##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258117
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75497158265438	34.0	31.0	34.0	31.0	34.0
2	32.93373935075954	34.0	31.0	34.0	31.0	34.0
3	33.01073544167955	34.0	33.0	34.0	31.0	34.0
4	36.402801830177786	37.0	37.0	37.0	35.0	37.0
5	36.27275227900526	37.0	37.0	37.0	35.0	37.0
6	36.290635641976316	37.0	37.0	37.0	35.0	37.0
7	36.27427484435353	37.0	37.0	37.0	35.0	37.0
8	36.259855026983885	37.0	37.0	37.0	35.0	37.0
9	38.062828097335704	39.0	38.0	39.0	35.0	39.0
10	38.019669374740914	39.0	38.0	39.0	35.0	39.0
11	38.09246581976391	39.0	38.0	39.0	37.0	39.0
12	38.051437913814276	39.0	38.0	39.0	35.0	39.0
13	38.070584269924105	39.0	38.0	39.0	35.0	39.0
14	39.473827760279256	40.0	39.0	41.0	37.0	41.0
15	39.493582367685974	40.0	39.0	41.0	37.0	41.0
16	39.485818446673406	40.0	39.0	41.0	37.0	41.0
17	39.45792411968216	40.0	39.0	41.0	37.0	41.0
18	39.42925107606241	40.0	39.0	41.0	37.0	41.0
19	39.44194299484342	40.0	39.0	41.0	37.0	41.0
20	39.41388207673265	40.0	39.0	41.0	37.0	41.0
21	39.39962110205837	40.0	39.0	41.0	37.0	41.0
22	39.34473513949101	40.0	39.0	41.0	36.0	41.0
23	39.30159578795662	40.0	39.0	41.0	36.0	41.0
24	39.25331148277719	40.0	39.0	41.0	36.0	41.0
25	39.18627986533239	40.0	39.0	41.0	36.0	41.0
26	39.09431769313916	40.0	39.0	41.0	36.0	41.0
27	39.02690252869822	40.0	39.0	41.0	35.0	41.0
28	38.95091760713165	40.0	39.0	41.0	35.0	41.0
29	38.89042178546938	40.0	38.0	41.0	35.0	41.0
30	38.80900134435159	40.0	38.0	41.0	35.0	41.0
31	38.75715276405661	40.0	38.0	41.0	35.0	41.0
32	38.67496135473448	40.0	38.0	41.0	35.0	41.0
33	38.58786906712847	40.0	38.0	41.0	35.0	41.0
34	38.50967971888717	40.0	38.0	41.0	34.0	41.0
35	38.47500552075222	40.0	38.0	41.0	34.0	41.0
36	38.45516568067969	40.0	38.0	41.0	34.0	41.0
37	38.38126508521329	40.0	38.0	41.0	34.0	41.0
38	38.33270571097603	40.0	38.0	41.0	34.0	41.0
39	38.280399973655356	40.0	38.0	41.0	34.0	41.0
40	38.185028494829865	40.0	38.0	41.0	34.0	41.0
41	38.14301653901138	40.0	37.0	41.0	34.0	41.0
42	38.08181173653808	40.0	37.0	41.0	34.0	41.0
43	37.16848560923922	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	6.0
20	11.0
21	27.0
22	59.0
23	96.0
24	202.0
25	279.0
26	425.0
27	595.0
28	807.0
29	1240.0
30	1757.0
31	2353.0
32	3181.0
33	4408.0
34	6521.0
35	10299.0
36	15457.0
37	27442.0
38	66012.0
39	116937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9680454987467	16.377456734736572	11.526168365508664	28.128329401008067
2	21.150873441113912	18.306426930423026	31.89561322965942	28.647086398803644
3	21.75757505317356	19.780952048877058	27.68938117210412	30.772091725845257
4	16.806332012227013	14.317150749466329	30.6616766815049	38.21484055680176
5	18.199498676956573	32.80217885687498	31.394290186233377	17.60403227993507
6	38.46666434213942	30.66671315721165	14.360929345994258	16.505693154654672
7	32.435290972698425	26.768868381392934	19.05996118039494	21.7358794655137
8	29.272384228857458	30.35367682097653	18.40521933851703	21.968719611648982
9	27.901300572995968	13.081664516478961	17.547081362327937	41.46995354819714
10	19.686033852865172	24.230872046397565	30.159578795662433	25.92351530507483
11	38.775051623875996	19.548886745158203	18.889495848781753	22.78656578218405
