##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632033.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446771
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8748978783314	34.0	31.0	34.0	31.0	34.0
2	33.03977876809372	34.0	33.0	34.0	31.0	34.0
3	33.045840038856596	34.0	33.0	34.0	31.0	34.0
4	36.462530020972714	37.0	37.0	37.0	35.0	37.0
5	36.38472729877275	37.0	37.0	37.0	35.0	37.0
6	36.41552607487952	37.0	37.0	37.0	35.0	37.0
7	36.40630658659582	37.0	37.0	37.0	35.0	37.0
8	36.387992953884655	37.0	37.0	37.0	35.0	37.0
9	38.18808517115032	39.0	39.0	39.0	37.0	39.0
10	38.13424327004215	39.0	38.0	39.0	37.0	39.0
11	38.23341935801563	39.0	39.0	39.0	37.0	39.0
12	38.20523713490804	39.0	39.0	39.0	37.0	39.0
13	38.23086995351086	39.0	39.0	39.0	37.0	39.0
14	39.67037027918106	41.0	39.0	41.0	38.0	41.0
15	39.690326811722336	41.0	39.0	41.0	38.0	41.0
16	39.57573566771344	41.0	39.0	41.0	37.0	41.0
17	39.622130800790565	41.0	39.0	41.0	37.0	41.0
18	39.64135093817638	41.0	39.0	41.0	37.0	41.0
19	39.69599862121758	41.0	40.0	41.0	38.0	41.0
20	39.675200494212916	41.0	40.0	41.0	38.0	41.0
21	39.658666296603855	41.0	39.0	41.0	37.0	41.0
22	39.63659458648838	41.0	39.0	41.0	37.0	41.0
23	39.60472367275405	41.0	39.0	41.0	37.0	41.0
24	39.5553314785427	41.0	39.0	41.0	37.0	41.0
25	39.49717416752654	41.0	39.0	41.0	37.0	41.0
26	39.42222301805623	41.0	39.0	41.0	37.0	41.0
27	39.355746008581576	40.0	39.0	41.0	36.0	41.0
28	39.30875996875357	40.0	39.0	41.0	36.0	41.0
29	39.1640236273169	40.0	39.0	41.0	36.0	41.0
30	39.13349568347095	40.0	39.0	41.0	36.0	41.0
31	39.07993580603934	40.0	39.0	41.0	35.0	41.0
32	39.02116296715767	40.0	39.0	41.0	35.0	41.0
33	38.89499542271096	40.0	38.0	41.0	35.0	41.0
34	38.889585492343954	40.0	38.0	41.0	35.0	41.0
35	38.88255728326145	40.0	38.0	41.0	35.0	41.0
36	38.85936866985547	40.0	38.0	41.0	35.0	41.0
37	38.831070056024224	40.0	38.0	41.0	35.0	41.0
38	38.776191829818856	40.0	38.0	41.0	35.0	41.0
39	38.58787387722121	40.0	38.0	41.0	35.0	41.0
40	38.593478538222044	40.0	38.0	41.0	35.0	41.0
41	38.60475724700126	40.0	38.0	41.0	35.0	41.0
42	38.477222111551555	40.0	38.0	41.0	35.0	41.0
43	37.66484843465668	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	4.0
18	2.0
19	7.0
20	19.0
21	34.0
22	58.0
23	117.0
24	183.0
25	254.0
26	504.0
27	707.0
28	962.0
29	1398.0
30	1957.0
31	2839.0
32	3914.0
33	5752.0
34	8869.0
35	13956.0
36	23793.0
37	45044.0
38	108889.0
39	227506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95959227434189	17.584400061776613	12.23714162288958	27.21886604099192
2	19.738076106103573	19.22327098222579	33.42987794641998	27.608774965250653
3	20.759852362843603	20.549006090368444	28.878776823025664	29.812364723762286
4	15.709614097602575	14.993587318783	32.47860760881973	36.818190974794696
5	16.82696504473209	33.60043512224383	32.90880562972977	16.663794203294305
6	36.761786239482866	32.62879640800321	14.707087075929278	15.902330276584648
7	30.994178225533886	28.472528431791677	19.838351191102376	20.694942151572057
8	28.574817971623045	31.63029829599504	19.074648981245428	20.720234751136488
9	27.221775809083397	13.57944002632221	18.79329678963048	40.405487374963904
10	18.294159647783765	25.243357335189614	31.80577969474294	24.656703322283676
11	37.676572561782216	19.92989697182673	20.151263175094176	22.242267291296884
12	23.1393711767326	23.90665463962522	27.456795539549343	25.497178644092834
