##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224026
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68529545677734	34.0	31.0	34.0	31.0	34.0
2	32.84681242355798	34.0	31.0	34.0	31.0	34.0
3	32.83918384473231	34.0	31.0	34.0	31.0	34.0
4	36.32077080338889	37.0	37.0	37.0	35.0	37.0
5	36.24134698651049	37.0	37.0	37.0	35.0	37.0
6	36.27694106934017	37.0	37.0	37.0	35.0	37.0
7	36.26652709953309	37.0	37.0	37.0	35.0	37.0
8	36.25016739128494	37.0	37.0	37.0	35.0	37.0
9	38.020680635283405	39.0	38.0	39.0	35.0	39.0
10	37.949505860926855	39.0	38.0	39.0	35.0	39.0
11	38.056087239873946	39.0	38.0	39.0	35.0	39.0
12	38.02496585217787	39.0	38.0	39.0	35.0	39.0
13	38.048766661012564	39.0	38.0	39.0	35.0	39.0
14	39.41712569076804	40.0	39.0	41.0	37.0	41.0
15	39.44914429575139	40.0	39.0	41.0	37.0	41.0
16	39.32870291841125	40.0	39.0	41.0	37.0	41.0
17	39.38933427370037	40.0	39.0	41.0	37.0	41.0
18	39.413746618696045	40.0	39.0	41.0	37.0	41.0
19	39.47740440841688	40.0	39.0	41.0	37.0	41.0
20	39.46723148206012	40.0	39.0	41.0	37.0	41.0
21	39.43496736985885	40.0	39.0	41.0	37.0	41.0
22	39.417241748725594	40.0	39.0	41.0	37.0	41.0
23	39.38656227402176	40.0	39.0	41.0	37.0	41.0
24	39.334742395971894	40.0	39.0	41.0	36.0	41.0
25	39.277088373670914	40.0	39.0	41.0	36.0	41.0
26	39.19212948497049	40.0	39.0	41.0	36.0	41.0
27	39.12229384089347	40.0	39.0	41.0	36.0	41.0
28	39.077053556283644	40.0	39.0	41.0	36.0	41.0
29	38.933913920705635	40.0	39.0	41.0	35.0	41.0
30	38.89952951889513	40.0	38.0	41.0	35.0	41.0
31	38.838433931775775	40.0	38.0	41.0	35.0	41.0
32	38.78427950327194	40.0	38.0	41.0	35.0	41.0
33	38.64026943301224	40.0	38.0	41.0	35.0	41.0
34	38.654553489327135	40.0	38.0	41.0	35.0	41.0
35	38.62900288359387	40.0	38.0	41.0	35.0	41.0
36	38.619088855757816	40.0	38.0	41.0	35.0	41.0
37	38.607255407854446	40.0	38.0	41.0	35.0	41.0
38	38.5357815610688	40.0	38.0	41.0	35.0	41.0
39	38.35479810379152	40.0	38.0	41.0	34.0	41.0
40	38.381268245650055	40.0	38.0	41.0	34.0	41.0
41	38.393467722496496	40.0	38.0	41.0	34.0	41.0
42	38.27170060617964	40.0	38.0	41.0	34.0	41.0
43	37.45896458446787	39.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	2.0
19	8.0
20	12.0
21	27.0
22	60.0
23	98.0
24	117.0
25	196.0
26	314.0
27	477.0
28	693.0
29	982.0
30	1397.0
31	1952.0
32	2742.0
33	3775.0
34	5308.0
35	8085.0
36	12974.0
37	23909.0
38	57831.0
39	103064.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.28169944560006	18.650067402890734	12.92707096497728	26.14116218653192
2	19.01073982484176	20.87704105773437	34.11612937784007	25.996089739583798
3	19.999017971128353	21.942542383473345	30.544668922357225	27.513770723041077
4	15.383035897619028	15.733441654093722	33.96882504709275	34.9146974011945
5	15.478560524224866	35.39812343210163	33.81705694874702	15.30625909492648
6	35.04771767562694	34.18710328265469	15.574085150830708	15.191093890887664
7	29.35552123414247	30.209439975717107	20.790890343085177	19.64414844705525
8	27.49814753644666	32.94662226705829	20.053475935828878	19.501754260666175
