##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131434
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61002851248946	34.0	31.0	34.0	31.0	34.0
2	32.77548668327096	34.0	31.0	34.0	31.0	34.0
3	32.84637283305964	34.0	31.0	34.0	31.0	34.0
4	36.30109224223419	37.0	37.0	37.0	35.0	37.0
5	36.161431422425	37.0	35.0	37.0	35.0	37.0
6	36.1933678853561	37.0	36.0	37.0	35.0	37.0
7	36.17784157096216	37.0	36.0	37.0	35.0	37.0
8	36.16846497453674	37.0	36.0	37.0	35.0	37.0
9	37.91428488095638	39.0	38.0	39.0	35.0	39.0
10	37.86995617950318	39.0	38.0	39.0	35.0	39.0
11	37.94164131535732	39.0	38.0	39.0	35.0	39.0
12	37.91470823751098	39.0	38.0	39.0	35.0	39.0
13	37.93347380403983	39.0	38.0	39.0	35.0	39.0
14	39.26234230189299	40.0	39.0	41.0	36.0	41.0
15	39.27063443382469	40.0	39.0	41.0	36.0	41.0
16	39.25559246054122	40.0	39.0	41.0	36.0	41.0
17	39.27761849122441	40.0	39.0	41.0	36.0	41.0
18	39.27596041837173	40.0	39.0	41.0	36.0	41.0
19	39.315339648622896	40.0	39.0	41.0	36.0	41.0
20	39.30170827463201	40.0	39.0	41.0	36.0	41.0
21	39.272571798266625	40.0	39.0	41.0	36.0	41.0
22	39.226914694096166	40.0	39.0	41.0	36.0	41.0
23	39.16633405041743	40.0	39.0	41.0	36.0	41.0
24	39.116974565021025	40.0	39.0	41.0	36.0	41.0
25	39.063050076274884	40.0	39.0	41.0	36.0	41.0
26	38.96272517884384	40.0	38.0	41.0	35.0	41.0
27	38.87527420954294	40.0	38.0	41.0	35.0	41.0
28	38.80283074399391	40.0	38.0	41.0	35.0	41.0
29	38.71570679332599	40.0	38.0	41.0	35.0	41.0
30	38.62200534896424	40.0	38.0	41.0	35.0	41.0
31	38.51887869729918	40.0	38.0	41.0	35.0	41.0
32	38.41164398453644	40.0	38.0	41.0	35.0	41.0
33	38.31526894189144	40.0	38.0	41.0	34.0	41.0
34	38.22543957491113	40.0	38.0	41.0	34.0	41.0
35	38.17436633511102	40.0	38.0	41.0	34.0	41.0
36	38.11131007199713	40.0	38.0	41.0	34.0	41.0
37	38.03887544478953	40.0	38.0	41.0	34.0	41.0
38	37.970412768221564	40.0	37.0	41.0	34.0	41.0
39	37.89162160585593	40.0	37.0	41.0	33.0	41.0
40	37.78141809420612	40.0	37.0	41.0	33.0	41.0
41	37.695473178285255	40.0	37.0	41.0	33.0	41.0
42	37.59407265470191	40.0	37.0	41.0	33.0	41.0
43	36.76724934905615	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	3.0
13	2.0
14	1.0
15	6.0
16	3.0
17	14.0
18	28.0
19	44.0
20	94.0
21	201.0
22	314.0
23	593.0
24	934.0
25	1492.0
26	2338.0
27	3387.0
28	5037.0
29	7228.0
30	9845.0
31	12865.0
32	16928.0
33	22655.0
34	32304.0
35	45889.0
36	72642.0
37	139100.0
38	303281.0
39	454204.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.8220594396138	19.63923657942045	13.773317754283502	24.765386226682246
2	18.504835456597558	21.601259993954574	34.96244588725458	24.931458662193286
3	19.708352409420257	22.984460428093907	31.090103355564707	26.217083806921128
4	14.850976725111673	16.615639975464763	34.319810081719304	34.21357321770426
5	14.98372861342332	36.05893052533334	33.32461283645356	15.63272802478978
6	32.67287353924312	35.1232153179063	16.49464308125794	15.709268061592635
7	27.91705039799052	31.368776260921983	21.45578089398056	19.258392447106946
8	26.821361210640653	33.15191164486837	20.636378259801276	19.390348884689697
9	26.458989211920446	14.609159703526675	21.12080775370017	37.811043330852705
10	17.38051004300737	27.320197201074038	32.466056349729634	22.833236406188963
11	34.68748508529884	22.272178492072893	21.67435307759887	21.365983345029406
