FastQCFastQC Report
Fri 10 Feb 2017
ERR1632043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808525
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.10958226399925791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAG400.001930694623.1253
GGTATCA3650.022.301371
TAAGGAC555.1412795E-420.1818183
CTTATAC7800.019.21153837
GTATTGA500.00703312218.51
TACTCCG500.00703312218.55
GTATAAT902.1506094E-618.51
TAGGACG752.0663106E-417.2666664
TTTCGTC904.443333E-516.44444517
GTATCAA5000.015.912
TACGACG700.00259160215.8571425
TACCGTC700.00259160215.8571427
TAGGGAG1201.9336749E-615.4166665
ATCAACG7700.015.3766232
TTGTATA855.361706E-415.2352942
TAACACT855.361706E-415.2352944
TCAACGC7950.015.1257863
CGACGGT750.004103005414.87
CGGATAG1001.09272674E-414.820
GTCACTA1001.09272674E-414.821
Produced by FastQC (version 0.11.5)