12	24.978207557038086	22.631597298899337	25.719731749555436	26.670463394507145
13	33.03811837267595	17.116656400004647	21.577036770146872	28.26818845717252
14	24.934041539301944	18.59699283658186	21.90518253350225	34.563783090613946
15	28.361556968351557	24.095662044731654	19.320308232313256	28.222472754603533
16	27.812193695107258	23.005071343615494	20.83861194729522	28.34412301398203
17	26.586780413533397	23.785337656954017	22.606027499157356	27.021854430355223
18	26.63675774939272	21.852105827977237	23.366922752085294	28.144213670544755
19	27.50574351941174	22.49251308515131	22.758284033984587	27.243459361452366
20	27.081904717628053	22.554887899673403	22.861725496577133	27.50148188612141
21	29.125551590945193	21.876125942886365	21.959034081443686	27.039288384724756
22	28.932615829255724	22.213569815238827	21.719607774768807	27.13420658073664
23	28.123292925301318	22.30965027487535	21.51776132529047	28.049295474532865
24	28.03960994432757	22.504910563814086	21.67544175703266	27.78003773482568
25	27.811031431482625	21.89627184571338	22.429750849420998	27.862945873382998
26	28.007841405254208	22.147320788634612	22.519245148517918	27.325592657593262
27	28.12639229496701	22.215119500071673	21.98770325085136	27.670784954109955
28	27.23416125245528	21.68590212965438	22.855914178453958	28.224022439436382
29	27.152027956314384	22.126400043391175	23.227451117129053	27.494120883165387
30	26.783590387304983	22.32553454441203	23.307647307228894	27.583227761054097
31	27.3465134028367	22.227129557526236	22.39139614980803	28.03496088982903
32	26.645281015973378	22.251149672435368	23.040714094770976	28.06285521682028
33	27.183021652971327	22.225192451485178	22.760221140025646	27.831564755517846
34	27.360073145124108	22.077972392364703	22.881483978195934	27.68047048431525
35	27.308546124431942	21.795154910370105	23.801996768907124	27.094302196290833
36	26.696420615457335	21.79864170124401	22.96942859246001	28.53550909083865
37	26.080420894400604	21.035421921066803	24.36956883893738	28.514588345595214
38	26.307837143620915	20.801032090098676	25.427228737355538	27.46390202892487
39	25.317588535431607	20.724710112080956	26.09088126702232	27.866820085465115
40	25.211047703173367	20.034325519047563	27.358910881499476	27.395715896279594
41	24.1630733349605	20.384554291271012	27.4255473293119	28.026825044456583
42	23.99725705784586	20.73400822107804	27.44026933522395	27.82846538585215
43	23.301835989105715	19.794124369956258	28.210075275940756	28.69396436499727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	7.5
2	9.0
3	12.5
4	16.0
5	16.0
6	26.0
7	36.0
8	36.0
9	36.0
10	50.5
11	65.0
12	65.0
13	124.0
14	183.0
15	271.0
16	359.0
17	350.0
18	341.0
19	341.0
20	437.0
21	533.0
22	558.5
23	584.0
24	743.5
25	903.0
26	903.0
27	1179.0
28	1455.0
29	1846.5
30	2238.0
31	2728.5
32	3219.0
33	3219.0
34	4069.0
35	4919.0
36	5848.5
37	6778.0
38	7687.5
39	8597.0
40	8597.0
41	9514.5
42	10432.0
43	11317.0
44	12202.0
45	13135.0
46	14068.0
47	14068.0
48	14997.5
49	15927.0
50	17181.0
51	18435.0
52	19843.0
53	21251.0
54	21251.0
55	21586.5
56	21922.0
57	21430.0
58	20938.0
59	19976.5
60	19015.0
61	19015.0
62	18675.5
63	18336.0
64	15969.5
65	13603.0
66	12279.0
67	10955.0
68	10955.0
