FastQCFastQC Report
Fri 10 Feb 2017
ERR1632042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771576
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10040.1301232801434985No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA9340.12105093989444982No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG7920.102647049674951No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC7860.10186942051074685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.024.412371
TAGGATA400.001930614723.1250024
CTTATAC6700.020.15671737
ATTAGAG609.2330656E-418.53
GTACTAT801.6155478E-518.51
TACTATA609.2330656E-418.52
TATAGGA500.007032835418.52
TCGCCTA752.0661566E-417.26666635
GTGTTAG904.4429078E-516.4444451
GTATCAA7350.016.1088452
GCGTTAT700.00259145815.8571421
ATACTGC700.00259145815.8571426
CGCAATT855.361312E-415.23529412
CCTTACA750.004102779614.832
ATCTATT750.004102779614.88
CGCGGTT1001.0926234E-414.810
GTCTAGA750.004102779614.81
CCGCTCA750.004102779614.89
AATGACG1001.0926234E-414.87
TTTTCGG1152.207873E-514.4782629
Produced by FastQC (version 0.11.5)