9	26.74689544963531	14.082740396204013	19.444618035406606	39.725746118754074
10	16.962763250694117	26.83215341076482	33.226946872238045	22.978136466303017
11	36.328818976368815	21.068983064465733	20.965870032942604	21.636327926222847
12	22.369278565880744	25.419817342629873	28.223956147947117	23.986947943542265
13	31.775776025996983	19.053591993786437	23.51602046191067	25.65461151830591
14	23.591458134323695	20.524403417460473	24.28602037263532	31.598118075580512
15	26.230883915259835	27.14863453349165	21.677394588128163	24.943086963120354
16	25.853695553194715	25.38321444832296	23.082142251345825	25.680947747136496
17	24.272629069840107	26.131788274575275	24.469034844169872	25.126547811414746
18	23.96284359851089	24.624373956594322	25.9474346727612	25.46534777213359
19	25.31759706462643	25.087713033308635	25.790756430057225	23.803933472007714
20	26.005017274780606	24.25522037620633	25.65104050422719	24.088721844785873
21	26.064831760599215	24.803371037290315	24.138716041887996	24.993081160222474
22	26.192941890673403	24.605626132681028	24.332443555658717	24.86898842098685
23	25.336791265299563	24.790872488014784	24.53509860462625	25.337237642059403
24	25.94609554248168	24.57348700597252	24.56143483345683	24.91898261808897
25	25.490791247444495	24.225759510056868	24.707400033924635	25.576049208574002
26	25.009150723576727	24.357440654209782	24.629730477712407	26.003678144501087
27	24.877469579423817	24.50876237579567	25.006472463017687	25.60729558176283
28	24.650710185424906	24.670350762857883	25.170739110638944	25.508199941078267
29	24.507869622275987	25.064055065037095	25.402855025755937	25.02522028693098
30	24.483765277244608	25.69969557104979	24.806942051369038	25.009597100336567
31	25.181898529634957	25.04932463196236	24.91675073428977	24.852026104112916
32	24.09809575674252	25.196182585949845	25.301081124512333	25.4046405327953
33	24.315481238784784	24.404310213993018	25.66130716970352	25.618901377518682
34	24.65829859034219	23.982037799184024	25.80995063073036	25.549712979743422
35	24.402078330193817	24.563666717256034	25.701034701329313	25.33322025122084
36	24.258791390285054	24.502513101157902	25.785846285698984	25.45284922285806
37	24.717220322641122	23.36112772624606	25.859052074312807	26.062599876800014
38	23.863747957826323	23.69367841232714	27.03346932945283	25.409104300393704
39	23.529858141465724	23.11026398721577	27.904350387901406	25.4555274834171
40	23.139724853365234	22.532206083222484	28.796211154062473	25.53185790934981
41	22.44426986153393	22.671475632292683	29.222500959710036	25.661753546463355
42	22.320177122298304	22.254113361841927	29.468900931141924	25.956808584717844
43	21.73051342254917	21.83942935195022	29.984019711997718	26.446037513502894
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	21.0
4	38.0
5	38.0
6	54.0
7	70.0
8	63.0
9	56.0
10	87.0
11	118.0
12	118.0
13	214.0
14	310.0
15	495.0
16	680.0
17	718.0
18	756.0
19	756.0
20	875.5
21	995.0
22	1125.5
