FastQCFastQC Report
Fri 10 Feb 2017
ERR1632037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT441.3075780089153046TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT240.7132243684992571No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC210.6240713224368499No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC200.5943536404160475No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG170.5052005943536404No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC160.475482912332838RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.20802377414561665No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG60.17830609212481427No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)