FastQCFastQC Report
Fri 10 Feb 2017
ERR1632036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237443
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2560.10781534936805887No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG2470.10402496599183804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2420.10191919744949314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATT250.005487670729.635
GCCGTCT400.001926701223.12536
GGTATCA2050.021.6585351
GTGGGTA450.003817006520.5555559
TGAACCA450.003817006520.5555555
ATACTTT450.003817006520.5555556
TAGGACA759.215093E-619.7333324
CTCTTAT3300.019.62121237
TCGGGAG701.214034E-418.53
CCGTCTT500.00701885118.537
CTCTATC500.00701885118.59
CTCTAGA500.00701885118.51
AATACTT500.00701885118.55
TGCCGTC500.00701885118.535
GTCTTGA1054.7577487E-717.6190471
AATTTAA752.0585749E-417.26666623
CGTTGCC752.0585749E-417.26666614
GAACGTT752.0585749E-417.26666611
GCTTAGG803.3684453E-416.18751
GTATCAA2750.016.1454542