23	1256.0
24	1616.0
25	1976.0
26	1976.0
27	2336.0
28	2696.0
29	3620.5
30	4545.0
31	5083.5
32	5622.0
33	5622.0
34	6491.5
35	7361.0
36	8113.5
37	8866.0
38	10108.0
39	11350.0
40	11350.0
41	12574.5
42	13799.0
43	13887.5
44	13976.0
45	14582.5
46	15189.0
47	15189.0
48	15759.5
49	16330.0
50	16939.0
51	17548.0
52	18448.0
53	19348.0
54	19348.0
55	17939.0
56	16530.0
57	15863.5
58	15197.0
59	14100.0
60	13003.0
61	13003.0
62	11690.5
63	10378.0
64	9303.5
65	8229.0
66	7058.0
67	5887.0
68	5887.0
69	4954.5
70	4022.0
71	3489.5
72	2957.0
73	2434.5
74	1912.0
75	1912.0
76	1574.0
77	1236.0
78	995.5
79	755.0
80	622.5
81	490.0
82	490.0
83	375.0
84	260.0
85	205.0
86	150.0
87	116.0
88	82.0
89	82.0
90	56.5
91	31.0
92	20.5
93	10.0
94	6.5
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	224026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.86007452144722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.79526055072009	74.10636422817618
2	4.489092784137364	7.169985685498018
3	0.9360195475157188	2.242517724544098
4	0.4587653139303222	1.4654812863332263
5	0.2892252453337662	1.1548774822919208
6	0.1970332106317955	0.9441052130553116
7	0.12390826619752884	0.6926726360650581
8	0.08484076044046908	0.5420311560985689
9	0.07127665818586015	0.5122943310926261
>10	0.5063576567226918	7.679274748174819
>50	0.03812744675042494	2.168567729119912
>100	0.010092559433936013	1.3218277795502749
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.12989563711354932	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.12275360895610331	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.10445216180264791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	8.927535196807514E-4	0.0
18	0.0	0.0	0.0	8.927535196807514E-4	0.0
19	0.0	0.0	0.0	0.001339130279521127	0.0
20	0.0	0.0	0.0	0.001339130279521127	0.0
21	0.0	0.0	0.0	0.0017855070393615028	0.0
22	0.0	0.0	0.0	0.0022318837992018783	0.0
23	0.0	0.0	0.0	0.002678260559042254	0.0
24	0.0	0.0	0.0	0.005356521118084508	0.0
25	0.0	0.0	0.0	0.006695651397605635	0.0
26	0.0	0.0	0.0	0.011605795755849767	0.0
27	0.0	0.0	0.0	0.024550721791220663	0.0
28	0.0	0.0	0.0	0.07499129565318312	0.0
29	0.0	0.0	0.0	0.1562318659441315	0.0
30	0.0	0.0	0.0	0.2562202601483756	0.0
31	0.0	0.0	0.0	0.5744868899145635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAG	20	0.0018387743	37.0	5
AGTTTAG	25	0.0054870173	29.6	1
TTAGAAC	25	0.0054870173	29.6	3
TAGAACT	25	0.0054870173	29.6	4
ACGGGGT	25	0.0054870173	29.6	12
GGTATCA	200	0.0	24.050001	1
AGGACAG	65	2.6668658E-6	22.76923	5
TGTAGAA	85	1.2361743E-6	19.588234	2
GTAGAAC	100	2.8493378E-7	18.5	3
AGACAGT	50	0.0070176437	18.5	6
GGACAGT	50	0.0070176437	18.5	6
CTGTAGA	95	3.580235E-6	17.526316	1
CAGATGT	65	0.0015745711	17.076923	4
TAGTAGA	65	0.0015745711	17.076923	4
GTCTTGC	70	0.0025837675	15.857142	1
GAACAGA	120	1.9185318E-6	15.416667	6
TCTTATA	205	5.456968E-12	15.341463	37
TAGAACA	110	1.4414412E-5	15.136364	4
TAATCCC	110	1.4414412E-5	15.136364	5
CTTATAC	135	3.9304905E-7	15.074073	37
>>END_MODULE