13	32.54709907312695	18.269090876534065	22.32709822257935	26.856711827759632
14	24.237472888795377	19.187906108498538	23.43594369374915	33.138677308956936
15	27.12978237172959	25.426672724953054	20.711505446862038	26.73203945645532
16	26.921845867345912	24.028417242838053	22.49273117547916	26.557005714336874
17	25.187847913136707	24.918582450517153	23.81085612092101	26.082713515425127
18	25.67198855789655	23.618587598568393	24.424817188223944	26.28460665531111
19	26.33496802612524	23.796531108778325	24.467792224652	25.400708640444435
20	26.76113713737015	23.50219687490907	23.897477678721312	25.839188308999468
21	27.098894064296918	23.38871591934123	23.386029979564473	26.126360036797376
22	27.224237920545423	23.72199627997341	23.33320649728832	25.720559302192846
23	26.679215974179165	23.319105313460366	23.344174084710065	26.657504627650408
24	26.581626828957116	23.63179346913743	23.298065451875793	26.48851425002966
25	26.456059144393883	23.243898999711263	23.691331800855473	26.60871005503938
26	26.227306606740363	23.42206633823592	23.715057602216795	26.63556945280692
27	25.98892945155348	23.760047093477418	23.685959921301965	26.56506353366714
28	26.095695557679438	23.51943165514324	23.87017062432432	26.514702162853006
29	25.91349930948965	23.853159672404878	24.2710471360048	25.962293882100674
30	25.94662590006961	23.888524546132135	23.927694501209793	26.237155052588463
31	26.278339462498685	23.593071170689235	23.563525833144944	26.56506353366714
32	25.60461623516298	23.84286356992732	24.184873234833955	26.367646960075742
33	25.471214559584215	23.254418930503544	24.64215448182626	26.632212028085977
34	26.054063491139758	22.778783761703423	24.541431740198	26.625721006958823
35	25.306924576572786	22.795794713622865	25.473005186102053	26.4242755237023
36	25.36064337210786	22.380593189799697	25.249400699687314	27.009362738405134
37	25.054670065872674	21.608385503983023	26.0186986174125	27.3182458127318
38	24.301040130178546	21.72141880292141	27.000857262445415	26.97668380445463
39	24.02125473676671	21.53049325045717	27.744638752291443	26.703613260484676
40	23.55703481201779	21.143270265975186	28.80245136770292	26.497243554304106
41	23.099753565025484	21.25137934198952	28.767981807234577	26.880885285750416
42	22.490045235702407	21.568096407331723	29.160129014640614	26.78172934232526
43	21.92353577112212	21.20818047724673	29.54578520091949	27.32249855071166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	14.0
2	18.0
3	39.0
4	60.0
5	60.0
6	78.0
7	96.0
8	102.5
9	109.0
10	156.5
11	204.0
12	204.0
13	363.5
14	523.0
15	779.0
16	1035.0
17	1025.5
18	1016.0
19	1016.0
20	1301.0
21	1586.0
22	1709.0
23	1832.0
24	2339.5
25	2847.0
26	2847.0
27	3419.0
28	3991.0
29	4788.5
30	5586.0
31	6917.5
32	8249.0
33	8249.0
34	9832.5
35	11416.0
36	13453.5
37	15491.0
38	16934.0
39	18377.0
40	18377.0
41	20399.5
42	22422.0
43	24094.0
44	25766.0
45	27088.5
46	28411.0
47	28411.0
48	29651.0
49	30891.0
50	31830.5
51	32770.0
52	33966.0
53	35162.0
54	35162.0
55	34844.5
56	34527.0
57	34451.0
58	34375.0
59	32859.5
60	31344.0
61	31344.0
62	29216.0
63	27088.0
64	24271.5
65	21455.0
66	18840.5
67	16226.0
68	16226.0
69	14116.0
70	12006.0
71	10298.0
72	8590.0
73	7074.5
74	5559.0
75	5559.0
76	4456.0
77	3353.0
78	2705.5
79	2058.0
80	1605.5
81	1153.0
82	1153.0
83	894.0
84	635.0
85	471.0
86	307.0
87	229.0
88	151.0
89	151.0
90	102.5
