##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3365
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20178306092125	33.0	31.0	34.0	30.0	34.0
2	32.386032689450225	34.0	31.0	34.0	31.0	34.0
3	32.47845468053492	34.0	31.0	34.0	30.0	34.0
4	35.96968796433878	37.0	35.0	37.0	35.0	37.0
5	35.832095096582464	37.0	35.0	37.0	35.0	37.0
6	35.812481426448734	37.0	35.0	37.0	35.0	37.0
7	35.78306092124814	37.0	35.0	37.0	35.0	37.0
8	35.78038632986627	37.0	35.0	37.0	35.0	37.0
9	37.49777117384844	39.0	37.0	39.0	35.0	39.0
10	37.43863298662704	39.0	37.0	39.0	34.0	39.0
11	37.525705794947996	39.0	37.0	39.0	35.0	39.0
12	37.533432392273404	39.0	37.0	39.0	35.0	39.0
13	37.56433878157504	39.0	37.0	39.0	35.0	39.0
14	38.755423476968794	40.0	38.0	41.0	35.0	41.0
15	38.7964338781575	40.0	38.0	41.0	36.0	41.0
16	38.69450222882615	40.0	38.0	41.0	35.0	41.0
17	38.7482912332838	40.0	38.0	41.0	35.0	41.0
18	38.69806835066865	40.0	38.0	41.0	35.0	41.0
19	38.857949479940565	40.0	38.0	41.0	35.0	41.0
20	38.77503714710252	40.0	38.0	41.0	35.0	41.0
21	38.75453194650817	40.0	38.0	41.0	35.0	41.0
22	38.685884101040116	40.0	38.0	41.0	35.0	41.0
23	38.734026745913816	40.0	38.0	41.0	35.0	41.0
24	38.59970282317979	40.0	38.0	41.0	34.0	41.0
25	38.463595839524515	40.0	38.0	41.0	34.0	41.0
26	38.49153046062407	40.0	38.0	41.0	34.0	41.0
27	38.270133729569096	40.0	38.0	41.0	34.0	41.0
28	38.265973254086184	40.0	38.0	41.0	34.0	41.0
29	38.237147102526	40.0	38.0	41.0	34.0	41.0
30	38.12689450222883	40.0	38.0	41.0	34.0	41.0
31	38.0258543833581	40.0	37.0	41.0	33.0	41.0
32	37.86448736998514	40.0	37.0	41.0	33.0	41.0
33	37.70995542347697	40.0	37.0	41.0	33.0	41.0
34	37.7260029717682	40.0	37.0	41.0	33.0	41.0
35	37.74918276374443	40.0	37.0	41.0	33.0	41.0
36	37.681723625557204	40.0	37.0	41.0	33.0	41.0
37	37.64279346210996	40.0	37.0	41.0	32.0	41.0
38	37.58127786032689	40.0	37.0	41.0	33.0	41.0
39	37.43476968796434	40.0	37.0	41.0	33.0	41.0
40	37.363150074294204	39.0	36.0	41.0	32.0	41.0
41	37.394947994056466	39.0	37.0	41.0	32.0	41.0
42	37.233580980683506	39.0	37.0	41.0	31.0	41.0
43	36.33135215453195	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	3.0
24	5.0
25	7.0
26	18.0
27	14.0
28	17.0
29	34.0
30	35.0
31	54.0
32	52.0
33	83.0
34	136.0
35	186.0
36	289.0
37	506.0
38	947.0
39	977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38632986627043	19.554234769687966	13.699851411589897	26.35958395245171
2	20.74294205052006	19.13818722139673	32.5111441307578	27.60772659732541
3	19.673105497771175	22.199108469539375	31.054977711738484	27.072808320950966
4	15.334323922734027	15.63150074294205	32.065378900445765	36.96879643387816
5	14.829123328380387	35.0668647845468	33.937592867756315	16.166419019316493
6	35.037147102526	33.7592867756315	15.0074294205052	16.196136701337295
7	29.06389301634473	28.826151560178303	19.702823179791977	22.407132243684995
8	28.855869242199105	29.71768202080238	21.2481426448737	20.178306092124814
9	25.349182763744427	13.580980683506686	18.98959881129272	42.080237741456166
10	16.612184249628527	24.665676077265974	32.80832095096582	25.913818722139677
11	34.94799405646359	20.0	20.50520059435364	24.546805349182762
12	22.436849925705797	27.25111441307578	25.468053491827636	24.843982169390788
13	32.451708766716195	17.028231797919762	23.447251114413074	27.072808320950966
14	25.0222882615156	19.940564635958395	22.10995542347697	32.92719167904903
15	27.072808320950966	26.508172362555722	20.326894502228825	26.09212481426449
16	27.369985141158992	25.052005943536404	19.554234769687966	28.023774145616642
17	22.4962852897474	27.01337295690936	23.38781575037147	27.102526002971768
18	25.616641901931647	22.85289747399703	23.447251114413074	28.083209509658246
19	26.537890044576525	24.219910846953937	23.566121842496283	25.67607726597325
20	26.686478454680536	23.298662704309063	23.566121842496283	26.448736998514118