12	21.630691670923802	26.867320586088095	28.71329657761743	22.788691165370672
13	30.287493570106612	20.054285093076572	25.345269808048897	24.31295152876792
14	22.725055107058832	21.991119234528924	26.01937010908281	29.264455549329433
15	25.325206772997806	28.01931000836107	23.115974948605043	23.539508270036077
16	24.146348792770944	26.805717346305663	24.98678667955886	24.061147181364532
17	22.87345085970547	27.253909640332534	26.16882646270132	23.70381303726068
18	22.684221969642067	25.09355384405984	28.199965707235243	24.022258479062852
19	23.363713658949617	26.183144575821483	28.124751421647222	22.328390343581685
20	24.13998518693976	25.113440112282287	27.90317420194196	22.84340049883599
21	24.746383792603016	25.478198463189194	26.851676721753105	22.923741022454692
22	24.713681929303874	25.572680333099413	26.6785336130963	23.035104124500414
23	23.74933049563651	25.59839990666711	26.95526208333849	23.697007514357885
24	23.509369525752273	26.07823346302126	27.224389579948987	23.188007431277477
25	23.415683106570953	25.226394115785812	27.339464785396235	24.018457992247008
26	23.219737077019076	26.0064661305918	27.26036162957804	23.51343516281109
27	23.656439527184087	25.567023794582806	27.081650365818955	23.694886312414155
28	23.00637951484576	25.85241383942855	27.660561729628064	23.480644916097624
29	22.687315389143333	25.998511623302818	28.015244371302263	23.298928616251587
30	22.524071222890598	26.732977796318654	27.751066345893793	22.99188463489695
31	23.328448676635137	26.254028074107726	26.98009782276297	23.437425426494165
32	22.292683444195596	25.984370277011298	27.525688639372692	24.197257639420418
33	22.346243793274727	25.617048807089056	28.176720869268557	23.859986530367657
34	23.178727172773666	25.072606974865526	28.043880597542586	23.704785254818223
35	22.847554519309124	25.25653286006961	28.280836531339876	23.615076089281388
36	22.228517085397822	25.3328077466295	28.20473841160863	24.233936756364045
37	22.627303050818696	24.348658428154007	28.528928775341733	24.495109745685564
38	21.89813988266218	24.16703051172229	29.487800437321134	24.447029168294396
39	22.018871626626034	23.7135352128361	30.136711465273276	24.130881695264595
40	21.34715767777882	23.710530176749153	30.647214066397154	24.295098079074872
41	20.735721217499208	23.95994817196584	31.25440812278931	24.04992248774564
42	20.89127602670593	23.803509528615898	31.13349961199681	24.17171483268136
43	19.913932231133234	23.538447669064215	31.291882690461836	25.25573740934071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	242.0
2	245.0
3	627.0
4	1009.0
5	1009.0
6	1355.5
7	1702.0
8	1731.5
9	1761.0
10	2552.5
11	3344.0
12	3344.0
13	5712.0
14	8080.0
15	11715.5
16	15351.0
17	14230.5
18	13110.0
19	13110.0
20	14497.0
21	15884.0
22	14386.5
23	12889.0
24	14647.5
25	16406.0
26	16406.0
27	18442.0
28	20478.0
29	24864.5
30	29251.0
31	32331.0
32	35411.0
33	35411.0
34	39340.5
35	43270.0
36	47124.0
37	50978.0
38	54651.5
39	58325.0
40	58325.0
41	62471.0
42	66617.0
43	67628.0
44	68639.0
45	71108.5
46	73578.0
47	73578.0
48	74914.5
49	76251.0
50	77053.5
51	77856.0
52	80302.5
53	82749.0
54	82749.0
55	78363.5
56	73978.0
57	70718.0
58	67458.0
59	62481.5
60	57505.0
61	57505.0
62	51831.0
63	46157.0
64	41490.5
65	36824.0
66	31558.0
67	26292.0
68	26292.0
69	22038.5
70	17785.0
