.49999822
TGCATCA2150.018.06976714
TCCAGGA3000.016.652
CTGCATC2350.016.53191413
CAGGACA2550.015.9607844
TGAACAC700.002585197815.8571425
GCTGCAT2450.015.85714212
ATCAAGC2350.015.74467930
AAGAGGC2400.015.41666722
CCAGGAC3000.015.4166673
GCATCAG2400.015.41666715
Produced by FastQC (version 0.11.5)