FastQCFastQC Report
Fri 10 Feb 2017
ERR1632041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83941
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16291.9406487890303903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14031.6714120632348912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10381.2365828379457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.6039956636208765No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT4090.4872469949130937No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT3890.46342073599313804RNA PCR Primer, Index 46 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1920.22873208563157454No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.2251581467935812No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1670.19894926198162996No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1650.19656663608963437No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA1650.19656663608963437No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1580.18822744546764988No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.16320987360169645No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT1370.16320987360169645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.14653149235772744No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA1230.14653149235772744No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG1220.14534017941172966No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA1170.13938361468174074No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT1160.13819230173574296No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1020.12151392049177398No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT980.11674866870778286No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.11436604281578727No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTAC900.10721816513980058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGA551.8128048E-830.27272813
TATGCCG801.8189894E-1230.062533
CCGTCTT801.8189894E-1230.062537
GCCGTCT801.8189894E-1230.062536
GTGTAGA250.005467710629.5999989
CCTGGAT250.005467710629.5999983
ATGCCGT900.028.77777934
GCCTTAT405.8757465E-527.7526
TTCCTCG405.8757465E-527.7520
GTATGCC950.027.26315932
GGCAGTC556.146256E-726.909098
CTTATTG556.146256E-726.9090928
CAGTCGG556.146256E-726.9090910
CTACTAG358.8007643E-426.428571
CGGTGAT659.1766196E-825.61538514
CGTATGC959.094947E-1225.3157931
TGCCGTC959.094947E-1225.3157935
GCAGTCG601.312892E-624.6666689
TCCTCGC451.3089854E-424.66666821
TTATTGA601.312892E-624.66666829
Produced by FastQC (version 0.11.5)