##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632041.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83941
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55312660082677	33.0	31.0	34.0	31.0	34.0
2	32.68281292812809	34.0	31.0	34.0	31.0	34.0
3	32.71594334115629	34.0	31.0	34.0	31.0	34.0
4	36.21110065403081	37.0	35.0	37.0	35.0	37.0
5	36.10869539319284	37.0	35.0	37.0	35.0	37.0
6	36.1718707187191	37.0	36.0	37.0	35.0	37.0
7	36.15967167415209	37.0	35.0	37.0	35.0	37.0
8	36.166188155966694	37.0	36.0	37.0	35.0	37.0
9	37.90535018644048	39.0	38.0	39.0	35.0	39.0
10	37.83232270285081	39.0	37.0	39.0	35.0	39.0
11	37.923029270559084	39.0	38.0	39.0	35.0	39.0
12	37.87635362933489	39.0	38.0	39.0	35.0	39.0
13	37.93883799335247	39.0	38.0	39.0	35.0	39.0
14	39.15982654483506	40.0	38.0	41.0	36.0	41.0
15	39.174110387057574	40.0	39.0	41.0	36.0	41.0
16	39.144899393621714	40.0	38.0	41.0	36.0	41.0
17	39.200700492012245	40.0	39.0	41.0	36.0	41.0
18	39.280720982594914	40.0	39.0	41.0	36.0	41.0
19	39.32869515493025	40.0	39.0	41.0	36.0	41.0
20	39.323441464838396	40.0	39.0	41.0	36.0	41.0
21	39.30090182390012	40.0	39.0	41.0	36.0	41.0
22	39.227242944449074	40.0	39.0	41.0	36.0	41.0
23	39.169094959554926	40.0	39.0	41.0	36.0	41.0
24	39.11948868848358	40.0	39.0	41.0	35.0	41.0
25	39.03117665979676	40.0	38.0	41.0	35.0	41.0
26	38.88115462050726	40.0	38.0	41.0	35.0	41.0
27	38.73538556843497	40.0	38.0	41.0	35.0	41.0
28	38.644643261338324	40.0	38.0	41.0	35.0	41.0
29	38.54221417424143	40.0	38.0	41.0	35.0	41.0
30	38.366257252117556	40.0	37.0	41.0	35.0	41.0
31	38.12235975268343	40.0	37.0	41.0	34.0	41.0
32	37.94033904766443	40.0	37.0	41.0	34.0	41.0
33	37.75760355487783	40.0	36.0	41.0	33.0	41.0
34	37.60849882655675	40.0	35.0	41.0	33.0	41.0
35	37.501292574546405	40.0	35.0	41.0	33.0	41.0
36	37.345373536174215	40.0	35.0	41.0	33.0	41.0
37	37.17178732681288	40.0	35.0	41.0	33.0	41.0
38	37.03981367865524	40.0	35.0	41.0	33.0	41.0
39	36.85469555997665	39.0	35.0	41.0	32.0	41.0
40	36.6216390083511	39.0	35.0	41.0	31.0	41.0
41	36.401115068917456	39.0	35.0	41.0	31.0	41.0
42	36.17112019156312	39.0	35.0	41.0	30.0	41.0
43	35.30084225825282	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	3.0
15	1.0
16	0.0
17	0.0
18	3.0
19	2.0
20	7.0
21	20.0
22	38.0
23	66.0
24	104.0
25	128.0
26	205.0
27	354.0
28	485.0
29	740.0
30	998.0
31	1237.0
32	1541.0
33	2089.0
34	2752.0
35	3930.0
36	5996.0
37	13179.0
38	21880.0
39	28181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.104573450399684	20.417912581456022	15.207109755661715	22.27040421248258
2	18.489176921885612	22.00712405141707	34.686267735671485	24.81743129102584
3	18.99071967215068	24.981832477573533	33.53188549099963	22.495562359276157
4	12.751813773960283	18.73577870170715	34.75655519948535	33.75585232484721
5	12.04893913582159	39.04885574391537	34.1418377193505	14.760367400912546
6	28.434257395075115	36.962866775473245	17.296672662941827	17.30620316650981
7	25.603697835384377	33.63910365613943	21.3983631360122	19.358835372463993
8	29.48856935228315	32.282198210647955	19.330243861760046	18.89898857530885
9	26.76522795773222	13.084190085893663	21.899905886277267	38.25067607009686
10	19.163460049320356	27.975601910865965	29.69466649194077	23.16627154787291
11	32.31555497313589	23.425977770100427	21.210135690544547	23.04833156621913
12	20.3762166283461	31.47210540736946	27.18933536650743	20.96234259777701
13	31.095650516434166	20.224919884204382	26.089753517351472	22.589676082009984
14	21.40908495252618	23.69878843473392	29.027531242182008	25.86459537055789
15	26.58891364172454	28.039932809949846	24.5160291156884	20.855124432637208
16	21.84391417781537	26.34826842663299	28.124516029115686	23.68330136643595
17	19.330243861760046	30.66677785587496	27.802861533696287	22.20011674866871
