##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632034.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	822031
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.588459559311026	34.0	31.0	34.0	31.0	34.0
2	32.75015297476616	34.0	31.0	34.0	31.0	34.0
3	32.74749735715563	34.0	31.0	34.0	31.0	34.0
4	36.262525379213194	37.0	37.0	37.0	35.0	37.0
5	36.146116144038366	37.0	35.0	37.0	35.0	37.0
6	36.17450193484187	37.0	36.0	37.0	35.0	37.0
7	36.16969433026249	37.0	36.0	37.0	35.0	37.0
8	36.14641540282544	37.0	36.0	37.0	35.0	37.0
9	37.88792636774039	39.0	38.0	39.0	35.0	39.0
10	37.80695253585327	39.0	38.0	39.0	35.0	39.0
11	37.91989109899748	39.0	38.0	39.0	35.0	39.0
12	37.88349344489441	39.0	38.0	39.0	35.0	39.0
13	37.90800833545207	39.0	38.0	39.0	35.0	39.0
14	39.26348154753288	40.0	39.0	41.0	36.0	41.0
15	39.28231782013087	40.0	39.0	41.0	36.0	41.0
16	39.157885773164274	40.0	39.0	41.0	36.0	41.0
17	39.205497116288804	40.0	39.0	41.0	36.0	41.0
18	39.21118303324327	40.0	39.0	41.0	36.0	41.0
19	39.26773929450349	40.0	39.0	41.0	36.0	41.0
20	39.24064299278251	40.0	39.0	41.0	36.0	41.0
21	39.21237276939677	40.0	39.0	41.0	36.0	41.0
22	39.18848315939423	40.0	39.0	41.0	36.0	41.0
23	39.142280522267406	40.0	39.0	41.0	36.0	41.0
24	39.084381245962746	40.0	39.0	41.0	36.0	41.0
25	39.02221205769612	40.0	39.0	41.0	36.0	41.0
26	38.923415296990015	40.0	38.0	41.0	35.0	41.0
27	38.85344956577063	40.0	38.0	41.0	35.0	41.0
28	38.77867379697359	40.0	38.0	41.0	35.0	41.0
29	38.64345261918346	40.0	38.0	41.0	35.0	41.0
30	38.58452418461104	40.0	38.0	41.0	35.0	41.0
31	38.51734666940784	40.0	38.0	41.0	35.0	41.0
32	38.44518759024903	40.0	38.0	41.0	34.0	41.0
33	38.29610562132085	40.0	38.0	41.0	34.0	41.0
34	38.29001825965201	40.0	38.0	41.0	34.0	41.0
35	38.25578475750915	40.0	38.0	41.0	34.0	41.0
36	38.22509491734497	40.0	38.0	41.0	34.0	41.0
37	38.1979110276863	40.0	38.0	41.0	34.0	41.0
38	38.12947808537634	40.0	38.0	41.0	34.0	41.0
39	37.94032706795729	40.0	37.0	41.0	33.0	41.0
40	37.938634917661254	40.0	37.0	41.0	33.0	41.0
41	37.9358016911771	40.0	37.0	41.0	33.0	41.0
42	37.80697078334029	40.0	37.0	41.0	33.0	41.0
43	36.92122073255145	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	0.0
16	2.0
17	7.0
18	23.0
19	31.0
20	75.0
21	141.0
22	260.0
23	472.0
24	700.0
25	1104.0
26	1650.0
27	2373.0
28	3363.0
29	4720.0
30	6605.0
31	8965.0
32	11921.0
33	16326.0
34	23602.0
35	34700.0
36	55107.0
37	98705.0
38	226499.0
39	324673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46968788281707	18.07048639284893	12.650739448998882	26.809086275335115
2	19.784655323217738	20.159580356458576	33.38633214562468	26.669432174699008
3	20.70846476592732	21.34067936610663	29.372006651817266	28.578849216148782
4	15.933462363341528	15.59515395404796	32.5245641587726	35.94681952383791
5	16.503148908009553	34.430331702819	32.790490869565744	16.27602851960571
6	35.89171211304683	32.86433236702752	15.290542570778962	15.953412949146687
7	30.369900891815515	29.035158041485055	20.220283663268173	20.374657403431257
8	27.646767579324866	32.22676517065658	19.594516508501506	20.531950741517047
9	26.90397320782306	14.284254486752932	19.05280944392608	39.75896286149793
