##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	771576
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74931309423829	34.0	31.0	34.0	31.0	34.0
2	32.922647930988006	34.0	31.0	34.0	31.0	34.0
3	32.99437514904559	34.0	33.0	34.0	31.0	34.0
4	36.39135742946903	37.0	37.0	37.0	35.0	37.0
5	36.2637990295188	37.0	37.0	37.0	35.0	37.0
6	36.29618987630512	37.0	37.0	37.0	35.0	37.0
7	36.27627997760428	37.0	37.0	37.0	35.0	37.0
8	36.263594253838896	37.0	37.0	37.0	35.0	37.0
9	38.05009098261221	39.0	38.0	39.0	35.0	39.0
10	38.021509222681885	39.0	38.0	39.0	35.0	39.0
11	38.084311850031625	39.0	38.0	39.0	37.0	39.0
12	38.05384045123228	39.0	38.0	39.0	35.0	39.0
13	38.06179430153348	39.0	38.0	39.0	35.0	39.0
14	39.46491596420832	40.0	39.0	41.0	37.0	41.0
15	39.47951335966904	40.0	39.0	41.0	37.0	41.0
16	39.47103979387643	40.0	39.0	41.0	37.0	41.0
17	39.462130755751865	40.0	39.0	41.0	37.0	41.0
18	39.44919489460533	40.0	39.0	41.0	37.0	41.0
19	39.4864977656122	41.0	39.0	41.0	37.0	41.0
20	39.464446794612584	40.0	39.0	41.0	37.0	41.0
21	39.43697704438707	40.0	39.0	41.0	37.0	41.0
22	39.403988719192924	40.0	39.0	41.0	37.0	41.0
23	39.353354692214374	40.0	39.0	41.0	36.0	41.0
24	39.3146831938785	40.0	39.0	41.0	36.0	41.0
25	39.26835464037243	40.0	39.0	41.0	36.0	41.0
26	39.18292559644157	40.0	39.0	41.0	36.0	41.0
27	39.10885382645391	40.0	39.0	41.0	36.0	41.0
28	39.048842887803666	40.0	39.0	41.0	36.0	41.0
29	38.99746492892469	40.0	39.0	41.0	35.0	41.0
30	38.93379524505687	40.0	38.0	41.0	35.0	41.0
31	38.86532499714869	40.0	38.0	41.0	35.0	41.0
32	38.7896202059162	40.0	38.0	41.0	35.0	41.0
33	38.7277299967858	40.0	38.0	41.0	35.0	41.0
34	38.658213837651765	40.0	38.0	41.0	35.0	41.0
35	38.62973057741558	40.0	38.0	41.0	35.0	41.0
36	38.59788147894699	40.0	38.0	41.0	35.0	41.0
37	38.558451792176015	40.0	38.0	41.0	35.0	41.0
38	38.504602268603485	40.0	38.0	41.0	35.0	41.0
39	38.45735611268365	40.0	38.0	41.0	34.0	41.0
40	38.39651959107074	40.0	38.0	41.0	34.0	41.0
41	38.35009253787054	40.0	38.0	41.0	34.0	41.0
42	38.30805131315645	40.0	38.0	41.0	34.0	41.0
43	37.45139558514003	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	0.0
16	1.0
17	5.0
18	11.0
19	24.0
20	49.0
21	99.0
22	164.0
23	284.0
24	477.0
25	731.0
26	1133.0
27	1653.0
28	2399.0
29	3427.0
30	4891.0
31	6658.0
32	8940.0
33	12633.0
34	18145.0
35	27277.0
36	43341.0
37	79537.0
38	196922.0
39	362769.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.81833545885305	17.972435638226177	12.676392215413648	26.532836687507128
2	19.618935788567814	20.4543687206445	33.55962342011674	26.36707207067094
3	20.639962881167897	21.65632419878275	29.61380343608407	28.089909483965286
4	15.926104492622892	15.580189119412735	32.650834136883475	35.842872251080905
5	16.330471658009063	34.51571847750578	33.08151108899188	16.072298775493277
6	35.62371043163603	33.07010586125022	15.433606021960248	15.872577685153505
7	29.683012431698234	29.086441257892936	20.682862090059825	20.547684220348998
8	26.926317044594438	32.40432569183075	20.049612740676224	20.619744522898586
9	26.327672193017925	14.52663120677678	19.568908312337346	39.57678828786795
10	17.99706056175931	26.10436301803063	32.519155598411565	23.3794208217985
11	36.54442336205377	21.04134913475795	20.856662208259458	21.55756529492882
