FastQCFastQC Report
Fri 10 Feb 2017
ERR1632044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422607
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.14386889000892084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT200.001840619537.01
GGTATCA2200.029.4318181
ATAACGA358.860077E-426.42857212
GTATTAA601.334065E-624.6666681
GATAACG400.001929177923.12511
TCCGATA400.001929177923.1258
TTCCGAT400.001929177923.1257
CCGATAA450.00382186520.5555579
TAACGAA450.00382186520.55555713
GTACTGG450.00382186520.5555571
CGATAAC450.00382186520.55555710
GACCGTG656.888938E-519.9230777
TACTGGA1052.2493623E-819.380952
GTATCAA3300.019.0606062
ATAATAC500.007027700518.53
ATTCCGA500.007027700518.56
GTGTCTA500.007027700518.523
TATACTG500.007027700518.55
ACGAACG500.007027700518.515
GTAGCGC1054.77954E-717.6190473
Produced by FastQC (version 0.11.5)