21	28.707280832095094	23.625557206537888	21.931649331352155	25.735512630014856
22	26.508172362555722	24.01188707280832	23.23922734026746	26.240713224368502
23	26.775631500742943	24.01188707280832	21.783060921248143	27.429420505200596
24	26.686478454680536	23.298662704309063	24.457652303120355	25.557206537890043
25	24.933135215453195	24.071322436849925	24.9925705794948	26.002971768202084
26	26.151560178306095	24.071322436849925	23.38781575037147	26.389301634472513
27	24.665676077265974	25.319465081723624	24.933135215453195	25.081723625557206
28	25.052005943536404	22.377414561664192	25.200594353640415	27.369985141158992
29	25.052005943536404	24.160475482912332	23.15007429420505	27.637444279346212
30	24.903417533432393	22.4665676077266	27.429420505200596	25.200594353640415
31	25.854383358098072	24.13075780089153	23.566121842496283	26.448736998514118
32	26.389301634472513	24.041604754829123	25.557206537890043	24.01188707280832
33	24.190193164933135	25.11144130757801	25.37890044576523	25.319465081723624
34	23.684992570579492	24.9925705794948	26.2109955423477	25.11144130757801
35	24.457652303120355	23.15007429420505	27.667161961367015	24.72511144130758
36	25.913818722139677	24.72511144130758	23.358098068350667	26.002971768202084
37	24.27934621099554	23.50668647845468	24.635958395245172	27.578008915304608
38	24.51708766716196	23.38781575037147	27.25111441307578	24.843982169390788
39	25.468053491827636	22.88261515601783	26.06240713224369	25.586924219910845
40	22.58543833580981	22.942050520059436	29.59881129271917	24.87369985141159
41	23.744427934621097	22.10995542347697	26.894502228826152	27.25111441307578
42	22.555720653789006	22.644873699851413	28.736998514115896	26.06240713224369
43	22.704309063893017	20.713224368499258	29.301634472511147	27.28083209509658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.0
4	4.0
5	4.0
6	2.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.0
15	7.0
16	12.0
17	11.5
18	11.0
19	11.0
20	12.0
21	13.0
22	11.5
23	10.0
24	14.0
25	18.0
26	18.0
27	20.5
28	23.0
29	27.0
30	31.0
31	43.5
32	56.0
33	56.0
34	72.5
35	89.0
36	93.0
37	97.0
38	118.5
39	140.0
40	140.0
41	150.0
42	160.0
43	181.5
44	203.0
45	228.0
46	253.0
47	253.0
48	248.0
49	243.0
50	272.5
51	302.0
52	297.5
53	293.0
54	293.0
55	306.0
56	319.0
57	313.0
58	307.0
59	252.5
60	198.0
61	198.0
62	195.0
63	192.0
64	160.5
65	129.0
66	114.5
67	100.0
68	100.0
69	81.0
70	62.0
71	49.5
72	37.0
73	32.5
74	28.0
75	28.0
76	20.0
77	12.0
78	10.5
79	9.0
80	7.5
81	6.0
82	6.0
83	4.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3365.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.8484398216939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.00506649778342	91.97622585438336
2	1.5832805573147564	2.9717682020802374
3	0.15832805573147563	0.44576523031203563
4	0.0	0.0
5	0.0	0.0
6	0.031665611146295125	0.17830609212481427
7	0.031665611146295125	0.20802377414561665
8	0.0	0.0
9	0.0	0.0
>10	0.18999366687777072	4.219910846953938
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	44	1.3075780089153046	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	24	0.7132243684992571	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	21	0.6240713224368499	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	20	0.5943536404160475	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	17	0.5052005943536404	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	16	0.475482912332838	RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.20802377414561665	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	6	0.17830609212481427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.029717682020802376	0.0
27	0.0	0.0	0.0	0.029717682020802376	0.0
28	0.0	0.0	0.0	0.05943536404160475	0.0
29	0.0	0.0	0.0	0.17830609212481427	0.0
30	0.0	0.0	0.0	0.2674591381872214	0.0
31	0.0	0.0	0.0	0.5052005943536404	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE