FastQCFastQC Report
Fri 10 Feb 2017
ERR1632033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446771
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT6050.1354161304113293No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT5260.11773369354770118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTA250.00549279229.65
GGTATCA2700.026.7222211
ACCGTCT400.001929349523.1250028
ATCTTAC502.6985208E-422.21
GCCGTCT1350.021.92592436
TCCGCTC450.003822203320.55555528
GTATTAG656.890068E-519.9230771
GTATCAA3750.019.242
AGACTGC701.2170816E-418.56
GACCGTG500.00702831618.57
GGATAAC500.00702831618.51
ACATCGC500.00702831618.58
TCTTATA8100.018.27160537
TCTTACA852.717086E-517.4117642
CAAGACT852.717086E-517.4117644
CCGTCTT1603.274181E-1117.3437537
TATGCCG1708.367351E-1116.3235333
ATGGACT700.002589169215.8571424
GCTTAAG700.002589169215.8571421
CTCTAGA700.002589169215.8571421
Produced by FastQC (version 0.11.5)