69	9515.5
70	8076.0
71	7483.5
72	6891.0
73	6712.5
74	6534.0
75	6534.0
76	5932.5
77	5331.0
78	3681.5
79	2032.0
80	1462.5
81	893.0
82	893.0
83	711.5
84	530.0
85	389.0
86	248.0
87	191.5
88	135.0
89	135.0
90	100.5
91	66.0
92	41.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	258117.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.02943212926988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.81714096181138	65.41507091083128
2	5.121612545159795	7.378136864280092
3	1.388789717927127	3.0010120418777952
4	0.7095448038985586	2.044324371803493
5	0.4169115710169935	1.5014951854237906
6	0.29613729834213687	1.2798360859128175
7	0.2019324818209008	1.0181557395809517
8	0.14078969539128397	0.8112801446949656
9	0.10230892266717335	0.6632328241326495
>10	0.7010864800446324	9.41278886092489
>50	0.06826777867277968	3.3154920108112376
>100	0.03494020168291874	3.9125361169271935
>500	5.375415643525959E-4	0.24663884279887402
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	637	0.2467873096309038	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	295	0.11428925642247509	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	287	0.11118988675678085	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	261	0.10111693534327457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.8742120821177995E-4	0.0
16	0.0	0.0	0.0	3.8742120821177995E-4	3.8742120821177995E-4
17	3.8742120821177995E-4	0.0	0.0	7.748424164235599E-4	3.8742120821177995E-4
18	3.8742120821177995E-4	0.0	0.0	7.748424164235599E-4	3.8742120821177995E-4
19	3.8742120821177995E-4	0.0	0.0	0.0011622636246353397	3.8742120821177995E-4
20	3.8742120821177995E-4	0.0	0.0	0.0011622636246353397	7.748424164235599E-4
21	3.8742120821177995E-4	0.0	0.0	0.0011622636246353397	0.0011622636246353397
22	3.8742120821177995E-4	0.0	0.0	0.0019371060410588996	0.0011622636246353397
23	3.8742120821177995E-4	0.0	0.0	0.0030993696656942396	0.0011622636246353397
24	3.8742120821177995E-4	0.0	0.0	0.006198739331388479	0.0011622636246353397
25	3.8742120821177995E-4	0.0	0.0	0.008523266580659158	0.0011622636246353397
26	3.8742120821177995E-4	0.0	0.0	0.018983639202377216	0.0011622636246353397
27	3.8742120821177995E-4	0.0	0.0	0.04958991465110783	0.0011622636246353397
28	3.8742120821177995E-4	0.0	0.0	0.13172321079200516	0.0011622636246353397
29	3.8742120821177995E-4	0.0	0.0	0.24407536117342135	0.0011622636246353397
30	3.8742120821177995E-4	0.0	0.0	0.3974941596252862	0.0011622636246353397
31	3.8742120821177995E-4	0.0	0.0	0.76399462259363	0.0011622636246353397
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	20	0.0018392927	37.0	1
AGAGCGA	30	3.591816E-4	30.833334	10
GGTATCA	110	0.0	25.227272	1
ATTCCGA	45	0.003817895	20.555555	28
AATAACG	45	0.003817895	20.555555	2
TAATTTT	55	5.128257E-4	20.181818	14
TAGAAGT	65	6.875664E-5	19.923075	4
TATTAAA	60	9.2103845E-4	18.5	2
TAGAACT	50	0.0070204725	18.499998	4
CTCGCGG	50	0.0070204725	18.499998	22
TGCATCA	215	0.0	18.069767	14
TCCAGGA	300	0.0	16.65	2
CTGCATC	235	0.0	16.531914	13
CAGGACA	255	0.0	15.960784	4
TGAACAC	70	0.0025851978	15.857142	5
GCTGCAT	245	0.0	15.857142	12
ATCAAGC	235	0.0	15.744679	30
AAGAGGC	240	0.0	15.416667	22
CCAGGAC	300	0.0	15.416667	3
GCATCAG	240	0.0	15.416667	15
>>END_MODULE