FastQCFastQC Report
Fri 10 Feb 2017
ERR1632032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322461
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3610.11195152281981387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGA250.00549055329.61
GGTATCA1500.027.1333331
TCTATAC358.855507E-426.428573
CTATACT451.3203299E-424.6666664
TAGACAG400.001928191723.1255
CCGGACT400.001928191723.1254
CTTATAC3450.021.44927437
CGGCCTT957.0976967E-921.42105324
TATACTT450.003819930420.5555555
ATACACA656.882465E-519.92307737
GTATCAA2800.019.1607131
TAGAGAG500.00702417818.55
TGGACAT609.217182E-418.55
TCCTAAC609.217182E-418.52
CCCCGTC609.217182E-418.59
CCATTTG701.21574885E-418.59
TCTTATA6350.017.48031437
CTTTGAG650.001576603217.0769232
TTCCTCG1301.38225005E-817.07692319
TCGCCAT803.3731342E-416.187513
Produced by FastQC (version 0.11.5)