##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808525
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70925698030364	34.0	31.0	34.0	31.0	34.0
2	32.886980612844376	34.0	31.0	34.0	31.0	34.0
3	32.975414489347884	34.0	31.0	34.0	31.0	34.0
4	36.38479082279459	37.0	37.0	37.0	35.0	37.0
5	36.24726260783525	37.0	37.0	37.0	35.0	37.0
6	36.2701771744844	37.0	37.0	37.0	35.0	37.0
7	36.24924399369222	37.0	37.0	37.0	35.0	37.0
8	36.2310392381188	37.0	36.0	37.0	35.0	37.0
9	38.012310070807956	39.0	38.0	39.0	35.0	39.0
10	37.97587829689867	39.0	38.0	39.0	35.0	39.0
11	38.04054914814013	39.0	38.0	39.0	35.0	39.0
12	38.00832379951146	39.0	38.0	39.0	35.0	39.0
13	38.023340032775735	39.0	38.0	39.0	35.0	39.0
14	39.42286880430414	40.0	39.0	41.0	37.0	41.0
15	39.434099131133856	40.0	39.0	41.0	37.0	41.0
16	39.43252836956186	40.0	39.0	41.0	37.0	41.0
17	39.41668841408738	40.0	39.0	41.0	37.0	41.0
18	39.40969048576111	40.0	39.0	41.0	37.0	41.0
19	39.43314801644971	40.0	39.0	41.0	37.0	41.0
20	39.401003061129835	40.0	39.0	41.0	37.0	41.0
21	39.37814538820692	40.0	39.0	41.0	37.0	41.0
22	39.33942673386723	40.0	39.0	41.0	36.0	41.0
23	39.29298661142204	40.0	39.0	41.0	36.0	41.0
24	39.24975727404842	40.0	39.0	41.0	36.0	41.0
25	39.19801861414304	40.0	39.0	41.0	36.0	41.0
26	39.11624254042856	40.0	39.0	41.0	36.0	41.0
27	39.03685352957546	40.0	39.0	41.0	36.0	41.0
28	38.98545004792678	40.0	39.0	41.0	35.0	41.0
29	38.916590086886615	40.0	38.0	41.0	35.0	41.0
30	38.85652639064964	40.0	38.0	41.0	35.0	41.0
31	38.77656102161343	40.0	38.0	41.0	35.0	41.0
32	38.69729198231347	40.0	38.0	41.0	35.0	41.0
33	38.62458922111252	40.0	38.0	41.0	35.0	41.0
34	38.552548158684026	40.0	38.0	41.0	35.0	41.0
35	38.53022973934016	40.0	38.0	41.0	35.0	41.0
36	38.496574626634924	40.0	38.0	41.0	35.0	41.0
37	38.44173031136947	40.0	38.0	41.0	34.0	41.0
38	38.398653102872515	40.0	38.0	41.0	34.0	41.0
39	38.35103552765839	40.0	38.0	41.0	34.0	41.0
40	38.27792337899261	40.0	38.0	41.0	34.0	41.0
41	38.23329890850623	40.0	38.0	41.0	34.0	41.0
42	38.179868278655576	40.0	37.0	41.0	34.0	41.0
43	37.32138152809128	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	3.0
17	1.0
18	9.0
19	23.0
20	45.0
21	89.0
22	178.0
23	288.0
24	492.0
25	713.0
26	1216.0
27	1792.0
28	2661.0
29	3872.0
30	5190.0
31	7503.0
32	10181.0
33	13869.0
34	20016.0
35	30050.0
36	48150.0
37	87775.0
38	212751.0
39	361652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01573853622337	17.759624006678827	12.107850715809654	27.116786741288145
2	19.513682322748213	19.62623295507251	33.29173494944498	27.5683497727343
3	20.101295569091864	21.06044958411923	28.88989208744318	29.94836275934572
4	15.42129185863146	15.144367830308278	32.98599301196624	36.44834729909403
5	16.74196839924554	33.97495439225751	32.77016789833338	16.51290931016357
6	36.32058377910393	33.2949506817971	15.102563309730682	15.281902229368294
7	31.16984632509817	28.781670325592902	20.22473021860796	19.82375313070097
8	28.36758294425033	31.99381589932284	19.204477288890264	20.434123867536563
9	27.36575863455057	13.796233882687611	18.69602053121425	40.14198695154757
