FastQCFastQC Report
Fri 10 Feb 2017
ERR1632039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306237
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.278509948807146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31430.2406148348270643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24320.18618367111021966No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA20240.15494891049633414No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG19370.14828855713013794No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT17860.13672863347156755No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG17850.13665207768574922No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC17410.13328362310974196No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC15040.11513990187079373No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC14210.10878577164787095No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG14170.10847954850459755No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC13650.10449864764204352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.103503422426405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC551.9029163E-523.5454543
GGTATCA18750.020.721
TAAGACT902.152674E-618.54
CTTATAC10600.017.10377337
CTAGCAC3000.016.653
CTAGATT3250.016.5076924
TCTAGCA3050.016.3770482
TTTGCGT700.00259274715.8571433
TCTTATA16800.015.85714237
ATCTCGC6700.015.46268611
TCGCCAT6000.015.41666713
CGAAAAC1201.935894E-615.41666623
GTATCAA25300.015.355732
GACATCG855.364845E-415.2352947
GTATGTA750.004104804714.81
TAGATTC3300.014.5757575
GCTAGAT3450.014.4782613
ATCTAGC2700.014.3888891
TCTCGCC6850.014.31386912
TATACAC3450.013.94202937
Produced by FastQC (version 0.11.5)