18	19.345730930058018	25.98968322988766	30.57147282019514	24.093113019859185
19	21.138656913784683	28.274621460311412	32.36797274275979	18.218748883144112
20	21.567529574343887	25.69304630633421	32.64435734623128	20.095066773090622
21	23.37475131342252	25.309443537722924	31.320808663227744	19.99499648562681
22	22.962557034107288	26.572235260480575	30.63342109338702	19.831786612025113
23	21.237535888302496	26.32205954182104	31.00630204548433	21.43410252439213
24	20.317842293992207	28.007767360407904	30.46782859389333	21.206561751706555
25	20.336903301128174	27.438319772220964	30.59768170500709	21.627095221643774
26	19.98308335616683	27.70040862034048	31.431600767205538	20.884907256287157
27	20.147484542714526	28.089967953681754	30.561942316627157	21.200605186976567
28	19.31713941935407	27.96130615551399	32.25122407405202	20.470330351079923
29	20.42863439797	27.823113853778246	31.6650981046211	20.083153643630645
30	19.997379111518804	27.746869825234388	32.60147008017536	19.65428098307144
31	19.857995496837063	28.47714466113103	31.237416757007896	20.427443085024006
32	19.49226242241574	28.24126469782347	32.07729238393633	20.18918049582445
33	18.798918287845034	28.008958673353902	32.62053108731133	20.571591951489737
34	19.86514337451305	27.75640032880237	31.202868681573964	21.175587615110615
35	20.208241502960416	27.87672293634815	32.27147639413398	19.643559166557463
36	20.346433804696158	27.98513241443395	30.480933036299305	21.187500744570592
37	18.802492226683025	27.46691128292491	31.46019227790948	22.27040421248258
38	19.486305857685757	26.008744237023624	32.43468626773567	22.070263637554948
39	18.636899727189334	26.65800979259242	32.51450423511753	22.190586245100725
40	18.611882155323382	26.312529038253057	34.17638579478443	20.899203011639127
41	17.89828570067071	26.41855589044686	33.46278934013176	22.22036906875067
42	17.82799823685684	26.59010495467054	33.870218367663	21.711678440809617
43	17.46703041421951	25.862212744665896	33.80707878152512	22.863678059589475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	15.0
2	19.0
3	75.5
4	132.0
5	132.0
6	187.0
7	242.0
8	265.5
9	289.0
10	460.5
11	632.0
12	632.0
13	1079.5
14	1527.0
15	2307.0
16	3087.0
17	2665.0
18	2243.0
19	2243.0
20	2432.0
21	2621.0
22	1831.5
23	1042.0
24	981.5
25	921.0
26	921.0
27	1038.5
28	1156.0
29	1416.5
30	1677.0
31	2111.5
32	2546.0
33	2546.0
34	3211.0
35	3876.0
36	4174.0
37	4472.0
38	4373.5
39	4275.0
40	4275.0
41	4800.5
42	5326.0
43	5625.0
44	5924.0
45	6052.5
46	6181.0
47	6181.0
48	6518.0
49	6855.0
50	6731.0
51	6607.0
52	5891.5
53	5176.0
54	5176.0
55	4832.0
56	4488.0
57	4006.5
58	3525.0
59	2965.0
60	2405.0
61	2405.0
62	2133.5
63	1862.0
64	1636.0
65	1410.0
66	1244.5
67	1079.0
68	1079.0
69	950.5
70	822.0
71	696.5
72	571.0
73	498.5
74	426.0
75	426.0
76	324.0
77	222.0
78	178.0
79	134.0
80	105.0
81	76.0
82	76.0
83	60.0
84	44.0
85	34.5
86	25.0
87	18.5
88	12.0
89	12.0
90	7.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	83941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.43130293896904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.86643163250227	46.762607069251025
2	8.727226667229623	9.84977543750968
3	3.018851991808989	5.110732538330494
4	1.5284257636851106	3.4500422916095825
5	0.8803225738352087	2.4838874924053798
6	0.49399396229601633	1.6726033761808887
7	0.44543900018999766	1.759569221238727
8	0.3082184551077709	1.391453520925412
9	0.23855263991217884	1.2115652660797465
>10	1.367983280204353	14.503043804577024
>50	0.08233232704933606	3.003299936860414
>100	0.03377736494331736	3.3487806911997717
>500	0.002111085308957335	0.6039956636208765