91	54.0
92	35.0
93	16.0
94	9.5
95	3.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	446771.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.75926617971925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56412160076302	74.95020245890007
2	6.448740273141287	10.673860255775502
3	1.3767551000052867	3.4181772535687056
4	0.5572654086093758	1.8447550513541338
5	0.2945433601535748	1.2188096172209304
6	0.18781240180810377	0.9325929931853547
7	0.11779814720209916	0.6824221753843392
8	0.09725678588272592	0.6439120184521979
9	0.06361814820365745	0.47384921349425846
>10	0.2809643083318029	3.890469290143782
>50	0.007868524919789956	0.4345031383714724
>100	0.0027132841493919297	0.5824811569350709
>500	5.426568298783729E-4	0.2539653772141706
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	605	0.1354161304113293	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	526	0.11773369354770118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2382831472946992E-4	0.0	0.0	0.0
3	0.0	4.4765662945893983E-4	0.0	0.0	0.0
4	0.0	4.4765662945893983E-4	0.0	2.2382831472946992E-4	0.0
5	0.0	4.4765662945893983E-4	0.0	2.2382831472946992E-4	0.0
6	0.0	4.4765662945893983E-4	0.0	2.2382831472946992E-4	0.0
7	0.0	4.4765662945893983E-4	0.0	2.2382831472946992E-4	0.0
8	0.0	4.4765662945893983E-4	0.0	2.2382831472946992E-4	0.0
9	0.0	4.4765662945893983E-4	0.0	4.4765662945893983E-4	0.0
10	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
11	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
12	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
13	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
14	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
15	0.0	6.714849441884097E-4	0.0	8.953132589178797E-4	0.0
16	0.0	8.953132589178797E-4	0.0	0.0013429698883768194	0.0
17	0.0	8.953132589178797E-4	0.0	0.002014454832565229	0.0
18	0.0	8.953132589178797E-4	0.0	0.002014454832565229	0.0
19	0.0	8.953132589178797E-4	0.0	0.002014454832565229	0.0
20	0.0	8.953132589178797E-4	0.0	0.002238283147294699	0.0
21	0.0	0.0011191415736473496	0.0	0.002462111462024169	0.0
22	0.0	0.0011191415736473496	0.0	0.0031335964062125785	0.0
23	0.0	0.0015667982031062893	0.0	0.004252737979859928	0.0
24	0.0	0.0017906265178357593	0.0	0.006938677756613567	0.0
25	0.0	0.0017906265178357593	0.0	0.008281647644990387	0.0
26	0.0	0.0017906265178357593	0.0	0.012758213939579785	0.0
27	0.0	0.0017906265178357593	0.0	0.04185589485441087	0.0
28	0.0	0.0017906265178357593	0.0	0.12735831108106838	0.0
29	0.0	0.002685939776753639	0.0	0.24755411609079372	0.0
30	0.0	0.002685939776753639	0.0	0.4181112919146498	0.0
31	0.0	0.002685939776753639	0.0	0.9476890845645756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGTA	25	0.005492792	29.6	5
GGTATCA	270	0.0	26.722221	1
ACCGTCT	40	0.0019293495	23.125002	8
ATCTTAC	50	2.6985208E-4	22.2	1
GCCGTCT	135	0.0	21.925924	36
TCCGCTC	45	0.0038222033	20.555555	28
GTATTAG	65	6.890068E-5	19.923077	1
GTATCAA	375	0.0	19.24	2
AGACTGC	70	1.2170816E-4	18.5	6
GACCGTG	50	0.007028316	18.5	7
GGATAAC	50	0.007028316	18.5	1
ACATCGC	50	0.007028316	18.5	8
TCTTATA	810	0.0	18.271605	37
TCTTACA	85	2.717086E-5	17.411764	2
CAAGACT	85	2.717086E-5	17.411764	4
CCGTCTT	160	3.274181E-11	17.34375	37
TATGCCG	170	8.367351E-11	16.32353	33
ATGGACT	70	0.0025891692	15.857142	4
GCTTAAG	70	0.0025891692	15.857142	1
CTCTAGA	70	0.0025891692	15.857142	1
>>END_MODULE