FastQCFastQC Report
Fri 10 Feb 2017
ERR1632038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT40.3189792663476874RNA PCR Primer, Index 26 (100% over 22bp)
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC30.23923444976076555No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC30.23923444976076555No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT20.1594896331738437No Hit
AATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGATCAG20.1594896331738437No Hit
GAACTTACATCACTGGTAAAGGTCCCTTCAGCATGTCTCTCAA20.1594896331738437No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATC20.1594896331738437No Hit
TGGTGGTACTCTGTTCGTCAGGTGAGATATTCGGGCTGTCTCT20.1594896331738437No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA20.1594896331738437No Hit
GGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGTGATGT20.1594896331738437No Hit
TTTCTGAACACATGCACGGCCACATTGATGGCAGGACTGCCTC20.1594896331738437No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA20.1594896331738437No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG20.1594896331738437No Hit
GTCCGTGGAAGCCTGTGGCTACAAAAAATGTTGAGTCGTATGC20.1594896331738437No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGG20.1594896331738437No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA20.1594896331738437No Hit
GAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCT20.1594896331738437No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC20.1594896331738437No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)