10	17.950217989548868	25.33242633190068	31.83117405151356	24.886181627036887
11	38.0929470331777	20.308586623790234	20.43239231934696	21.166074023685105
12	23.33384867505643	23.796790451748553	27.741133545654122	25.12822732754089
13	32.04130979252342	18.336600599857768	22.735722457561607	26.886367150057204
14	23.85689991033054	19.15896230790637	23.55053956278408	33.43359821897901
15	26.741164466157507	25.96567824124177	20.670480195417582	26.62267709718314
16	26.767879781082836	24.366222442101357	22.5137132432516	26.352184533564206
17	25.490862991249497	24.658730404130978	23.92455397173866	25.925852632880865
18	25.880708697937603	23.696979066819207	24.475062614019354	25.947249621223833
19	26.27847005349247	23.86036300670975	24.336662440864536	25.524504498933243
20	26.457561609102996	23.659750780742712	24.00593673665007	25.87675087350422
21	26.951918617235087	23.634643331993445	23.532729352833865	25.880708697937603
22	27.113076280881852	23.598775548065923	23.40645001700628	25.881698154045946
23	26.49664512538264	23.449491357719303	23.721220741473672	26.332642775424386
24	26.2353050307659	23.88225472310689	23.43452583408058	26.44791441204663
25	26.521381528091275	23.098976531337932	23.829195139296868	26.550446801273925
26	26.067221174360718	23.732352122692557	23.926161837914723	26.274264865032006
27	26.07550786926811	23.54955010667574	23.81064283726539	26.564299186790763
28	25.991527782072293	23.717262917040287	24.03277573358894	26.25843356729848
29	25.56061964688785	23.940632633499273	24.342475495501066	26.15627222411181
30	25.61256609257599	24.144584273831978	23.92950125228039	26.313348381311645
31	26.213660678395843	23.88336786122878	23.621533038557867	26.281438421817505
32	25.478000061841005	23.754738567143875	24.02646795089824	26.74079342011688
33	25.43471135710089	23.457283324572526	24.58798429238428	26.520021025942302
34	25.970378157756407	23.06236665532915	24.44055533224081	26.526699854673634
35	25.537367428341735	23.14869670078229	25.11561176215949	26.19832410871649
36	25.37633344670851	23.058285148882224	24.966451253826413	26.59893015058285
37	25.156426826628735	22.336229553817137	25.68578584459355	26.821557774960574
38	24.462694412665037	22.338703194088	26.49553198726075	26.703070405986207
39	24.450202529297176	21.859682755635262	26.964286818589407	26.725827896478155
40	24.04662811910578	21.818125599084752	27.740762499613492	26.394483782195977
41	23.49772734300114	21.72437463281902	28.274326706038778	26.503571318141063
42	23.148820382795833	22.0041433474537	28.465662780990076	26.381373488760396
43	22.610803623882997	21.49679972789957	28.84190346618843	27.050493182029
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	33.0
2	43.0
3	101.0
4	159.0
5	159.0
6	185.0
7	211.0
8	209.5
9	208.0
10	306.0
11	404.0
12	404.0
13	714.0
14	1024.0
15	1492.5
16	1961.0
17	2030.5
18	2100.0
19	2100.0
20	2477.5
21	2855.0
22	3131.0
23	3407.0
24	4174.5
25	4942.0
26	4942.0
27	6307.5
28	7673.0
29	9394.0
30	11115.0
31	13232.5
32	15350.0
33	15350.0
34	18420.5
35	21491.0
36	24536.0
37	27581.0
38	30745.0
39	33909.0
40	33909.0
41	37413.5
42	40918.0
43	43659.5
44	46401.0
45	49478.5
46	52556.0