10	18.2269281815406	25.876396388943967	31.655740476940654	24.24093495257478
11	37.09616790607653	20.754205133383046	20.406529680754133	21.743097279786287
12	22.69452125284813	24.43812946227089	27.461859710886817	25.405489573994167
13	31.594793870304162	18.62983269487404	23.55117994333547	26.224193491486332
14	23.981577337107723	20.084644009775786	23.374057669357967	32.559720983758524
15	27.083893429809823	26.22577494036113	20.87609834665603	25.81423328317302
16	26.687922961542814	24.775464672256888	22.641481890585634	25.895130475614664
17	25.55755196580178	25.21681055823929	23.680737101155554	25.54490037480338
18	24.90125068276014	23.882554307562607	25.12374837445303	26.09244663522422
19	26.008264895119527	24.254194793140403	24.910496076182042	24.827044235558027
20	26.165558233205317	23.61334304910642	24.874487701802	25.34661101588626
21	26.4086147602706	24.00748876867174	23.908952338780413	25.67494413227725
22	26.68500336362011	23.754578598617325	23.617600796077035	25.942817241685535
23	25.639057407810657	23.987416532953137	24.168918203814698	26.204607855421514
24	26.130158108392504	24.16612025580544	23.963816449744595	25.73990518605746
25	26.23623683291749	23.464321905134963	24.11721699060011	26.182224271347433
26	26.220787293909844	24.051161087598885	24.14142532337588	25.586626295115387
27	26.128698309431154	23.631347236296442	23.955422605716816	26.28453184855559
28	25.44247114768178	23.915643084019948	24.66500655084784	25.97687921745044
29	25.34296151848288	24.144709871038927	24.673400394875618	25.838928215602575
30	24.9475992997831	24.87485265154234	24.54688448489169	25.630663563782875
31	25.91301301289124	24.017342411660874	24.27645672730104	25.793187848146847
32	24.952100346580604	24.115027292158082	24.681429289163063	26.25144307209825
33	25.107203986224363	23.767716789269507	24.884462994704577	26.24061622980155
34	25.779806357667777	23.498262230986423	24.668047798683993	26.05388361266181
35	25.50548580284685	23.668572109810942	24.99917886308424	25.826763224257967
36	25.00574795841033	23.69630829007665	25.28128501236571	26.016658739147307
37	25.392716333082326	22.56545069468183	25.576772652126255	26.465060320109586
38	24.47632753509296	22.717999686143223	26.758844861081883	26.046827917681938
39	24.468176990892072	22.25487846565397	27.0769593847434	26.19998515871056
40	23.944230813679777	22.04040966824852	28.028261707891794	25.98709781017991
41	23.20301789105277	22.284682694448264	28.565102775929375	25.947196638569594
42	23.567116081996907	21.95270008065389	28.315355503624563	26.16482833372464
43	22.503044289083988	21.92107110315791	28.86034711586303	26.715537491895073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	27.0
2	25.0
3	78.0
4	131.0
5	131.0
6	171.0
7	211.0
8	227.5
9	244.0
10	405.0
11	566.0
12	566.0
13	890.0
14	1214.0
15	1923.0
16	2632.0
17	2561.5
18	2491.0
19	2491.0
20	2930.5
21	3370.0
22	3604.5
23	3839.0
24	4565.5
25	5292.0
26	5292.0
27	6476.0
28	7660.0
29	10457.5
30	13255.0
31	15078.5
32	16902.0
33	16902.0
34	19582.0
35	22262.0
36	24874.0
37	27486.0
38	32246.5
39	37007.0
40	37007.0
41	40757.0
42	44507.0
43	45770.0
44	47033.0
45	50654.5
46	54276.0
47	54276.0
48	57535.5
49	60795.0
50	64064.5
