##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306237
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79877924143934	34.0	31.0	34.0	31.0	34.0
2	32.95575305247057	34.0	33.0	34.0	31.0	34.0
3	32.94803010479721	34.0	33.0	34.0	31.0	34.0
4	36.390823411065526	37.0	37.0	37.0	35.0	37.0
5	36.31388331520237	37.0	37.0	37.0	35.0	37.0
6	36.356400101972305	37.0	37.0	37.0	35.0	37.0
7	36.34679081973639	37.0	37.0	37.0	35.0	37.0
8	36.33308656851705	37.0	37.0	37.0	35.0	37.0
9	38.109480132625244	39.0	39.0	39.0	37.0	39.0
10	38.0646988257108	39.0	38.0	39.0	37.0	39.0
11	38.162462860874406	39.0	39.0	39.0	37.0	39.0
12	38.145332738239695	39.0	38.0	39.0	37.0	39.0
13	38.156898786361126	39.0	39.0	39.0	37.0	39.0
14	39.57614429846957	41.0	39.0	41.0	37.0	41.0
15	39.60510688336037	41.0	39.0	41.0	37.0	41.0
16	39.47647478979695	41.0	39.0	41.0	37.0	41.0
17	39.538079230644975	41.0	39.0	41.0	37.0	41.0
18	39.55077524216509	41.0	39.0	41.0	37.0	41.0
19	39.622378634198846	41.0	39.0	41.0	37.0	41.0
20	39.605606792641765	41.0	39.0	41.0	37.0	41.0
21	39.5736868577448	41.0	39.0	41.0	37.0	41.0
22	39.55925991990733	41.0	39.0	41.0	37.0	41.0
23	39.51941110227317	41.0	39.0	41.0	37.0	41.0
24	39.47025386664135	41.0	39.0	41.0	37.0	41.0
25	39.41600107790546	41.0	39.0	41.0	37.0	41.0
26	39.3281992471504	40.0	39.0	41.0	36.0	41.0
27	39.26239572145024	40.0	39.0	41.0	36.0	41.0
28	39.20274651537202	40.0	39.0	41.0	36.0	41.0
29	39.06832986663217	40.0	39.0	41.0	36.0	41.0
30	39.02178394885461	40.0	39.0	41.0	35.0	41.0
31	38.95125004114873	40.0	39.0	41.0	35.0	41.0
32	38.88086082387806	40.0	38.0	41.0	35.0	41.0
33	38.74766217769057	40.0	38.0	41.0	35.0	41.0
34	38.752910842366276	40.0	38.0	41.0	35.0	41.0
35	38.722227283410284	40.0	38.0	41.0	35.0	41.0
36	38.693960590612576	40.0	38.0	41.0	35.0	41.0
37	38.66233309881744	40.0	38.0	41.0	35.0	41.0
38	38.57500744505017	40.0	38.0	41.0	35.0	41.0
39	38.39102092499294	40.0	38.0	41.0	35.0	41.0
40	38.395243742138675	40.0	38.0	41.0	34.0	41.0
41	38.388442526126575	40.0	38.0	41.0	35.0	41.0
42	38.25264098322127	40.0	38.0	41.0	34.0	41.0
43	37.43913470526405	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	1.0
13	2.0
14	1.0
15	1.0
16	5.0
17	6.0
18	21.0
19	33.0
20	58.0
21	130.0
22	245.0
23	473.0
24	760.0
25	1080.0
26	1818.0
27	2601.0
28	3704.0
29	5298.0
30	7456.0
31	10431.0
32	14156.0
33	19627.0
34	29199.0
35	43899.0
36	71329.0
37	134935.0
38	310625.0
39	648336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55751444799068	18.384489185346915	13.052302147313236	26.005694219349166
2	18.99900247811079	20.85762384620861	34.77523604062662	25.368137635053976
3	20.541678118136293	22.138019364020465	30.000528234922147	27.319774282921095
4	15.649533736986474	15.840999757318158	32.671865825267545	35.83760068042782
5	15.558585463434277	35.643455207592496	33.21112478057198	15.586834548401246
6	34.9424338768539	33.21732579922326	15.806243430556629	16.03399689336621
7	29.09716996226565	29.839454861560345	20.80640802549614	20.256967150677863
8	26.667901766677872	33.35451376740974	20.09130043016696	19.886284035745426
9	26.284816614442864	14.808951208701023	19.681267641323892	39.22496453553222
10	17.584175000401917	27.01891004465499	32.30814928684458	23.088765668098514
11	35.42006542457456	21.7446757364858	21.16981834077583	21.66544049816381
12	21.656330359651427	25.63378621184364	28.045905911408113	24.66397751709682
13	30.403747558827384	19.518280373316635	24.65149892400843	25.426473143847556
14	23.33956242243942	21.122966199855004	24.06064136906243	31.476830008643148
15	26.067551294290393	27.37267433092157	22.0864207643789	24.473353610409138
