FastQCFastQC Report
Fri 10 Feb 2017
ERR1632034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822031
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.1305303571276509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9900.12043341431162571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.024.6666661
ATAATAC450.003824895220.5555553
GTGTTAT555.1413826E-420.1818181
ACTAGAA851.2444252E-619.5882362
GTATTGG902.1506985E-618.51
ATAAGGT500.00703321818.53
TATAGAG609.233764E-418.55
CTAGCAC1054.793146E-717.6190473
GTATTAT752.0663635E-417.2666661
CTTATAC6450.017.20930337
CGCCTAG650.001579416917.07692116
GACGTAG803.3810997E-416.18751
GTATGTA700.002591651415.8571421
TCTAAGC700.002591651415.8571423
CCGCCTA700.002591651415.85714215
GTATCAA7150.015.7832162
GTATTAA855.3618423E-415.2352941
CCTTAAG750.004103082714.81
GTATTAG1508.090137E-814.81
CGAAAGC1001.09276174E-414.819
Produced by FastQC (version 0.11.5)