51	67334.0
52	70119.0
53	72904.0
54	72904.0
55	67350.5
56	61797.0
57	59054.0
58	56311.0
59	52776.0
60	49241.0
61	49241.0
62	46213.0
63	43185.0
64	38956.5
65	34728.0
66	30732.0
67	26736.0
68	26736.0
69	22964.0
70	19192.0
71	17035.5
72	14879.0
73	12103.0
74	9327.0
75	9327.0
76	7586.5
77	5846.0
78	4894.0
79	3942.0
80	3192.0
81	2442.0
82	2442.0
83	1932.0
84	1422.0
85	1096.5
86	771.0
87	611.0
88	451.0
89	451.0
90	341.0
91	231.0
92	139.0
93	47.0
94	28.5
95	10.0
96	10.0
97	7.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	822031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.6928360398042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.04565982736409	54.571392795335086
2	7.418180816664277	9.301335873055086
3	2.030672792522022	3.8192590929622328
4	0.9347769349407608	2.344152684641273
5	0.5340306333429742	1.6739947468201937
6	0.3450816768380871	1.2980489391810532
7	0.23569121136211543	1.0343305328963557
8	0.17878236574675105	0.8966698834055497
9	0.1435653754493753	0.8100468489636555
>10	0.9525325818392969	11.758630179318187
>50	0.1148978162954743	5.051312387881649
>100	0.0647624933795764	6.808935494480339
>500	0.00117040650446124	0.5006696100439029
>1k	1.9506775074354E-4	0.1312209310154147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1073	0.1305303571276509	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	990	0.12043341431162571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2164991344608658E-4	0.0
11	0.0	0.0	0.0	1.2164991344608658E-4	0.0
12	0.0	0.0	0.0	2.4329982689217316E-4	0.0
13	0.0	0.0	0.0	3.6494974033825976E-4	0.0
14	0.0	0.0	0.0	4.865996537843463E-4	0.0
15	0.0	0.0	0.0	4.865996537843463E-4	0.0
16	0.0	0.0	0.0	8.515493941226061E-4	0.0
17	0.0	0.0	0.0	9.731993075686926E-4	0.0
18	0.0	0.0	0.0	9.731993075686926E-4	0.0
19	0.0	0.0	0.0	0.0013381490479069523	0.0
20	0.0	0.0	0.0	0.0015814488747991256	0.0
21	0.0	0.0	0.0	0.002311348355475645	0.0
22	0.0	0.0	0.0	0.0030412478361521644	0.0
23	0.0	0.0	0.0	0.00462269671095129	0.0
24	0.0	1.2164991344608658E-4	0.0	0.007055694979873022	0.0
25	0.0	1.2164991344608658E-4	0.0	0.00888044368156432	0.0
26	0.0	1.2164991344608658E-4	0.0	0.011921691517716486	0.0
27	0.0	1.2164991344608658E-4	0.0	0.03418362567835033	0.0
28	0.0	1.2164991344608658E-4	0.0	0.09476528257450145	0.0
29	0.0	1.2164991344608658E-4	0.0	0.17298617692033513	0.0
30	0.0	1.2164991344608658E-4	0.0	0.2952443399336521	0.0
31	0.0	1.2164991344608658E-4	0.0	0.6188331097002424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	465	0.0	24.666666	1
ATAATAC	45	0.0038248952	20.555555	3
GTGTTAT	55	5.1413826E-4	20.181818	1
ACTAGAA	85	1.2444252E-6	19.588236	2
GTATTGG	90	2.1506985E-6	18.5	1
ATAAGGT	50	0.007033218	18.5	3
TATAGAG	60	9.233764E-4	18.5	5
CTAGCAC	105	4.793146E-7	17.619047	3
GTATTAT	75	2.0663635E-4	17.266666	1
CTTATAC	645	0.0	17.209303	37
CGCCTAG	65	0.0015794169	17.076921	16
GACGTAG	80	3.3810997E-4	16.1875	1
GTATGTA	70	0.0025916514	15.857142	1
TCTAAGC	70	0.0025916514	15.857142	3
CCGCCTA	70	0.0025916514	15.857142	15
GTATCAA	715	0.0	15.783216	2
GTATTAA	85	5.3618423E-4	15.235294	1
CCTTAAG	75	0.0041030827	14.8	1
GTATTAG	150	8.090137E-8	14.8	1
CGAAAGC	100	1.09276174E-4	14.8	19
>>END_MODULE