47	52556.0
48	54851.5
49	57147.0
50	59117.5
51	61088.0
52	62287.0
53	63486.0
54	63486.0
55	62987.0
56	62488.0
57	60954.5
58	59421.0
59	57724.5
60	56028.0
61	56028.0
62	51949.5
63	47871.0
64	43016.0
65	38161.0
66	33663.5
67	29166.0
68	29166.0
69	24942.0
70	20718.0
71	17828.5
72	14939.0
73	12052.0
74	9165.0
75	9165.0
76	7534.5
77	5904.0
78	4792.0
79	3680.0
80	3043.0
81	2406.0
82	2406.0
83	1796.5
84	1187.0
85	966.0
86	745.0
87	561.5
88	378.0
89	378.0
90	277.5
91	177.0
92	104.0
93	31.0
94	19.0
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	808525.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.9659275339396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90427120637277	65.75886881508282
2	6.621407071970488	9.79517031316169
3	1.791481835255324	3.9752584681459333
4	0.821897620135095	2.431696792449195
5	0.4440922471338333	1.6423847484942757
6	0.27321725708272543	1.2125260703041572
7	0.18905575305501543	0.9788578891239158
8	0.13619246026816062	0.8058881317490975
9	0.11359354097878736	0.7561846458324527
>10	0.6487847137763233	8.754373135248288
>50	0.041754346589079956	2.060508098657489
>100	0.013748937486113373	1.5493649588890763
>500	5.030098962410144E-4	0.27891793286151606
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.10958226399925791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.2368201354318048E-4	0.0
12	0.0	0.0	0.0	1.2368201354318048E-4	0.0
13	0.0	0.0	0.0	1.2368201354318048E-4	0.0
14	0.0	0.0	0.0	1.2368201354318048E-4	0.0
15	0.0	0.0	0.0	2.4736402708636096E-4	0.0
16	0.0	0.0	0.0	2.4736402708636096E-4	0.0
17	0.0	0.0	0.0	2.4736402708636096E-4	0.0
18	0.0	1.2368201354318048E-4	0.0	3.7104604062954144E-4	0.0
19	0.0	1.2368201354318048E-4	0.0	6.184100677159024E-4	0.0
20	0.0	1.2368201354318048E-4	0.0	7.420920812590829E-4	0.0
21	0.0	1.2368201354318048E-4	0.0	7.420920812590829E-4	0.0
22	0.0	2.4736402708636096E-4	0.0	0.001236820135431805	0.0
23	0.0	2.4736402708636096E-4	0.0	0.0022262762437772485	0.0
24	0.0	2.4736402708636096E-4	0.0	0.004328870474011317	0.0
25	0.0	2.4736402708636096E-4	0.0	0.005689372622986302	0.0
26	0.0	2.4736402708636096E-4	0.0	0.008410376920936272	0.0
27	0.0	2.4736402708636096E-4	0.0	0.02807581707430197	0.0
28	0.0	2.4736402708636096E-4	0.0	0.10438761943044433	0.0
29	0.0	2.4736402708636096E-4	0.0	0.2060542345629387	0.0
30	0.0	2.4736402708636096E-4	0.0	0.343341269595869	0.0
31	0.0	2.4736402708636096E-4	0.0	0.7660863918864599	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATAG	40	0.0019306946	23.125	3
GGTATCA	365	0.0	22.30137	1
TAAGGAC	55	5.1412795E-4	20.181818	3
CTTATAC	780	0.0	19.211538	37
GTATTGA	50	0.007033122	18.5	1
TACTCCG	50	0.007033122	18.5	5
GTATAAT	90	2.1506094E-6	18.5	1
TAGGACG	75	2.0663106E-4	17.266666	4
TTTCGTC	90	4.443333E-5	16.444445	17
GTATCAA	500	0.0	15.91	2
TACGACG	70	0.002591602	15.857142	5
TACCGTC	70	0.002591602	15.857142	7
TAGGGAG	120	1.9336749E-6	15.416666	5
ATCAACG	770	0.0	15.376623	2
TTGTATA	85	5.361706E-4	15.235294	2
TAACACT	85	5.361706E-4	15.235294	4
TCAACGC	795	0.0	15.125786	3
CGACGGT	75	0.0041030054	14.8	7
CGGATAG	100	1.09272674E-4	14.8	20
GTCACTA	100	1.09272674E-4	14.8	21
>>END_MODULE