FastQCFastQC Report
Fri 10 Feb 2017
ERR1632045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131434
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71660.6333555470314662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60410.533924205919214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50010.4420054550243319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.2137994792449228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.023.8958321
CTTATAC14300.020.05244837
ACCTATC656.902009E-519.9230777
CCGTATA609.236693E-418.52
GACCTAG650.001579913417.0769231
AAAGCCG1301.3929821E-817.07692315
TAGCCCC1251.6575359E-716.2799994
CGTCTAT700.002592460515.85714227
GGTCTAG957.059519E-515.5789481
GTATCAA33700.015.3709192
GTCCTAT2055.456968E-1215.3414631
GCGGTAT1752.237357E-914.824
GTATATA1001.0933398E-414.7999991
TTTAGAC1152.2096727E-514.4782613
TAACACT1152.2096727E-514.4782614
CGTATTG1956.730261E-1014.2307727
GTAATAT1304.4435255E-614.2307693
GCGACTA2353.6379788E-1214.17021415
AGTACCG1051.6555951E-414.0952385
TAGTACC1051.6555951E-414.0952384
Produced by FastQC (version 0.11.5)