12	22.301626800211515	24.640216906694867	27.783523593268843	25.27463269982477
13	30.930588820803134	19.032733003618567	23.959661783155514	26.07701639242278
14	23.740111149128538	20.54574014743849	23.326542038632617	32.38760666480036
15	26.399473285846113	26.85568239551256	21.461268883428204	25.283575435213123
16	26.32676495899302	25.270874158864455	22.888348004603564	25.51401287753896
17	25.05041629081257	25.673426856200816	23.952663120677677	25.32349373230894
18	24.31957448132135	24.428442564309933	25.686128132549484	25.56585482181924
19	25.790330440552843	24.66069447468558	25.236658475639473	24.31231660912211
20	25.817547461299988	23.955644032473796	25.362115980797746	24.864692525428474
21	25.897384055491617	24.716294959926177	24.121538254170684	25.264782730411522
22	25.969703567762604	24.09289557995583	24.170140076933443	25.767260775348117
23	24.989372401422543	24.683893744751003	24.523442927203543	25.803290926622914
24	26.198844961481438	24.667433927442016	24.137090837454767	24.996630273621783
25	25.993939676713634	23.881121237570895	24.43686688025548	25.688072205459996
26	25.83672898068369	24.561805965970947	24.196061049073585	25.405404004271777
27	25.70362478874408	23.98506433585285	24.320870529928353	25.99044034547472
28	24.84538140118407	24.39785581718457	25.215792093066657	25.540970688564702
29	24.999222370835795	24.7584165396539	24.900074652399766	25.342286437110538
30	24.7019088203884	25.437416404864848	24.753750764668677	25.106924010078075
31	25.635841446597613	24.388653872074816	24.750899457733265	25.224605223594303
32	24.525905419556853	24.342903356247472	24.98898358684044	26.14220763735523
33	24.823737389447054	23.844443061992596	25.149564009248603	26.182255539311743
34	25.414217134799422	23.859477225833878	25.02579136727944	25.70051427208726
35	25.294721453233382	23.654831150787476	25.397368502908336	25.65307889307081
36	24.61831368523645	23.9566808713594	25.64465457712526	25.78035086627889
37	25.36911464327558	22.412698165832012	25.915917550571816	26.302269640320592
38	24.171954544983254	22.898327578877517	27.215206279096293	25.714511597042932
39	24.0494779516211	22.321715553620123	27.810092589712486	25.81871390504629
40	23.691898140947877	22.024402003172728	28.55195081236327	25.73174904351613
41	22.835210011716278	22.58585025972814	29.004919800512198	25.574019928043377
42	23.213008180658807	22.2430454031748	28.568669839393657	25.97527657677274
43	22.170855495764513	22.43926716227565	28.95100417846071	26.43887316349912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	37.0
2	38.0
3	100.0
4	162.0
5	162.0
6	201.0
7	240.0
8	243.0
9	246.0
10	346.5
11	447.0
12	447.0
13	737.0
14	1027.0
15	1652.5
16	2278.0
17	2286.5
18	2295.0
19	2295.0
20	2786.0
21	3277.0
22	3906.5
23	4536.0
24	5275.0
25	6014.0
26	6014.0
27	7032.5
28	8051.0
29	11219.0
30	14387.0
31	15715.5
32	17044.0
33	17044.0
34	19561.0
35	22078.0
36	24619.0
37	27160.0
38	32190.0
39	37220.0
40	37220.0
41	41308.5
42	45397.0
43	44851.5
44	44306.0
45	48005.0
46	51704.0
47	51704.0
48	54735.5
49	57767.0
50	61041.5
51	64316.0
52	67552.0
53	70788.0
54	70788.0
55	63427.0
56	56066.0
57	53160.0
58	50254.0
59	46844.5
60	43435.0
61	43435.0
62	40544.0
63	37653.0
64	33990.5
65	30328.0
66	26909.5
67	23491.0
68	23491.0