16	25.63202542876982	25.929827435603187	23.491984992003747	24.946162143623248
17	24.338309204225574	26.4489522192374	24.609622909165797	24.603115667371238
18	23.234221661153374	25.065818836857325	26.550847970161616	25.149111531827685
19	24.99699518540663	25.393094821230754	26.28963962894942	23.320270364413194
20	25.07707253737262	24.54301937550383	26.581929619203866	23.79797846791968
21	24.940267348115235	25.413917994973346	25.063522163282776	24.582292493628643
22	25.159446562913164	24.696973060784526	24.986047708034604	25.157532668267702
23	24.17999183915323	25.475239179413844	25.402051848171503	24.94271713326142
24	25.488636441932055	25.33935265958628	25.166642806780086	24.00536809170158
25	25.09046979989083	24.5528950718744	25.49261734279461	24.86401778544016
26	25.005722544989922	24.91791305865628	25.314089250266225	24.76227514608758
27	24.926793529811206	24.687403587557235	25.105551289697047	25.280251592934516
28	23.964870081003678	25.059158483491128	26.105676075627933	24.870295359877264
29	24.29360062530766	25.502186816021897	25.723815815966017	24.480396742704425
30	23.717135558095507	26.448110105593397	25.613422372815958	24.22133196349514
31	24.78034231154071	25.0974363764003	25.7496151157868	24.37260619627219
32	23.55766985623589	25.25468196047119	25.920640741304986	25.26700744198794
33	23.979721903452436	24.87152025245036	26.06900585422094	25.079751989876264
34	24.29115084016147	24.751480780287192	25.869271809020873	25.08809657053046
35	24.39871171923625	24.777126968536336	25.929444656674093	24.894716655553317
36	23.693479820277638	24.937205116682502	26.43731573979301	24.931999323246853
37	24.50696160038339	23.47200393190516	26.397659842739106	25.623374624972346
38	23.37003162519512	24.072507515864274	27.80850641958542	24.748954439355188
39	23.292480614161136	23.427831243487972	28.33635856280292	24.943329579547967
40	22.962448621498243	23.014200332711447	29.040059346045165	24.98329169974515
41	22.033826939521695	23.679240444115422	29.466168849910083	24.820763766452796
42	22.618483475816408	22.99215226639576	28.918412202379812	25.470952055408013
43	21.41671075004	23.463735907036778	29.469843527629365	25.649709815293853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	96.0
2	109.0
3	269.0
4	429.0
5	429.0
6	577.5
7	726.0
8	779.0
9	832.0
10	1266.5
11	1701.0
12	1701.0
13	2995.5
14	4290.0
15	6592.0
16	8894.0
17	8435.0
18	7976.0
19	7976.0
20	8838.5
21	9701.0
22	10068.0
23	10435.0
24	11507.5
25	12580.0
26	12580.0
27	14347.0
28	16114.0
29	22536.5
30	28959.0
31	30944.5
32	32930.0
33	32930.0
34	36599.5
35	40269.0
36	43580.5
37	46892.0
38	56773.5
39	66655.0
40	66655.0
41	73783.5
42	80912.0
43	78227.0
44	75542.0
45	82215.5
46	88889.0
47	88889.0
48	94752.0
49	100615.0
50	106025.5
51	111436.0
52	116083.0
53	120730.0
54	120730.0
55	105234.5
56	89739.0
57	83259.0
58	76779.0
59	70781.5
60	64784.0
61	64784.0
62	60081.0
63	55378.0
64	49754.0
65	44130.0
66	38889.0
67	33648.0
68	33648.0
69	29250.5
70	24853.0
71	21813.5
72	18774.0
73	15397.0
74	12020.0
75	12020.0
76	9667.0
77	7314.0
78	6096.5
79	4879.0
80	3874.0
81	2869.0
82	2869.0
83	2232.5
84	1596.0
85	1250.0
86	904.0
87	719.0
88	534.0
89	534.0
90	398.0
91	262.0
92	156.5
93	51.0
94	33.5
95	16.0
96	16.0
97	11.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1306237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.91699050966245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.75840884069763	48.19427705447669
2	7.037463497018999	7.729526321557765
3	1.834300848714706	3.0220284690219383
4	0.8485673578546327	1.8640306215244884
5	0.4780485191871799	1.3126492995680272
6	0.3181051645651997	1.0481626982101009