>1k	0.006333255926872005	4.848643690210982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	1.9406487890303903	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1403	1.6714120632348912	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1038	1.2365828379457	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.6039956636208765	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	409	0.4872469949130937	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	389	0.46342073599313804	RNA PCR Primer, Index 46 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	192	0.22873208563157454	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.2251581467935812	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	167	0.19894926198162996	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	165	0.19656663608963437	No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA	165	0.19656663608963437	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	158	0.18822744546764988	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.16320987360169645	No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT	137	0.16320987360169645	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.14653149235772744	No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA	123	0.14653149235772744	No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGG	122	0.14534017941172966	No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACA	117	0.13938361468174074	No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT	116	0.13819230173574296	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	102	0.12151392049177398	No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT	98	0.11674866870778286	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.11436604281578727	No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTAC	90	0.10721816513980058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011913129459977842	0.0
2	0.0	0.0	0.0	0.0011913129459977842	0.0
3	0.0	0.0	0.0	0.0011913129459977842	0.0
4	0.0	0.0	0.0	0.0011913129459977842	0.0
5	0.0	0.0	0.0	0.0011913129459977842	0.0
6	0.0	0.0	0.0	0.0011913129459977842	0.0
7	0.0	0.0	0.0	0.0011913129459977842	0.0
8	0.0	0.0	0.0	0.0011913129459977842	0.0
9	0.0	0.0	0.0	0.0011913129459977842	0.0
10	0.0	0.0	0.0	0.0011913129459977842	0.0
11	0.0	0.0	0.0	0.0011913129459977842	0.0
12	0.0	0.0	0.0	0.0011913129459977842	0.0
13	0.0	0.0	0.0	0.0011913129459977842	0.0
14	0.0	0.0	0.0	0.0035739388379933527	0.0
15	0.0	0.0	0.0	0.0035739388379933527	0.0
16	0.0	0.0	0.0	0.0035739388379933527	0.0
17	0.0	0.0	0.0	0.0035739388379933527	0.0
18	0.0	0.0	0.0	0.0035739388379933527	0.0
19	0.0	0.0	0.0	0.0035739388379933527	0.0
20	0.0	0.0	0.0	0.0035739388379933527	0.0
21	0.0	0.0	0.0	0.004765251783991137	0.0
22	0.0	0.0	0.0	0.010721816513980057	0.0
23	0.0	0.0	0.0	0.010721816513980057	0.0
24	0.0	0.0	0.0	0.013104442405975626	0.0
25	0.0	0.0	0.0	0.017869694189966763	0.0
26	0.0	0.0	0.0	0.023826258919955684	0.0
27	0.0	0.0	0.0	0.04884383078590915	0.0
28	0.0	0.0	0.0	0.12508785932976735	0.0
29	0.0	0.0	0.0	0.2620888481195125	0.0
30	0.0	0.0	0.0	0.4729512395611203	0.0
31	0.0	0.0	0.0	0.9566242956362206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTGA	55	1.8128048E-8	30.272728	13
TATGCCG	80	1.8189894E-12	30.0625	33
CCGTCTT	80	1.8189894E-12	30.0625	37
GCCGTCT	80	1.8189894E-12	30.0625	36
GTGTAGA	25	0.0054677106	29.599998	9
CCTGGAT	25	0.0054677106	29.599998	3
ATGCCGT	90	0.0	28.777779	34
GCCTTAT	40	5.8757465E-5	27.75	26
TTCCTCG	40	5.8757465E-5	27.75	20
GTATGCC	95	0.0	27.263159	32
GGCAGTC	55	6.146256E-7	26.90909	8
CTTATTG	55	6.146256E-7	26.90909	28
CAGTCGG	55	6.146256E-7	26.90909	10
CTACTAG	35	8.8007643E-4	26.42857	1
CGGTGAT	65	9.1766196E-8	25.615385	14
CGTATGC	95	9.094947E-12	25.31579	31
TGCCGTC	95	9.094947E-12	25.31579	35
GCAGTCG	60	1.312892E-6	24.666668	9
TCCTCGC	45	1.3089854E-4	24.666668	21
TTATTGA	60	1.312892E-6	24.666668	29
>>END_MODULE