71	15344.5
72	12904.0
73	10040.5
74	7177.0
75	7177.0
76	5643.0
77	4109.0
78	3415.0
79	2721.0
80	2322.0
81	1923.0
82	1923.0
83	1553.0
84	1183.0
85	1034.5
86	886.0
87	766.5
88	647.0
89	647.0
90	501.0
91	355.0
92	213.0
93	71.0
94	43.5
95	16.0
96	16.0
97	15.0
98	14.0
99	10.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1131434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.34765434040225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0982796686354	56.44167022773205
2	6.398179575403903	8.106193363010162
3	1.555373904053319	2.955878653321549
4	0.7108554988271326	1.8012411370270165
5	0.42695842182224203	1.3523407261659521
6	0.2870743763618974	1.0911293018256012
7	0.2053723784699685	0.9106900909667281
8	0.16042693084894902	0.8130135809848669
9	0.11278598292288557	0.6430254714577319
>10	0.8640784443476355	10.89845108458558
>50	0.11185800097197333	4.967553908582934
>100	0.06624132518906842	7.395194556429674
>500	0.0019564938910133535	0.7975493873083901
>1k	1.3974956364381095E-4	0.2141494025861911
>5k	4.192486909314329E-4	1.6119191080154476
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7166	0.6333555470314662	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6041	0.533924205919214	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5001	0.4420054550243319	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2419	0.2137994792449228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.838341432200198E-5	0.0
5	0.0	0.0	0.0	8.838341432200198E-5	0.0
6	0.0	0.0	0.0	8.838341432200198E-5	0.0
7	0.0	0.0	0.0	8.838341432200198E-5	0.0
8	0.0	0.0	0.0	8.838341432200198E-5	0.0
9	0.0	0.0	0.0	8.838341432200198E-5	0.0
10	0.0	0.0	0.0	8.838341432200198E-5	0.0
11	0.0	0.0	0.0	1.7676682864400397E-4	0.0
12	0.0	0.0	0.0	6.186839002540139E-4	0.0
13	0.0	0.0	0.0	7.070673145760159E-4	0.0
14	0.0	0.0	0.0	7.070673145760159E-4	0.0
15	0.0	0.0	0.0	8.838341432200199E-4	0.0
16	0.0	0.0	0.0	0.0021212019437280476	0.0
17	0.0	0.0	0.0	0.0032701863299140735	0.0
18	0.0	0.0	0.0	0.0038004868158460856	0.0
19	0.0	0.0	0.0	0.004772704373388108	0.0
20	0.0	0.0	0.0	0.004949471202032111	0.0
21	0.0	0.0	0.0	0.0061868390025401395	0.0
22	0.0	0.0	0.0	0.007600973631692171	0.0
23	0.0	0.0	0.0	0.01016409264703023	0.0
24	0.0	0.0	0.0	0.015290330677706344	0.0
25	0.0	0.0	0.0	0.01741153262143439	0.0
26	0.0	0.0	0.0	0.02306807113804252	0.0
27	0.0	0.0	0.0	0.05470933346531923	0.0
28	0.0	0.0	0.0	0.14795383557503133	0.0
29	0.0	0.0	0.0	0.27902643901456026	0.0
30	0.0	0.0	0.0	0.46754826176339054	0.0
31	0.0	0.0	0.0	1.0353233153679313	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2160	0.0	23.895832	1
CTTATAC	1430	0.0	20.052448	37
ACCTATC	65	6.902009E-5	19.923077	7
CCGTATA	60	9.236693E-4	18.5	2
GACCTAG	65	0.0015799134	17.076923	1
AAAGCCG	130	1.3929821E-8	17.076923	15
TAGCCCC	125	1.6575359E-7	16.279999	4
CGTCTAT	70	0.0025924605	15.857142	27
GGTCTAG	95	7.059519E-5	15.578948	1
GTATCAA	3370	0.0	15.370919	2
GTCCTAT	205	5.456968E-12	15.341463	1
GCGGTAT	175	2.237357E-9	14.8	24
GTATATA	100	1.0933398E-4	14.799999	1
TTTAGAC	115	2.2096727E-5	14.478261	3
TAACACT	115	2.2096727E-5	14.478261	4
CGTATTG	195	6.730261E-10	14.23077	27
GTAATAT	130	4.4435255E-6	14.230769	3
GCGACTA	235	3.6379788E-12	14.170214	15
AGTACCG	105	1.6555951E-4	14.095238	5
TAGTACC	105	1.6555951E-4	14.095238	4
>>END_MODULE