69	20214.0
70	16937.0
71	14918.0
72	12899.0
73	10411.5
74	7924.0
75	7924.0
76	6312.5
77	4701.0
78	3839.5
79	2978.0
80	2416.5
81	1855.0
82	1855.0
83	1438.0
84	1021.0
85	841.5
86	662.0
87	506.5
88	351.0
89	351.0
90	263.0
91	175.0
92	98.0
93	21.0
94	14.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	771576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.60815692281294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.75420549411571	53.79228814146951
2	6.279932337657796	7.612302491708224
3	1.7228208214464606	3.132509840883498
4	0.7759134159759716	1.8810672829595012
5	0.498922478081462	1.5119385921939974
6	0.3098153459354619	1.1266402262131232
7	0.2248684108634693	0.9540201952817703
8	0.1666771944377588	0.8081598044750313
9	0.1234571016135811	0.6734256649048429
>10	0.9220713866253767	11.091242439034499
>50	0.12395442543879731	5.3248762299053345
>100	0.09350143675000579	10.484376942682703
>500	0.003645698221570382	1.4766563329316817
>1k	2.1445283656296366E-4	0.1304958153563426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1004	0.1301232801434985	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	934	0.12105093989444982	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	792	0.102647049674951	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	786	0.10186942051074685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.296048607006957E-4	0.0	0.0	0.0
11	0.0	1.296048607006957E-4	0.0	0.0	0.0
12	0.0	1.296048607006957E-4	0.0	0.0	0.0
13	0.0	1.296048607006957E-4	0.0	0.0	0.0
14	0.0	1.296048607006957E-4	0.0	1.296048607006957E-4	0.0
15	0.0	1.296048607006957E-4	0.0	2.592097214013914E-4	0.0
16	0.0	1.296048607006957E-4	0.0	3.8881458210208715E-4	0.0
17	0.0	1.296048607006957E-4	0.0	6.480243035034786E-4	0.0
18	0.0	1.296048607006957E-4	0.0	7.776291642041743E-4	0.0
19	0.0	1.296048607006957E-4	0.0	0.0011664437463062614	0.0
20	0.0	1.296048607006957E-4	0.0	0.0015552583284083486	0.0
21	0.0	1.296048607006957E-4	0.0	0.0018144680498097402	0.0
22	0.0	1.296048607006957E-4	0.0	0.0025920972140139145	0.0
23	0.0	1.296048607006957E-4	0.0	0.0037585409603201757	0.0
24	0.0	1.296048607006957E-4	0.0	0.0067394527564361775	0.0
25	0.0	1.296048607006957E-4	0.0	0.009201945109749397	0.0
26	0.0	1.296048607006957E-4	0.0	0.015293373562682094	0.0
27	0.0	1.296048607006957E-4	0.0	0.04160316028492333	0.0
28	0.0	1.296048607006957E-4	0.0	0.12066212531234771	0.0
29	0.0	1.296048607006957E-4	0.0	0.2174769562557674	0.0
30	0.0	1.296048607006957E-4	0.0	0.3597830933051313	0.0
31	0.0	1.296048607006957E-4	0.0	0.725787219923896	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	485	0.0	24.41237	1
TAGGATA	40	0.0019306147	23.125002	4
CTTATAC	670	0.0	20.156717	37
ATTAGAG	60	9.2330656E-4	18.5	3
GTACTAT	80	1.6155478E-5	18.5	1
TACTATA	60	9.2330656E-4	18.5	2
TATAGGA	50	0.0070328354	18.5	2
TCGCCTA	75	2.0661566E-4	17.266666	35
GTGTTAG	90	4.4429078E-5	16.444445	1
GTATCAA	735	0.0	16.108845	2
GCGTTAT	70	0.002591458	15.857142	1
ATACTGC	70	0.002591458	15.857142	6
CGCAATT	85	5.361312E-4	15.235294	12
CCTTACA	75	0.0041027796	14.8	32
ATCTATT	75	0.0041027796	14.8	8
CGCGGTT	100	1.0926234E-4	14.8	10
GTCTAGA	75	0.0041027796	14.8	1
CCGCTCA	75	0.0041027796	14.8	9
AATGACG	100	1.0926234E-4	14.8	7
TTTTCGG	115	2.207873E-5	14.47826	29
>>END_MODULE