FastQCFastQC Report
Fri 10 Feb 2017
ERR1632035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224026
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.12989563711354932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2750.12275360895610331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2340.10445216180264791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG200.001838774337.05
AGTTTAG250.005487017329.61
TTAGAAC250.005487017329.63
TAGAACT250.005487017329.64
ACGGGGT250.005487017329.612
GGTATCA2000.024.0500011
AGGACAG652.6668658E-622.769235
TGTAGAA851.2361743E-619.5882342
GTAGAAC1002.8493378E-718.53
AGACAGT500.007017643718.56
GGACAGT500.007017643718.56
CTGTAGA953.580235E-617.5263161
CAGATGT650.001574571117.0769234
TAGTAGA650.001574571117.0769234
GTCTTGC700.002583767515.8571421
GAACAGA1201.9185318E-615.4166676
TCTTATA2055.456968E-1215.34146337
TAGAACA1101.4414412E-515.1363644
TAATCCC1101.4414412E-515.1363645
CTTATAC1353.9304905E-715.07407337
Produced by FastQC (version 0.11.5)