FastQCFastQC Report
Fri 10 Feb 2017
ERR1632040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73665
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG810.10995723885155771No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT790.10724224529966742No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG790.10724224529966742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCTC250.005463419529.629
ATCAACG405.866128E-527.753
TATCAAC451.3068596E-424.6666682
CAACGCA451.3068596E-424.6666685
GTATCAA451.3068596E-424.6666681
TCAACGC451.3068596E-424.6666684
TGTGGAG400.001914170823.1253
CTTATAC400.001914170823.12537
ACGCAGA502.6689793E-422.27
AACGCAG502.6689793E-422.26
AGTACAT502.6689793E-422.213
GAGTACA555.08056E-420.18181812
CCAGGGC609.125419E-418.53
AGAGTAC609.125419E-418.511
GCAGAGT609.125419E-418.59
TCTCTTA1850.017.00000237
CGCAGAG700.002561738215.8571428
CAGAGTA700.002561738215.85714210
CTCCAGG750.004056114214.81
TACATGG750.004056114214.815
Produced by FastQC (version 0.11.5)