7	0.23436997502203658	0.9009625585824506
8	0.17324487339445682	0.7611269654440845
9	0.1329476711375015	0.6570977394725905
>10	0.9391155943314284	10.09873876471355
>50	0.11895856774179865	4.58622888258896
>100	0.11271330096754803	12.886346472223225
>500	0.009825563833561582	3.6746876797387853
>1k	0.0039302255334246325	3.2641364728774076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3638	0.278509948807146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3143	0.2406148348270643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2432	0.18618367111021966	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2024	0.15494891049633414	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1937	0.14828855713013794	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1786	0.13672863347156755	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1785	0.13665207768574922	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1741	0.13328362310974196	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1504	0.11513990187079373	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1421	0.10878577164787095	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1417	0.10847954850459755	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1365	0.10449864764204352	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1352	0.103503422426405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.65557858183469E-5	0.0	0.0
2	0.0	0.0	7.65557858183469E-5	0.0	0.0
3	0.0	0.0	7.65557858183469E-5	0.0	0.0
4	0.0	0.0	7.65557858183469E-5	0.0	0.0
5	0.0	0.0	7.65557858183469E-5	0.0	0.0
6	0.0	0.0	7.65557858183469E-5	0.0	0.0
7	0.0	0.0	7.65557858183469E-5	0.0	0.0
8	0.0	0.0	7.65557858183469E-5	0.0	0.0
9	0.0	0.0	7.65557858183469E-5	0.0	0.0
10	0.0	0.0	7.65557858183469E-5	0.0	0.0
11	0.0	0.0	7.65557858183469E-5	7.65557858183469E-5	0.0
12	0.0	0.0	7.65557858183469E-5	7.65557858183469E-5	0.0
13	0.0	0.0	7.65557858183469E-5	7.65557858183469E-5	0.0
14	0.0	0.0	7.65557858183469E-5	1.531115716366938E-4	0.0
15	0.0	0.0	7.65557858183469E-5	3.062231432733876E-4	0.0
16	0.0	0.0	7.65557858183469E-5	9.186694298201629E-4	0.0
17	0.0	0.0	7.65557858183469E-5	0.001531115716366938	0.0
18	0.0	0.0	7.65557858183469E-5	0.002067006217095366	0.0
19	0.0	0.0	7.65557858183469E-5	0.0027560082894604885	0.0
20	0.0	0.0	7.65557858183469E-5	0.0032918987901889166	0.0
21	0.0	0.0	7.65557858183469E-5	0.004823014506555855	0.0
22	0.0	0.0	7.65557858183469E-5	0.00666035336619618	0.0
23	0.0	0.0	7.65557858183469E-5	0.008574248011654852	0.0
24	0.0	0.0	7.65557858183469E-5	0.012555148874208891	0.0
25	0.0	0.0	7.65557858183469E-5	0.014928378234577646	0.0
26	0.0	0.0	7.65557858183469E-5	0.019674836955315154	0.0
27	0.0	0.0	1.531115716366938E-4	0.037971669765900065	0.0
28	0.0	0.0	1.531115716366938E-4	0.08934060205001083	0.0
29	0.0	0.0	1.531115716366938E-4	0.16589638786835773	0.0
30	0.0	0.0	1.531115716366938E-4	0.27858650459296436	0.0
31	0.0	0.0	1.531115716366938E-4	0.6146664043355072	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	55	1.9029163E-5	23.545454	3
GGTATCA	1875	0.0	20.72	1
TAAGACT	90	2.152674E-6	18.5	4
CTTATAC	1060	0.0	17.103773	37
CTAGCAC	300	0.0	16.65	3
CTAGATT	325	0.0	16.507692	4
TCTAGCA	305	0.0	16.377048	2
TTTGCGT	70	0.002592747	15.857143	3
TCTTATA	1680	0.0	15.857142	37
ATCTCGC	670	0.0	15.462686	11
TCGCCAT	600	0.0	15.416667	13
CGAAAAC	120	1.935894E-6	15.416666	23
GTATCAA	2530	0.0	15.35573	2
GACATCG	85	5.364845E-4	15.235294	7
GTATGTA	75	0.0041048047	14.8	1
TAGATTC	330	0.0	14.575757	5
GCTAGAT	345	0.0	14.478261	3
ATCTAGC	270	0.0	14.388889	1
TCTCGCC	685	0.0	14.313869	12
TATACAC	345	0.0	13.942029	37
>>END_MODULE